[1]师培霞,戴琴,任辉.社交挫败模型中应激量的确定以及奖赏在不同应激量下的作用[J].第三军医大学学报,2019,41(20):2023-2028.
 SHI Peixia,DAI Qin,REN Hui.Determination of stress intensity and effect of reward under different stress intensities in a mouse model of social stress[J].J Third Mil Med Univ,2019,41(20):2023-2028.
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社交挫败模型中应激量的确定以及奖赏在不同应激量下的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第20期
页码:
2023-2028
栏目:
医学心理学
出版日期:
2019-10-30

文章信息/Info

Title:
Determination of stress intensity and effect of reward under different stress intensities in a mouse model of social stress
作者:
师培霞戴琴任辉
陆军军医大学护理系护理管理学教研室
Author(s):
SHI Peixia DAI Qin REN Hui

Department of Nursing Management, Army Medical University (Third Military Medical University), Chongqing, 400038, China

关键词:
社交挫败模型应激量奖赏抑郁样行为
Keywords:
social defeat model stress intensity reward depression-like behaviors
分类号:
R395.1;R749.4;R846
文献标志码:
A
摘要:

目的 探讨通过社交挫败应激建立抑郁模型时如何确定应激量的大小,并比较奖赏在不同应激量下对抑郁样行为的作用。方法 将9~12周龄C57BL/6J野生型雄性小鼠按以下3种方法进行社交挫败应激建模:①直接建模,建模完成后比较被攻击强度与抑郁样行为的相关性(n=8);②控制攻击时间,大应激量组每天被攻击10 min(n=6),小应激量组每天被攻击5 min(n=6),对照组没有被攻击(n=6);③控制逃避空间,大应激量组每天在294 mm×190 mm×125 mm的小笼子中被攻击(n=6),小应激量组每天在475 mm×350 mm×200 mm的大笼子中被攻击(n=6),对照组没有被攻击(n=6)。建模完成后,通过社会交互、糖水偏好和强迫游泳等行为学测试比较何种方法更能区分不同应激量造成的抑郁样行为。利用确定应激量的最佳方法进行社交挫败建模,在建模过程中给予奖赏,即每天给予9~12周龄C57BL/6J雌性小鼠陪伴24 h,从而比较奖赏在不同应激量下对抑郁样行为的作用。结果建模完成后,小鼠均表现出不同程度的抑郁样行为。通过控制逃避空间,小应激量组与大应激量组在社会交互区的社会交互指数(P<0.05)、角落区的社会交互指数(P<0.05)以及强迫游泳不动时间上差异无统计学意义(P<0.05),另外两种方法无法很好地区分应激量;在大应激量下,奖赏组显著改变了社会交互区的社会交互指数(P<0.01)、角落区的社会交互指数(P<0.05)和强迫游泳的不动时间(P<0.05);在小应激量下,奖赏组只改变了社会交互区的社会交互指数(P<0.05);在大应激量下,奖赏组与无奖赏组在社会交互区的社会交互指数(P<0.05)、角落区的社会交互指数(P<0.05)和强迫游泳不动时间(P<0.01)的差值更大。结论在社交挫败建模中,通过控制逃避空间能够更好地确定应激量的大小,奖赏可以降低应激造成的抑郁样行为,并在大应激量下发挥的作用更大。

Abstract:

Objective To determine the stress intensity in a mouse model of depression induced by social defeat stress and observe the effects of reward on depression-like behaviors of the mice exposed to different stress intensities. MethodsWe assessed the depression-like behaviors of male wild-type C57BL/6J mice (9-12 weeks old) in response to attacks by CD-1 mice, and investigated the effects of different stress intensities controlled by the attack duration (10 min vs 5 min per day) and the escape space (in a small vs large cage) on depression-like behaviors of the mice to determine the optimal method for modeling. The mouse models of social stress established using the optimal method were given rewards by providing them with company by female C57BL/6J mice (9-12 weeks old) for 24 h during the modeling, and the effects of the reward on depression-like behaviors were evaluated in the mice exposed to different stress intensities. ResultsThe attacks by CD-1 mice induced depression-like behaviors in C57BL/6J mice. Controlling the escape space significantly affected the social interaction indexed in the social interaction zone (P<0.05) and the corner zone (P<0.05) and the forced swimming time of mice (P<0.05), while the other two methods did not well distinguish the effects of different stress intensities. Under a high stress intensity, the mice in the reward group showed significant changes in the social interaction indexes in the social interaction zone (P<0.01) and the corner zone (P<0.05) and the immobility time during forced swimming (P<0.05); under a low stress intensity, the reward only changed the social interaction index in the social interaction zone (P<0.05). The reward-induced changes in the social interaction index in the social interaction zone (P<0.05) and the corner zone (P<0.05) and the immobility time during forced swimming (P<0.01) were significantly more obvious under a high stress intensity than under a low stress intensity. ConclusionIn preparing mouse models of social defeat stress, controlling the escape space allows more efficient determination of the stress intensity. Reward can reduce stress-induced depression-like behaviors in mice, especially when a high stress intensity is applied.

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更新日期/Last Update: 2019-10-25