[1]李进,黎敏,鲁卫平.MALDI-TOF MS技术对VIM型和SPM型金属酶铜绿假单胞菌的检测分析[J].第三军医大学学报,2020,42(16):1663-1669.
 LI Jin,LI Min,LU Weiping.Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for detection of VIM- and SPM-type metallo-β-lactamase-producing Pseudomonas aeruginosa[J].J Third Mil Med Univ,2020,42(16):1663-1669.
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MALDI-TOF MS技术对VIM型和SPM型金属酶铜绿假单胞菌的检测分析(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第16期
页码:
1663-1669
栏目:
临床医学
出版日期:
2020-08-30

文章信息/Info

Title:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for detection of VIM- and SPM-type metallo-β-lactamase-producing Pseudomonas aeruginosa
作者:
李进黎敏鲁卫平
陆军军医大学(第三军医大学)大坪医院检验科
Author(s):
LI Jin LI Min LU Weiping

Department of Clinical Laboratory, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China

关键词:
MALDI-TOF MSVIM型和SPM型MBL-PA识别模型性能评价
Keywords:
 
分类号:
R372; R378.991; R446.5
文献标志码:
A
摘要:

目的了解基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF MS)在检测金属酶铜绿假单胞菌(metallo-β-lactamase Pseudomonas aeruginosa, MBL-PA)类型中的作用。方法 收集2015—2018年重庆某三甲医院临床分离的258株碳青霉烯类耐药铜绿假单胞菌进行EDTA协同试验和耐药基因检测,通过建立VIM型和SPM型MBL-PA识别模型数据库,对MALDI-TOF MS检测MBL-PA类型的灵敏度、特异度、阳性预测值和阴性预测值进行分析。结果258株碳青霉烯类耐药铜绿假单胞菌中EDTA表型初筛试验阳性106株,经PCR耐药基因检测得到VIM型40株,SPM型35株。通过MALDI-TOF MS识别模型检测出23/25 VIM型菌株,灵敏度为92.0%,特异性为100.0%,阳性预测值为100.0%,阴性预测值为93.7%。对于SPM型菌株,检测出16/20,灵敏度为80.0%,特异性为100.0%,阳性预测值均为100.0%,阴性预测值为88.2%。结论MALDI-TOF MS在检测MBL-PA方面比常规PCR技术更有优势,通过构建识别模型可快速准确地鉴别VIM型和SPM型MBL-PA,具备非常广的应用前景。 

Abstract:
ObjectiveTo evaluate the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for detecting VIM- and SPM-type metallo-β-lactamase-producing Pseudomonas aeruginosa (MBL-PA). MethodsA total of 258 clinical isolates of carbapenem-resistant P. aeruginosa collected from our hospital from 2015 to 2018 were analyzed using EDTA test and PCR-based drug resistance gene detection. A VIM- and SPM-type recognition model database was established to assess the sensitivity, specificity, positive predictive value and negative predictive value of MALDI-TOF MS for detecting different types of MBL-PA. ResultsEDTA test detected 106 positive strains from the 258 clinical isolates of carbapenem-resistant P. aeruginosa, and PCR-based drug resistance gene detection identified 40 VIM-type MBL-PA strains and 35 SPM-type MBL-PA strains. The MALDI-TOF MS recognition model detected 23 out of 25 VIM-type MBL-PA strains with a sensitivity of 92.0%, a specificity of 100.0%, a positive predictive value of 100.0%, and a negative predictive value of 93.7%, and identified 16 out of 20 SPM-type MBL-PA strains with a sensitivity of 80.0%, a specificity of 100.0%, a positive predictive value of 100.0%, and a negative predictive value of 88.2%. ConclusionMALDI-TOF MS has more advantages than conventional PCR in detecting the types of MBL-PA, and can quickly and accurately identify the VIM- and SPM-type MBL-PA after construction of a recognition model.
 
更新日期/Last Update: 2020-08-17