[1]吕丹,孙莹霄,欧阳丽萍,等.磺化聚醚醚酮-他汀缓释系统促进骨修复的作用研究[J].第三军医大学学报,2020,42(19):1907-1912.
 LYU Dan,SUN Yingxiao,OUYANG Liping,et al.Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect[J].J Third Mil Med Univ,2020,42(19):1907-1912.
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磺化聚醚醚酮-他汀缓释系统促进骨修复的作用研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第19期
页码:
1907-1912
栏目:
基础医学
出版日期:
2020-10-15

文章信息/Info

Title:
Sulfonated PEEK-statins delivery system enhances bone repair in mice with skull defect
作者:
吕丹孙莹霄欧阳丽萍李玲王鲁萍廖赟
上海交通大学医学院附属同仁医院药学部
 
Author(s):
LYU Dan SUN Yingxiao OUYANG Liping LI Ling WANG Luping LIAO Yun

Department of Pharmacy, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200336, China

关键词:
成骨成血管磺化PEEK辛伐他汀普伐他汀
Keywords:
 
分类号:
R318.08;R681;R945
文献标志码:
A
摘要:

目的探索磺化聚醚醚酮(poly-ether-ether-ketone, PEEK)-他汀缓释系统对骨损伤的修复效果,及对成骨和H型血管的影响。方法建立小鼠颅骨损伤模型;构建磺化PEEK(SP)-普伐他汀(Pra)和SP-辛伐他汀(Sim)局部缓释系统;将不同浓度梯度的SP、SP-Pra 0.16 mg/mL、SP-Sim 0.55 mg/mL、SP-Sim 1.1 mg/mL、SP-Sim 2.2 mg/mL 5组材料植入小鼠颅骨骨缺损处,8周后取材,micro-CT检测样品周围骨再生情况,CD31和EMCN荧光双染分析样品周围骨特异性H型血管表达。结果磺化PEEK可实现他汀药物的有效负载。micro-CT结果显示:SP-Sim 1.1 mg/mL组及SP-Sim 0.55 mg/mL组材料植入组新骨再生厚度显著高于其他3组小鼠,未负载药物的SP组新骨再生厚度最低。免疫荧光检测发现,SP-Sim 1.1 mg/mL组小鼠植入物周围颅骨中CD31和EMCN双阳性H型血管明显可见,而SP组及SP-Pra 0.16 mg/mL组未观察到典型的H型血管。结论SP-他汀缓释系统在实现骨再生治疗的同时可优化SP材料的成骨性能,机制可能与其对骨H型血管的保护作用相关。

Abstract:

ObjectiveTo explore the effect of sulfonated poly-ether-ether-ketone (SPEEK)-statin delivery system on the repair of bone defect, H-type blood vessels and bone formation. MethodsThe mouse model of skull defect was established, SPEEK-pravastatin (SPEEK-Pra) and sulfonated SPEEK-simvastatin (SPEEK-Sim) delivery system were constructed, respectively. Then the samples with different concentration gradients (SPEEK 0.16 mg/mL, SPEEK -Pra 0.16 mg/mL, SPEEK -Sim 0.55 mg/mL, SPEEK -Sim 1.1 mg/mL, SPEEK -Sim 2.2 mg/mL) were implanted into the skull defect of mice, respectively. After 8 weeks, the skull samples were collected, and micro-CT scanning was used to observe the bone regeneration around of the defects, and CD31 and EMCN immunofluorescent staining was utilized to label H-type blood vessels. ResultsSPEEK can be used as a payload for statins. Micro-CT results showed that the thickness of skull were significantly larger in SPEEK-sim 1.1 mg/mL and SPEEK-sim 0.55 mg /mL groups than the other 3 groups, with that of the SPEEK group (without drug loading) lowest. Immunofluorescence assay found that the double-positive area was clearly visible in the SPEEK-sim 1.1 mg /mL group, but not observed in the SPEEK group and the SPEEK-pra 0.16 mg/mL group. ConclusionSPEEK-statin sustained-release system optimizes the osteogenic properties of SPEEK, which may be related to the protective effect of H blood vessel. 

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更新日期/Last Update: 2020-10-02