[1]胡骏豪,武晓静,顾文竹,等.主动脉缩窄致大鼠左心功能不全对肺血管重构的影响及与BMP7表达的关系[J].第三军医大学学报,2020,42(14):1385-1391.
 HU Junhao,WU Xiaojing,GU Wenzhu,et al.Effects of transverse aortic constriction-induced left ventricular dysfunction on pulmonary vascular remodeling in rats and its relationship with bone morphogenetic protein 7 [J].J Third Mil Med Univ,2020,42(14):1385-1391.
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主动脉缩窄致大鼠左心功能不全对肺血管重构的影响及与BMP7表达的关系(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第14期
页码:
1385-1391
栏目:
基础医学
出版日期:
2020-07-30

文章信息/Info

Title:
Effects of transverse aortic constriction-induced left ventricular dysfunction on pulmonary vascular remodeling in rats and its relationship with bone morphogenetic protein 7
 
作者:
胡骏豪武晓静顾文竹靳鹏刘嘉玲周骐
重庆医科大学附属第二医院心血管内科;深圳大学总医院心血管内科 ;陆军军医大学(第三军医大学)第二附属医院心血管内科
Author(s):
HU Junhao WU Xiaojing GU Wenzhu JIN Peng LIU Jialing ZHOU Qi

Department of Cardiology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010; 2Department of Cardiology, Shenzhen University General Hospital, Shenzhen, Guangdong Province, 518055; 3Department of Cardiology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, China

关键词:
主动脉弓缩窄肺血管重构高血压 肺性心功能骨形成蛋白7
Keywords:
transverse aortic constriction pulmonary vascular remodeling hypertension pulmonary cardiac function bone morphogenetic protein 7
分类号:
R322.12; R363.21; R541
文献标志码:
A
摘要:

目的通过大鼠主动脉弓缩窄(transverse aortic constriction,TAC)建立左心功能不全模型,观察对肺血管重构和肺血流动力学的影响及与肺组织骨形成蛋白7(bone morphogenetic protein 7,BMP7)表达的关系。方法16只健康雄性SD 大鼠根据建模分为手术组(TAC组,n=8)和假手术组(Sham组,n=8),TAC组行主动脉弓缩窄术;Sham组仅穿丝线不结扎主动脉弓。术后9周时,采用超声心动图检测大鼠左心结构和功能变化,通过心导管术观察肺血流动力学和右心功能的变化,采用HE、Masson染色观察心肌和肺血管变化,采用RT-qPCR、Western blot分别检测肺组织中BMP7 mRNA和蛋白表达。结果与Sham组相比,术后9周,超声心动图检测发现TAC组大鼠左室后壁和室间隔增厚、左室舒张容积增加(P<0.01),左室射血分数降低(P<0.01);血流动力学检测提示TAC组平均肺动脉压升高(P<0.01),右心室收缩压升高(P<0.01);病理学观察提示TAC组左室心肌细胞横截面积增大(P<0.01),心肌纤维化程度增加(P<0.01),肺小动脉中层增厚程度增加(P<0.01);RT-qPCR和Western blot检测发现TAC组肺组织中BMP7 mRNA和蛋白表达均减少(P<0.05)。结论左心重构和左室功能不全引起肺高压和肺血管重构时,除与血流动力学因素直接有关外,可能还与肺组织BMP7表达减少有关,提示心脏对肺的器官间调节可能参与了左心疾病相关肺高压的形成。

Abstract:

ObjectiveTo study the effects of left ventricular dysfunction induced by transverse aortic constriction (TAC) on pulmonary vascular remodeling and hemodynamics, and the relationship with bone morphogenetic protein 7 (BMP7) expression in the lung tissues of rats. MethodsSixteen healthy male Sprague-Dawley rats were randomized equally into TAC model group and sham operation group. At the end of 9 weeks after the surgery, cardiac remodeling and the changes of cardiac functions were assessed by cardiac ultrasound. The pulmonary hemodynamic parameters and the right ventricular functions were evaluated by cardiac catheterization. HE and Masson staining was used to observe pulmonary vascular remodeling, left ventricular myocardial cell size and left ventricular myocardial cell fibrosis in the rats. The mRNA and protein levels of BMP7 in the lung tissues of the rats were detected using RT-qPCR and Western blotting. ResultsCompared with the sham-operated rats, the rats with TAC showed significantly increased left ventricular wall and septal thickness, increased left ventricular end-diastolic volume (P<0.01), and decreased left ventricular ejection fraction (P<0.01). Hemodynamic analysis showed significant increases of the mean pulmonary arterial pressure (mPAP) and right ventricular systolic pressure (RVSP) in rats with TAC as compared with the sham-operated rats (P<0.01). Pathological examination revealed that in rats with TAC, the cross-sectional area of the left ventricular cardiomyocytes increased significantly (P<0.01) with obvious myocardial fibrosis (P<0.01) and thickening of the medial layer of the pulmonary arterioles (P<0.01) in comparison with the sham-operated group. The expression of BMP7 was decreased significantly at both the mRNA and protein levels in the pulmonary tissue in TAC group as compared with the sham-operated group (P<0.05). ConclusionPulmonary hypertension and pulmonary vascular remodeling caused by left ventricular remodeling and left ventricular dysfunction might be related to decreased pulmonary BMP7 expression in addition to the direct influence by changes in the hemodynamics, suggesting that the organ crosstalk between the heart and the lung might participate in the occurrence of pulmonary hypertension resulting from left heart diseases.
 

参考文献/References:

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更新日期/Last Update: 2020-07-23