[1]林子越,张攀扬,崇馨煜,等.半乳糖凝集素3调控高糖下肾小球系膜细胞增殖的研究[J].第三军医大学学报,2020,42(19):1913-1919.
 LIN Ziyue,ZHANG Panyang,CHONG Xinyu,et al.Galectin-3 regulates proliferation of glomerular mesangial cells under high glucose condition[J].J Third Mil Med Univ,2020,42(19):1913-1919.
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半乳糖凝集素3调控高糖下肾小球系膜细胞增殖的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第19期
页码:
1913-1919
栏目:
基础医学
出版日期:
2020-10-15

文章信息/Info

Title:
Galectin-3 regulates proliferation of glomerular mesangial cells under high glucose condition
作者:
林子越张攀扬崇馨煜孙艳彭睿张政
重庆医科大学基础医学院:细胞生物学及遗传学教研室1,生物信息学教研室
Author(s):
LIN Ziyue ZHANG Panyang CHONG Xinyu SUN Yan PENG Rui ZHANG Zheng

Department of Cell Biology and Genetics, 2Department of Bioinformatics, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China

关键词:
糖尿病肾病galectin-3肾小球系膜细胞细胞增殖Mek
Keywords:
diabetic nephropathy galectin-3 glomerular mesangial cells proliferation Mek
分类号:
R322.61;R329.28;R589.1
文献标志码:
A
摘要:

目的探究半乳糖凝集素3(galectin-3,Gal-3)对高糖培养的肾小球系膜细胞(mesangial cells,MCs)增殖的作用及机制。方法实时定量PCR(qRT-PCR)检测Gal-3在糖尿病肾病(diabetic nephropathy,DN)、正常小鼠肾脏组织和高低糖条件下MCs中的表达;构建、合成Gal-3过表达质粒和siRNA,qRT-PCR和Western blot检测转染效率;在高糖培养的MCs中转染Gal-3-siRNA;在低糖培养的MCs中转染Gal-3过表达质粒,通过5-乙基-2′-脱氧尿嘧啶核苷(5-Ethynyl-2′-deoxyuridine, EdU)检测MCs的增殖能力,通过Western blot检测高低表达Gal-3对Mek和磷酸化Mek表达的影响。 结果qRT-PCR结果显示:与正常小鼠相比,Gal-3在DN小鼠肾脏组织中表达显著升高(P<0.01),且与低糖下的MCs相比,高糖条件下的MCs中Gal-3表达显著升高(P<0.01)。过表达Gal-3后,MCs增殖能力显著提高(P<0.01)且磷酸化Mek表达上调(P<0.01),而在siRNA沉默Gal-3后,MCs增殖能力显著降低(P<0.01)且磷酸化Mek表达下调(P<0.001)。结论Gal-3在DN中显著上调,并且可能通过调控Mek磷酸化影响MCs的增殖,进而在DN中发挥重要的调节作用。

Abstract:

ObjectiveTo investigate the effect and underlying mechanism of galectin-3 (Gal-3) on the proliferation of mesangial cells (MCs) cultured under high glucose condition. MethodsReal-time quantitative PCR (qRT-PCR) was used to detect the expression of Gal-3 in normal and diabetic nephropathy (DN) mouse kidney tissues and MCs under low and high glucose conditions. Gal-3 overexpression plasmid and siRNA were constructed and synthesized. qRT-PCR and Western blotting were used to detect the transfection efficiency. And Gal-3 overexpression plasmid or siRNA was transfected into MCs under low and high glucose condition, respectively. Further, 5-Ethynyl-2′-deoxyuridine (EdU) was used to detect the cell proliferation ability, and Western blot assay was used to measure the expression of Mek and phosphorylated Mek after overexpression or downexpression of Gal-3 in MCs. ResultsqRT-PCR results showed that Gal-3 expression was significantly higher in the kidney tissues of DN mice than those of the normal mice(P<0.01), and so was the expression level in MCs under high glucose condition when compared with the cells under low glucose condition (P<0.01). In addition, overexpression of Gal-3 resulted in enhanced proliferation ability of MCs (P<0.01) and the up-regulation of phosphorylated Mek (P<0.01). While, Gal-3 silencing induced the opposite results in the proliferation ability  (P<0.01) and phosphorylated Mek expression (P<0.001). ConclusionGal-3 is significantly up-regulated in DN, and may affect the proliferation of MCs by regulating Mek phosphorylation, and thus plays an important regulatory role in DN.

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更新日期/Last Update: 2020-10-02