[1]郭俊恺,赵兴旺,倪兵,等.3种环状RNA在系统性红斑狼疮中的表达及临床意义[J].第三军医大学学报,2020,42(24):2387-2393.
 GUO Junkai,ZHAO Xingwang,NI Bing YOU Yi.Expression and clinical significance of 3 kinds of circular RNA in systemic lupus erythematosus[J].J Third Mil Med Univ,2020,42(24):2387-2393.
点击复制

3种环状RNA在系统性红斑狼疮中的表达及临床意义(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第24期
页码:
2387-2393
栏目:
临床医学
出版日期:
2020-12-30

文章信息/Info

Title:
Expression and clinical significance of 3 kinds of circular RNA in systemic lupus erythematosus
作者:
郭俊恺赵兴旺倪兵游弋
陆军军医大学第一附属医院皮肤科;陆军军医大学病理生理学教研室;
 
Author(s):
GUO Junkai ZHAO Xingwang NI Bing YOU Yi
Department of Dermatology, First Affiliated Hospital; 2Department of Pathophysiology, Army Medical University (Third Military Medical University), Chongqing, 400038, China
 
关键词:
系统性红斑狼疮外周血单个核细胞环状RNA 
Keywords:
 
分类号:
R363.21;R394.3;R593.241
文献标志码:
A
摘要:
目的探讨三种环状RNA(circRNA)在系统性红斑狼疮(systemic lupus erythematosus, SLE)患者中的表达及临床意义,为揭示SLE 的临床诊断、治疗提供新的思路和方向。方法筛选35例SLE患者(实验组)及25例健康对照者(对照组),提取外周血单个核细胞(PBMCs),采用反转录定量PCR(RT-qPCR)检测circADCY9,circGARS,circMCTP2的表达水平,分析3种circRNA表达水平与临床活动度指标之间的相关性,采用受试者工作特征曲线(ROC)评价3种circRNA的诊断价值,通过核酸外切酶RNaseR处理检测3种circRNA分子的稳定性及耐受程度,选取circADCY9分子PCR验证其稳定性。结果①RT-qPCR分析结果显示 circADCY9 和circGARS在SLE患者PBMCs中表达上调,circMCTP2表达下调(P值均<0.000 1);②SLE患者circADCY9,circGARS表达水平与SLE疾病活动指数(SLEDAI)评分呈正相关,与补体C3呈负相关,circMCTP2表达水平与SLEDAI评分呈负相关,与补体C3呈正相关;③SLE 患者组和健康对照组circADCY9、circGARS、circMCTP2 分子ROC曲线下面积(AUC)分别为0.850 0、0.826 7、0.895 6;④RNaseR处理前后3种circRNA表达情况较为稳定,PCR显示:circRNA相较于mRNA对RNaseR的耐受性更好。结论SLE患者PBMCs中circADCY9,circGARS,circMCTP2表达明显异常,且稳定性好,与SLE疾病活动度具有相关性。
 
Abstract:

ObjectiveTo explore the expression and significance of 3 circular RNAs (circRNA), that is, circADCY9, circGARS and circMCTP2, in the patients with systemic lupus erythematosus (SLE), so as to provide new ideas and directions for its clinical diagnosis and treatment. MethodsPeripheral blood mononuclear cells (PBMCs) were extracted from the SLE patients (n=35) and healthy volunteers (n=25, control group). The expression levels of above 3 circRNAs were detected by real-time quantitative PCR (RT-qPCR), and their correlations with SLE Disease Activity Index (SLEDAI) were analyzed. The diagnostic value of the 3 circRNAs was evaluated by receiver operating characteristic (ROC) curve. Their stability and tolerance were detected using ribonuclease R (RNaseR) treatment, and the stability of circADCY9 was further verified by PCR analysis. Results① The levels of circADCY9 and circGARS were up-regulated, while that of circMCTP2 was down-regulated (All P values were <0.000 1) in the SLE patients than the healthy volunteers. ② In SLE patients, the expression levels of circADCY9 and circGARS were positively correlated with the score of SLEDAI, and negatively with the level of complement C3. By contrast, the circMCTP2 level was negatively correlated with SLEDAI score and positively with complement C3 level. ③ The area under the curve (AUC) for circADCY9, circGARS and circMCTP2 were 0.850 0, 0.826 7 and 0.895 6, respectively. ④ The expression levels of the 3 circRNAs remained steady before and after RNaseR treatment, and the result of PCR showed that the tolerance of circADCY9 to RNaseR was better than that of ADCY9 mRNA. ConclusionThe expression of circADCY9, circGARS and circMCTP2 is significantly abnormal in the PBMCs of SLE patients, and shows good stability, which is correlated with the activity of SLE. 

