[1]马秦龙,陈纯海,林敏,等.0.22太赫兹电磁辐射暴露致神经母细胞瘤Neuro-2a细胞损伤的非热效应研究[J].第三军医大学学报,2020,42(23):2267-2273.
 MA Qinlong,CHEN Chunhai,LIN Min,et al.Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells[J].J Third Mil Med Univ,2020,42(23):2267-2273.
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0.22太赫兹电磁辐射暴露致神经母细胞瘤Neuro-2a细胞损伤的非热效应研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第23期
页码:
2267-2273
栏目:
专题报道
出版日期:
2020-12-15

文章信息/Info

Title:
Non-thermal effects of 0.22 terahertz electromagnetic radiation exposure-induced injury in Neuro-2a cells
作者:
马秦龙陈纯海林敏陶嘉雯邓平高鹏卢永辉皮会丰何旻蒂张蕾张彦文余争平
陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室,电磁辐射医学防护教育部重点实验室
Author(s):
MA Qinlong CHEN Chunhai LIN Min TAO Jiawen DENG Ping GAO Peng LU Yonghui PI Huifeng HE Mindi ZHANG Lei ZHANG Yanwen YU Zhengping

Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China

关键词:
太赫兹辐射细胞增殖细胞凋亡突触生长Neuro-2a细胞
Keywords:
terahertz radiation cell proliferation cell apoptosis neurite outgrowth Neuro-2a cell
分类号:
R-334; R146; R730.264
文献标志码:
A
摘要:

目的探讨0.22太赫兹(terahertz,THz)电磁辐射暴露致小鼠神经母细胞瘤细胞株——Neuro-2a细胞损伤的非热效应特点。方法以Neuro-2a细胞作为体外实验暴露模型,采用频率为0.22 THz,平均功率密度为0(Sham组)、25(25 mW/cm2辐照组)、50 mW/cm2(50 mW/cm2辐照组)辐照细胞,辐照时间为5 min,监测辐照引起的细胞培养基温度变化;于暴露后24 h检测细胞增殖(EdU阳性细胞比例和细胞活力)、凋亡(γH2AX、TUNEL阳性细胞比例、Bax与Bcl2基因表达水平);于暴露后48 h分析细胞突触生长(突触长度、分支数目及Tubb3与Syp基因表达水平)的变化情况。结果与Sham组比较,两辐照组细胞培养基温度升高在0.1~0.4 ℃;EdU阳性细胞比例、细胞活力、γH2AX和TUNEL阳性细胞比例及Bax、Bcl2基因表达水平均无明显改变;仅50 mW/cm2辐照组细胞突触长度和分支数目较Sham组明显减少(P<0.01),Tubb3和Syp基因表达也明显降低(P<0.05)。结论THz辐射暴露以非热效应为主,可抑制Neuro-2a细胞突触生长,而不影响细胞增殖和凋亡,提示神经细胞突触生长是THz辐射产生神经生物效应的敏感靶标。

Abstract:

ObjectiveTo explore the features of non-thermal effects  of cell injury induced by terahertz (THz) radiation at a frequency of 0.22 THz, in a mouse neuroblastoma cell line, Neuro-2a. MethodsThe cultured Neuro-2a cells were exposed to 0.22 THz irradiation at average power densities of 0 (sham group), and 25 and 50 mW/cm2 (radiation groups), for 5 min, respectively. The temperature changes of culture medium were monitored. The cell proliferation and apoptosis and the mRNA expression of Bax and Bcl2 at 24 h after THz exposure were detected by measuring the ratio of EdU+ cells, CCK-8 assay for cell viability, immunofluorescence assay for γH2AX+ cells, TUNEL assay, and real-time PCR. The neurite length, number of branch points, and mRNA expression of Tubb3 and Syp were analyzed in the Neuro-2a cells at 48 h after THz exposure. ResultsCompared with the sham group, the temperature of culture medium in the radiation groups was increased by a range from 0.1 to 0.4 ℃. There were no significant differences in the cell viability, ratios of positive cells for EdU, γH2AX and TUNEL, or the mRNA expression levels of Bax and Bcl2. The neurite length and number of branch points were obviously reduced (P<0.01), and the mRNA levels of Tubb3 and Syp were also notably down-regulated (P<0.05) in the 50 mW/cm2 group. ConclusionTHz radiation mainly exhibits non-thermal effects, and can inhibit neurite outgrowth but not affect the proliferation and apoptosis in Neuro-2a cells. Our findings suggest that neurite outgrowth is a sensitive target of neurobiological effects after THz radiation exposure. 

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更新日期/Last Update: 2020-12-04