[1]杨松巍,王亮,李虎进,等.骨与软组织肉瘤患者检测循环肿瘤细胞及HOXB7基因表达的临床意义[J].第三军医大学学报,2020,42(10):1047-1052.
 YANG Songwei,WANG Liang,LI Hujin,et al.Clinical significance of circulating tumor cells and their homeobox gene B7 expression in patients with bone and soft tissue sarcomas[J].J Third Mil Med Univ,2020,42(10):1047-1052.
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骨与软组织肉瘤患者检测循环肿瘤细胞及HOXB7基因表达的临床意义(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第10期
页码:
1047-1052
栏目:
临床医学
出版日期:
2020-05-30

文章信息/Info

Title:
Clinical significance of circulating tumor cells and their homeobox gene B7 expression in patients with bone and soft tissue sarcomas
作者:
杨松巍王亮李虎进单东力王全陈飚
重庆大学附属肿瘤医院骨与软组织肿瘤科
Author(s):
YANG Songwei WANG Liang LI Hujin SHAN Dongli WANG Quan CHEN Biao

Department of Bone and Soft Tissue Oncology, Chongqing University Cancer Hospital, Chongqing, 400030, China

关键词:
 
Keywords:
bone and soft tissue sarcomas circulating tumor cell homeobox gene B7 epithelialmesenchymal transition prognosis
分类号:
R730.23; R730.43; R738.1
文献标志码:
A
摘要:

目的探讨骨与软组织肉瘤患者外周血中循环肿瘤细胞(circulating tumor cell,CTC)的数量、类型以及循环肿瘤细胞上同源盒基因B7(homeobox genes B7,HOXB7)表达的临床意义。方法回顾性分析本院2017年5月至2019年7月经病理确诊的骨与软组织肉瘤患者40例,患者确诊后均采用CanPatrolTM富集技术检测循环肿瘤细胞,采用多重mRNA原位分析对循环肿瘤细胞进行分型鉴定,并使用RNA探针检测循环肿瘤细胞上HOXB7基因的表达情况。结合临床资料,分析CTC计数、类型及HOXB7基因表达与骨与软组织肿瘤的分期及转移之间的关系。结果40例患者中有37例在治疗前检测出CTC细胞,检测出的CTC分为上皮型、混合型和间质型。CTC计数与骨与软组织肉瘤的分期密切相关(P=0.002),Ennecking Ⅲ期患者外周血CTC计数明显多于Ennecking Ⅱ期(P=0.040);Ennecking Ⅲ期患者间质型CTC的计数也显著高于Ennecking Ⅱ期(P=0.001)。HOXB7在Ennecking Ⅲ期患者CTC中的阳性表达率为56.9%,显著高于其他分期(P=0.003)。对骨肉瘤进行亚组分析,Ennecking Ⅲ期患者CTC中HOXB7表达的阳性率显著高于Ennecking Ⅱ期(P=0.025)。结论骨与软组织肉瘤患者外周血循环肿瘤细胞细胞计数、分型及HOXB7基因的表达与肿瘤分期、转移密切相关。

Abstract:
ObjectiveTo investigate the clinical significance of the count and type of circulating tumor cell (CTC) and the expression of homeobox gene B7 (HOXB7) gene in the CTC in patients with bone and soft tissue sarcomas. MethodsWe retrospectively analyzed the data of 40 patients with newly diagnosed bone and soft tissue sarcomas from May, 2017 to July, 2019 at Chongqing University Cancer Hospital. After the diagnosis, all the patients were examined for peripheral blood CTC using the CanPatrolTM System, and the isolated CTC was characterized using a multiple messenger RNA in situ analysis (MRIA). The expression of HOXB7 gene in the CTC was detected using a RNA probe. The correlations of CTC counts, cell type and the expression level of HOXB7 gene with clinical stages and metastasis of bone and soft tissue sarcomas were analyzed. Resultsof the 40 patients with sarcoma, CTC including epithelial CTC, mesenchymal CTC (MCTC), and biophenotypic epithelial/mesenchymal CTC were detected in 37 patients before treatment. The CTC counts correlated significantly with Enneking stages (P=0.002). The number of CTC and the ratio of mesenchymal CTC were significantly greater in stage Ⅲ patients than in stage Ⅱ patients (P=0.040 and 0.001, respectively). Positive expression of HOXB7 in the CTC was detected in 56.9% of the stage Ⅲ patients, a rate significantly higher than that in patients in other stages (P=0.003). Subgroup analysis of osteosarcoma showed that the positivity rate of HOXB7 in the CDC was significantly higher in stage Ⅲ patients than in stage Ⅱ patients (P=0.025). ConclusionIn patients with bone and soft tissue sarcomas, the counts and types of peripheral blood CTC and HOXB7 expression in the CDC are closely correlated with metastasis and clinical Enneking stages, and can serve as indicators for assessing tumor metastasis, therapeutic effect and prognosis of the patients.
 

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更新日期/Last Update: 2020-05-22