[1]邓永,唐腾骞,张庆仪,等.DIDS通过抑制氯离子通道2型的开放缓解肝缺血再灌注损伤[J].第三军医大学学报,2020,42(24):2375-2381.
 DENG Yong,TANG Tengqian,ZHANG Qingyi,et al.DIDS alleviates hepatic ischemia-reperfusion injury by inhibiting the opening of type 2 chloride channel in rats[J].J Third Mil Med Univ,2020,42(24):2375-2381.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第24期
页码:
2375-2381
栏目:
基础医学
出版日期:
2020-12-30

文章信息/Info

Title:
DIDS alleviates hepatic ischemia-reperfusion injury by inhibiting the opening of type 2 chloride channel in rats
作者:
邓永唐腾骞张庆仪张雷达
陆军军医大学(第三军医大学)第一附属医院肝胆外科
Author(s):
DENG Yong TANG Tengqian ZHANG Qingyi ZHANG Leida

Department of Hepatobiliary Surgery, First Affiliated Hospital, Army Military Medical University (Third Military Medical University), Chongqing, 400038, China

关键词:
肝脏缺血再灌注损伤DIDSClC2
Keywords:
liver ischemia-reperfusion injury 44&prime-diisothiocyanostilbene-22&prime-disulfonic acid type 2 chloride channel
分类号:
R364.12;R657.3;R966
文献标志码:
A
摘要:

目的探讨氯离子通道2型(type 2 chloride channel, ClC2)及其阻断剂4,4-二异硫氰基芪-2,2’-二磺酸(4,4’-diisothiocyanostilbene-2,2’-disulfonic acid, DIDS)在肝缺血再灌注损伤中的作用。方法成年SD大鼠以经典法建立肝缺血再灌注模型。采用q-PCR技术对缺血再灌注后肝组织中ClC2的表达进行检测,采用肝功能血生化分析、活性氧浓度检测、ELISA及流式细胞技术观察缺血再灌注及应用DIDS后对肝功能、氧自由基水平、炎性细胞因子及细胞周期等方面的影响。结果缺血再灌注后肝组织中ClC2 mRNA表达显著升高(P<0.05),并伴随有活性氧浓度升高(P<0.05)、谷丙及谷草转氨酶升高(P<0.05)和炎性细胞因子浓度升高。术后即时使用DIDS组相比缺血再灌注组在ClC2表达、转氨酶浓度、活性氧水平及炎性因子表达方面均有所逆转(P<0.05),术后8 h应用DIDS组相比缺血再灌注组在ClC2表达、转氨酶浓度及iNOS(一氧化氮合酶)表达方面有显著差异(P<0.05),而预用DIDS 1 h组相比缺血再灌注组未观察到统计学差异。结论DIDS可以对肝缺血再灌注损伤引起的肝实质损害、炎性反应及氧化应激起保护作用。

Abstract:
ObjectiveTo investigate the roles of type 2 chloride channel (ClC2) and its blocker, 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS), in rat model of hepatic ischemia-reperfusion (I/R) injury. MethodsA total of 75 health adult SD rats were randomly divided into sham operation group, hepatic I/R injury group, I/R+immediate treatment of DIDS group, I/R+8 h later treatment of DIDS group, and 1 h pretreatment of DIDS+I/R group, with 15 animals in each group. The rat model of hepatic I/R injury was established by classical method, and DIDS was given by intraperitoneal injection at a dose of 50 mg/kg at corresponding time points. The expression of ClC2 in the liver tissues was detected with q-PCR. Then blood biochemical analysis, detection of reactive oxygen concentration, ELISA and flow cytometry were employed respectively to analyze and compare the liver function, level of reactive oxygen species (ROS), contents of inflammatory cytokines and cell cycle of hepatic cells among the different groups. ResultsThe rats with hepatic I/R injury had significantly enhanced expression of ClC2 mRNA (P<0.05), increased production of ROS (P<0.05), elevated contents of inflammatory cytokines in liver tissues, and raised serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (P<0.05). However, all above phenomena were reversed in the rats from the I/R+immediate treatment of DIDS group when compared with the model group (P<0.05). Significant differences were also observed in the ClC2 expression, transaminase concentrations, and inducible nitric oxide synthase (iNOS) level between the model group and the group using DIDS 8 h after operation (P<0.05). But no statistical difference was found in comparison with the group undergoing pretreatment of DIDS 1 h before operation. ConclusionDIDS has a protective effect on liver parenchymal damage, inflammatory reaction and oxidative stress induced by hepatic I/R injury.
 

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更新日期/Last Update: 2020-12-22