[1]张人子,李晟,王导新.隐丹参酮减轻LPS诱导的ARDS小鼠早期肺纤维化及机制研究[J].陆军军医大学学报(原第三军医大学学报),2022,44(18):1819-1825.
　ZHANG Renzi,LI Sheng,WANG Daoxin.Effect and mechanism of cryptotanshinone on early pulmonary fibrosis in LPS-induced acute respiratory distress syndrome in mice [J].J Amry Med Univ (J Third Mil Med Univ),2022,44(18):1819-1825.
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隐丹参酮减轻LPS诱导的ARDS小鼠早期肺纤维化及机制研究(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
44卷
期数:
2022年第18期
页码:
1819-1825
栏目:
基础医学
出版日期:
2022-09-30

文章信息/Info

Title:
Effect and mechanism of cryptotanshinone on early pulmonary fibrosis in LPS-induced acute respiratory distress syndrome in mice 
作者:
张人子; 李晟; 王导新
重庆医科大学附属第二医院呼吸与危重症医学科
Author(s):
ZHANG Renzi;  LI Sheng;  WANG Daoxin

Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China

关键词:
急性呼吸窘迫综合征; 急性肺损伤; 隐丹参酮; 炎症; 内皮间质转化
Keywords:
acute respiratory distress syndrome;  acute lung injury;  cryptotanshinone;  inflammation;  endothelial-to-mesenchymal transition
分类号:
R285.5; R363.21; R563.8
文献标志码:
A
摘要:

目的探讨隐丹参酮（cryptotanshinone，CTS）对脂多糖（lipopolysaccharide，LPS）诱导的急性呼吸窘迫综合征（acute respiratory distress syndrome，ARDS）小鼠的早期肺纤维化的影响及其可能的作用机制。方法将40只8周龄雄性C57BL/6J小鼠随机分为正常组、模型组、处理组、CTS组（n=10）。模型组采用气管滴注LPS（5 mg/kg）制备ARDS模型。处理组在ARDS建模前腹腔注射CTS(60 mg/kg) 预处理3 d，建模后再连续予以CTS 7 d。正常组和CTS组分别注射等体积的无菌磷酸盐缓冲液（phosphate-buffered saline，PBS）或CTS。末次给药24 h后处死小鼠，取支气管肺泡灌洗液（bronchoalveolar lavage fluid，BALF）及肺组织。通过HE染色、肺组织湿/干质量（wet-to-dry，W/D）比值、BALF中蛋白浓度和总细胞计数评估肺组织损伤情况；采用酶联免疫吸附试验（ELISA）检测BALF中炎症因子水平；通过Masson染色及羟脯氨酸（hydroxyproline ，HYP）含量检测评估肺组织纤维化程度；利用免疫组化染色或Western blot检测VE-cadherin、CD31、波形蛋白（Vimentin）、α-平滑肌肌动蛋白（α-smooth muscle actin，α-SMA)的蛋白表达。结果与正常组比较，模型组的肺组织W/D、BALF中总细胞数胞计数和蛋白浓度升高，BALF中的TGF-β1、TNF-α、IL-1β、IL-6升高，IL-10降低（P<0.05），小鼠肺组织出现肺损伤和早期肺纤维化；HYP含量升高(P<0.05)；Vimentin、α-SMA的表达升高，而VE-cadherin、CD31的表达隆低（P<0.05）。与模型组比较，处理组的肺组织W/D、 BALF中总细胞计数和蛋白浓度降低，BALF 中的TGF-β1、TNF-α、IL-1β、IL-6降低，IL-10升高（P<0.05）；肺损伤和早期肺纤维化程度明显减轻，肺损伤评分和肺纤维化程度评分降低（P<0.05）；HYP含量显著降低（P<0.05）；Vimentin、α-SMA的表达降低，但VE-cadherin、CD31的表达增强（P<0.05）。结论CTS可能通过抑制LPS刺激后肺组织中的内皮间质转化（endothelial-to-mesenchymal transition，EndMT）过程从而减轻ARDS小鼠早期肺纤维化。

Abstract:

ObjectiveTo investigate the effects and underlying mechanism of cryptotanshinone (CTS) on early pulmonary fibrosis in mice with lipopolysaccharide (LPS)-induced acute respiratory distress syndrome (ARDS). MethodsForty male C57BL/6J mice (8 weeks old) were randomly divided into control group, model group, treatment group, and CTS group, with 10 mice in each group. A ARDS model was established by intratracheal instillation of LPS (5 mg/kg). The mice in the treatment group was intraperitoneally pretreated with CTS (60 mg/kg) for 3 d before modeling, followed by CTS administion for 7 consecutive days. Similarly, the mice of the control and CTS groups were injected with an equivalent volume of sterile phosphate-buffered saline (PBS) or CTS, respectively. The mice were sacrificed in 24 h after the last challenge, and bronchoalveolar lavage fluid (BALF) and lung tissues were collected. The injury of lung was observed with HE staining, lung wet-to-dry (W/D) ratio, total protein concentration and total cell number in BALF. The levels of inflammatory cytokines in BALF were determined by enzyme-linked immunosorbent assay (ELISA). The fibrosis of lung was characterized by Masson staining and content of hydroxyproline (HYP). The protein levels of VE-cadherin, CD31, Vimentin and α-smooth muscle actin(α-SMA) were detected by immunohistochemistry or Western blotting. ResultsObviously increased lung W/D ratio and total protein concentration and total cell number in BALF, elevated contents of TNF-α, TGF-β1, IL-1β and IL-6 while reduced content of IL-10 in BALF were seen in the model group when compared with the control group (P<0.05).  Compared with the model group, the treatment group had significantly decreased lung W/D ratio and total protein concentration and total cell number in BALF, down-regulated levels of IL-1β, IL-6, TNF-α and TGF-β1 and up-regulated level of IL-10 in BALF (P<0.05). In addition, CTS also ameliorated lung injury and early fibrosis, decreased lung injury and fibrosis score, and reduced HYP content in LPS-induced ARDS (P<0.05), up-regulated the VE-cadherin and CD31 levels, and suppressed the Vimentin and α-SMA levels (P<0.05). ConclusionCTS alleviates LPS-induced early pulmonary fibrosis in ARDS mice, and its underlying mechanism may be related to the inhibition of endothelial-to-mesenchymal transition (EndMT).

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更新日期/Last Update: 2022-09-23