[1]黄瑶,范围,杨梅,等.Angptl2通过PirB激活视网膜Müller细胞参与眼内炎症[J].第三军医大学学报,2020,42(01):39-44.
 HUANG Yao,FAN Wei,YANG Mei,et al.Angptl2 mediates intraocular inflammation by activating Müller cells through PirB[J].J Third Mil Med Univ,2020,42(01):39-44.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
42卷
期数:
2020年第01期
页码:
39-44
栏目:
基础医学
出版日期:
2020-01-15

文章信息/Info

Title:
Angptl2 mediates intraocular inflammation by activating Müller cells through PirB
作者:
黄瑶范围杨梅袁容娣
陆军军医大学(第三军医大学)第二附属医院眼科
Author(s):
HUANG Yao FAN Wei YANG Mei YUAN Rongdi

Department of Ophthalmology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400037, China

关键词:
血管生成素样蛋白2配对免疫球蛋白样受体B视网膜ller细胞炎症
Keywords:
angiopoietin-like protein 2 paired immunoglobulin-like receptor B retinaller cells inflammation
分类号:
R322.91; R363.21; R364.5
文献标志码:
A
摘要:

目的 探讨血管生成素样蛋白2(angiopoietin-like protein 2,Angptl2)在眼内促进炎症的作用和机制。方法实验组大鼠玻璃体腔注射0.2 mg LPS,对照组加入等量PBS,RT-PCR检测两组大鼠视网膜Angptl2的表达。以原代培养的SD大鼠视网膜Müller细胞为研究对象,加入外源性Angptl2后,细胞计数观察Müller细胞增殖变化,免疫荧光观察Müller细胞GFAP的表达以及RT-PCR检测促炎因子IL-1β、IL-6、TNF-α的表达变化。免疫荧光观察配对免疫球蛋白样受体B(paired immunoglobulin-like receptorB,PirB)在Müller细胞上的表达;Müller细胞外源性加入Angptl2后,再分别转染Ad-PirB-siRNA腺病毒载体和腺病毒对照Ad-C,分为PBS对照组、Angptl2组、Ad-C+Angptl2组、Ad-PirB-siRNA+Angptl2组,RT-PCR观察炎症因子的表达变化。结果LPS组Angptl2 mRNA表达较对照组明显增加(P<0.05);Angptl2组较PBS对照组Müller细胞增殖增多,GFAP表达增加,IL-1β、IL-6、TNF-α表达也增加,差异具有统计学意义(P<0.05);Müller细胞表达Angptl2的配体PirB,Ad-PirB-siRNA干扰Müller细胞PirB表达后,Ad-PirB-siRNA+Angptl2组较Angptl2组和Ad-C+Angptl2组炎症因子IL-6、TNF-α降低,差异具有统计学意义(P<0.05)。结论Angptl2通过PirB激活Müller细胞分泌促炎因子IL-1β、IL-6、TNF-α参与眼内炎症。

Abstract:

ObjectiveTo investigate the pro-inflammatory effects and mechanisms of angiopoietin-like protein 2 (Angptl2) in the eyes. MethodsSD rats (8 weeks old) were given a single intravitreal injection of 0.2 mg LPS or PBS to establish intraocular inflammation model or sham operation. Then the Angptl2 expression in the retina was examined by real-time PCR (RT-PCR). Müller cells were isolated from the retina of newborn SD rats (with 24 h after born). After the obtained cells were purified and identified, the recombinant Angptl2 was added to the primarily cultured cells. Cell count, immunofluorescence assay and RT-PCR were used to assess the proliferation of Müller cells, the expression of GFAP, and levels of interleukin-1 beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor (TNF-α), respectively. The expression of Angptl2 ligand, paired immunoglobulin-like receptor B (PirB), in the Müller cells was observed by immunofluorescence assay. RT-PCR was employed to observed the expression of IL-6 and TNF-α in the Müller cells of PBS group, Angptl2 group, Ad-C (transfection of adenovirus negative control Ad-C)+Angptl2 group, and Ad-PirB-siRNA (transfection of adenovirus Ad-PirB-siRNA)+Angptl2 group. ResultsThe mRNA level of Angptl2 was increased notably in the retina of the LPS-treated rats (intraocular inflammation model) than the control rats (P<0.05). Exogenous Angptl2 treatment resulted in higher proliferation rate, expression of GFAP and the levels of IL-1β, IL-6 and TNF-α in Müller cells (P<0.05). PirB was expressed in the retinal Müller cells. Interference of PirB by adenovirus Ad-PirB-siRNA significantly decreased the levels of IL-6 and TNF-α (P<0.05). ConclusionAngptl2 mediates acute intraocular inflammation by activating Müller cells to secrets IL-1β, IL-6 and TNF-α though PirB.

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更新日期/Last Update: 2020-01-07