[1]陈纯海,马秦龙,陶嘉雯,等.太赫兹辐射暴露致小鼠皮肤损伤效应研究[J].第三军医大学学报,2020,42(23):2282-2289.
 CHEN Chunhai,MA Qinlong,TAO Jiawen,et al.Effects of terahertz exposure on skin injury in mouse model[J].J Third Mil Med Univ,2020,42(23):2282-2289.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第23期
页码:
2282-2289
栏目:
专题报道
出版日期:
2020-12-15

文章信息/Info

Title:
Effects of terahertz exposure on skin injury in mouse model
作者:
陈纯海马秦龙陶嘉雯卢永辉林敏高鹏邓平何旻蒂皮会丰张蕾张彦文余争平
陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室,电磁辐射医学防护教育部重点实验室
Author(s):
CHEN Chunhai MA Qinlong TAO Jiawen LU Yonghui LIN Min GAO Peng DENG Ping HE Mindi PI Huifeng ZHANG Lei ZHANG Yanwen YU Zhengping

Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China

关键词:
太赫兹皮肤热效应创伤与损伤RNA-seq炎症反应
Keywords:
terahertz skin thermal effect wounds and injuries RNA-seq analysis inflammatory response
分类号:
R-334; R146; R758.13
文献标志码:
A
摘要:

目的探讨太赫兹(terahertz,THz)辐射暴露所致皮肤组织损伤的生物效应特点及其潜在的分子机制。方法采用C57BL/6J小鼠为实验动物模型,通过频率为0.22 THz,平均功率密度为0、25、50 mW/cm2的THz辐照动物,辐照时间为5 min,观察辐照引起的暴露部位皮肤升温曲线特点和皮肤组织病理损伤特征,同时通过对辐照部位皮肤组织进行转录组测序及生物信息学分析,揭示损伤的整体效应及潜在分子机制,并进一步通过ELISA验证RNA-seq揭示的相关效应。结果25 mW/cm2 THz辐照不引起明显的升温效应,但50 mW/cm2 THz辐照能够导致辐照动物皮肤平均温度迅速升高超过2 ℃,并且导致辐照部位皮肤组织出现上皮脱落及出血等病理损伤特征。通过对损伤皮肤组织进行转录组测序,共筛选出上调基因49个,下调基因27个。对这些基因进行GO及KEGG分析结果显示,其主要功能为调控机体组织免疫及炎症反应等。ELISA检测结果显示,THz暴露后皮肤炎症因子IL-1β、IL-6及TNF-α表达明显升高(P<0.05,P<0.01)。结论THz辐射导致暴露部位皮肤出现明显的热效应损伤及引起炎症反应等损伤效应,并导致一系列基因表达异常。

Abstract:

ObjectiveTo explore the characteristics of biological effects of terahertz (THz) exposure on the skin injury, and investigate the underlying mechanisms. MethodsThe hair-removed skin of C57BL/6J mice were exposed to 0.22 THz irradiation, at an average power density of 0, 25 and 50 mW/cm2, respectively, for 5 min. The temperature curve and pathological changes in the exposed area were observed and analyzed. The gene expression in the skin area was detected using RNA-seq analysis and bioinformatics analysis for global changes of the impairment and underlying molecular mechanisms. Finally, ELISA was used to verify inflammatory responses in the skin revealed by RNA-seq. ResultsThough 25 mW/cm2 THz irradiation did not induce obvious temperature change, 50 mW/cm2 THz irradiation exposure significantly raised the temperature higher than 2 ℃, and caused epithelial detachment and bleeding in the exposed area. RNA-seq analysis found 49 genes up-regulated and 27 genes down-regulated in the injured skin area. The following Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these genes were majorly involved in immune responses and inflammatory responses. The results of ELISA indicated that the expression levels of IL-1β, IL-6, and TNF-α were increased remarkably after exposure. ConclusionTHz irradiation exposure causes significant thermal effects and other injuries, including inflammatory responses, and induces abnormal expression of a series of genes in the exposed skin area. 

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更新日期/Last Update: 2020-12-04