相似文献/References:

[1]熊仕秋,王莉,李明昌,等.无症状乙型肝炎病毒携带者外周血单个核细胞分泌细胞因子的研究[J].第三军医大学学报,2007,29(16):1580.
 XIONG Shi-qiu,WANG Li,LI Ming-chang,et al.Study on the cytokines induced from PBMCs of asymptomatic HBV carriers[J].J Third Mil Med Univ,2007,29(24):1580.
[2]王林纤,戴勇,彭武建,等.丝氨酸-苏氨酸激酶受体相关蛋白在健康人和系统性红斑狼疮患者外周血单个核细胞中的差异表达[J].第三军医大学学报,2011,33(02):148.
 Wang Linqian,Dai Yong,Peng Wujian,et al.Differential expression of serine-threonine kinase receptor-associated protein in healthy subjects and patients with systemic lupus erythematosus[J].J Third Mil Med Univ,2011,33(24):148.
[3]王新国,刘杞,孙航.人肝再生增强因子对体外单个核细胞PKCα-NF-кB及I-2的影响[J].第三军医大学学报,2009,31(21):2065.
 WANG Xin-guo,LIU Qi,SUN Hang.Effects of human augmenter of liver regeneration on PKCα-NF-κB expressions and IL-2 secretion in peripheral mononuclear cells[J].J Third Mil Med Univ,2009,31(24):2065.
[4]周云涛,刘静,王志国,等.类风湿关节炎外周血CD4+CD161+T细胞亚群频数情况[J].第三军医大学学报,2012,34(05):438.
 Zhou Yuntao,Liu Jing,Wang Zhiguo,et al.Frequency of peripheral blood CD161+CD4+ T cells in rheumatoid arthritis[J].J Third Mil Med Univ,2012,34(24):438.
[5]杨先涛,张祯祯,詹学,等.肺结核患儿外周血单个核细胞中microRNA表达的初步研究[J].第三军医大学学报,2013,35(19):2051.
 Yang Xiantao,Zhang Zhenzhen,Zhan Xue,et al.microRNA expression in peripheral blood mononuclear cells from pulmonary tuberculosis children[J].J Third Mil Med Univ,2013,35(24):2051.
[6]蔡安季,戴勇,李武县,等.强直性脊柱炎患者外周血单个核细胞中急性时相反应蛋白的差异表达研究[J].第三军医大学学报,2013,35(19):2084.
 Cai Anji,Dai Yong,Li Wuxian,et al.Expression of acute phase proteins in mononuclear cells from ankylosing spondylitis patients[J].J Third Mil Med Univ,2013,35(24):2084.
[7]牛军,钟华,宋志强,等.PBMC中CD69、CD107a表达上调在诊断迟发型药物变态反应中的意义[J].第三军医大学学报,2012,34(06):508.
 Niu Jun,Zhong Hua,Song Zhiqiang,et al.Significance of CD69 and CD107 upregulations on PBMC for delayed-type drug hypersensitivity diagnosis[J].J Third Mil Med Univ,2012,34(24):508.
[8]阎衡,宋志强,向明明,等.CTLA4Ig抑制银屑病外周血单个核细胞增殖的体外研究[J].第三军医大学学报,2004,26(04):0.[doi:10.16016/j.1000-5404.2004.04.021 ]
 YAN Heng,SONG Zhi qiang,XIANG Ming ming,et al.[J].J Third Mil Med Univ,2004,26(24):0.[doi:10.16016/j.1000-5404.2004.04.021 ]
[9]许礼发,李朝品,王健,等.结核病患者mIL-2R、sIL-2R表达的检测及其临床意义[J].第三军医大学学报,2004,26(04):0.[doi:10.16016/j.1000-5404.2004.04.040 ]
[10]程虹,刘彦仿,张惠中,等.sFv-TNF-α基因修饰的PBMCs对人肝癌细胞系HHCC的体外杀伤活性研究[J].第三军医大学学报,2003,25(14):0.[doi:10.16016/j.1000-5404.2003.14.021 ]
 CHENG Hong,LIU Yan fang,ZHANG Hui zhong,et al.[J].J Third Mil Med Univ,2003,25(24):0.[doi:10.16016/j.1000-5404.2003.14.021 ]

更新日期/Last Update: 2020-12-22