[1]刘博,黄露,曹亚,等.叶酸受体阳性CTC检测在恶性肺部结节辅助诊断中的应用[J].陆军军医大学学报(原第三军医大学学报),2020,42(23):2330-2335.
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叶酸受体阳性CTC检测在恶性肺部结节辅助诊断中的应用(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第23期
页码:
2330-2335
栏目:
临床医学
出版日期:
2020-12-15

文章信息/Info

Title:
Application of folate receptor-positive circulating tumor cells in auxiliary diagnosis of malignant pulmonary nodules
作者:
刘博; 黄露; 曹亚; 谭秋林; 王亚丽; 郭乔楠
陆军军医大学(第三军医大学)第二附属医院病理科
Author(s):
LIU Bo;  HUANG Lu;  CAO Ya;  TAN Qiulin;  WANG Yali;  GUO Qiaonan

Department of Pathology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, China

关键词:
叶酸受体; 循环肿瘤细胞; 肺部结节; 肺癌; 辅助诊断
Keywords:
folate receptor;  circulating tumor cells;  pulmonary nodules;  lung cancer;  auxiliary diagnosis
文献标志码:
A
摘要:

目的评价靶向叶酸受体（folate receptor, FR）阳性循环肿瘤细胞（circulating tumor cell, CTC）检测方法在临床肺部结节良恶性诊断中的实用价值。方法选取 2017年5月至2018年4月本院收治的肺部结节筛查患者288例，采用靶向FR CTC技术检测外周血中CTC含量；结合患者术后的病理检查结果，分析靶向FR CTC检测在肺部恶性肿瘤辅助诊断中的准确性、可行性。结果以8.7 Folate Units/3 mL为cut-off值，136例肺部恶性肿瘤的CTC水平显著高于152例肺部良性疾病患者（P<0.05）。136例肺部恶性肿瘤FR阳性CTC 107例，152例良性病变结节FR假阳性CTC 44例，总体上靶向FR CTC检测技术对肺部恶性肿瘤的检测灵敏度为78.7%，特异性为71.1%。特别是在Ⅰ期肺癌灵敏度达到76.9%，9例原位肺癌CTC检测均呈阳性，与肿瘤标志物、CT检查等比较，CTC检测对早期肺癌具有较高的辅助诊断价值。 结论靶向FR CTC检测可以应用于肺部结节良恶性的筛查，对肺部恶性肿瘤的早发现有较好的辅助诊断价值。

Abstract:

ObjectiveTo evaluate the practical value of detecting folate receptor (FR)-positive circulating tumor cells (CTC) in clinical diagnosis of benign and malignant lung nodules. MethodsA total of 288 patients with pulmonary nodules admitted in our hospital from May 2017 to April 2018 were enrolled in this study. FR-targeted CTC technology was used to quantitatively detect CTC content in peripheral blood samples. The results were analyzed with the postoperative pathological results to evaluate the accuracy and feasibility of the detection in the auxiliary diagnosis of lung malignant tumors. ResultsWith a cut-off value of 8.7 Folate Units/3 mL as the threshold, the CTC content was significantly higher in the 136 patients with pulmonary malignant tumor than the 152 patients with pulmonary benign disease (P<0.05). Among the 136 patients with pathologically diagnosed pulmonary malignant tumors, FR-positive CTC were observed in 106 patients. In the 152 patients with benign nodules, 44 cases showed false positive CTC results. In general, the sensitivity and specificity of FR-positive CTC detection was 78.7% and 71.1%, respectively in the detection of pulmonary malignant tumors. Furthermore, the sensitivity reached 76.9% in stage I lung cancer (70/91), and all 9 cases of lung cancer in situ were positive in the detection. Compared with tumor markers and CT examinations, FR-positive CTC detection was of higher auxiliary diagnostic value for early lung cancer. ConclusionFR-positive CTC detection can be used to screen benign and malignant lung nodules, and has a good value in the diagnosis of early-stage pulmonary malignant tumors. 

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更新日期/Last Update: 2020-12-04