[1]秦曦,姜政.PYCR1在结肠癌中的表达及对细胞的增殖、侵袭及迁移的影响[J].第三军医大学学报,2019,41(20):1961-1968.
 QIN Xi,JIANG Zheng.Expression of pyrroline-5-carpoxylate reductase-1 in colon cancer and its role in tumor cell proliferation, migration and invasion[J].J Third Mil Med Univ,2019,41(20):1961-1968.
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PYCR1在结肠癌中的表达及对细胞的增殖、侵袭及迁移的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第20期
页码:
1961-1968
栏目:
基础医学
出版日期:
2019-10-30

文章信息/Info

Title:
Expression of pyrroline-5-carpoxylate reductase-1 in colon cancer and its role in tumor cell proliferation, migration and invasion
作者:
秦曦姜政
重庆医科大学附属第一医院消化内科
Author(s):
QIN Xi JIANG Zheng  

Department of Gastroenterology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

关键词:
PYCR1结肠癌侵袭迁移增殖
Keywords:
pyrroline-5-carpoxylate reductase-1 colorectal cancer proliferation migration invasion
分类号:
R361.2;R656.9;R735.3
文献标志码:
A
摘要:

目的 探索PYCR1(pyrroline-5-carpoxylate reductase-1,吡咯啉-5-羧酸还原酶-1)在结肠癌中的表达和对结肠癌细胞的增殖、侵袭和迁移的影响。方法 RT-qPCR和免疫组化检测PYCR1在结肠癌及癌旁组织的表达情况。RT-qPCR和Western blot筛选结肠癌细胞株和检验质粒转染后干扰效果。CCK8、克隆形成、Transwell实验检测增殖、侵袭和迁移能力。Western blot检测上皮间质转化相关蛋白表达情况。结果PYCR1在结肠癌组织中呈高表达(P<0.01),选取HCT116和SW480作为后续实验对象(P<0.01)。构建PYCR1干扰质粒,RT-qPCR和Western blot结果显示shPYCR1组mRNA(P<0.05)和蛋白表达(P<0.01)明显低于shPYCR1-vector组。与shPYCR1-vector组相比,shPYCR1组细胞的增殖、侵袭和迁移能力均减弱(P<0.05)。Western blot结果显示相对于shPYCR1-vector组,shPYCR1组的E-cadherin表达上升(P<0.05),N-cadherin、Vimentin、β-catenin和c-Myc表达下降(P<0.05)。结论 PYCR1在结肠癌组织中呈高表达,干扰PYCR1可明显抑制结肠癌细胞增殖、迁移和侵袭能力。

Abstract:

Objective To investigate the expression of pyrroline-5-carpoxylate reductase-1 (PYCR1) in colon cancer and the effect of PYCR1 silencing on the proliferation, migration and invasion of colon cancer cells in vitro. MethodsWe examined the expression of PYCR1 in clinical specimens of colorectal tissues and adjacent tissues and in 4 colorectal cell lines using RT-qPCR, immunohischemistry or Western blotting. Two colorectal cancer cell lines HCT116 and SW480 were transfected with a recombinant plasmid carrying short hairpin RNA (shRNA) targeting PYCR1, and the changes in the cell proliferation, migration and invasion were detected using CCK8 assay, colony formation assay and Transwell assay. The expression levels of the proteins associated with epithelial-mesenchymal transition (EMT) were also examined in the transfected cells using Western blotting. ResultsPYCR1 was highly expressed in colorectal tissues. PYCR1 silencing mediated by the specific shRNA in the 2 colorectal cancer cell lines significantly suppressed the cell proliferation and lowered the migration and invasion abilities of the cells (P<0.05), resulting in also an increased expression of E-cadherin (P<0.05) but lowered expression of N-cadherin, vimentin, β-catenin and c-Myc (P<0.05). ConclusionPYCR1 is highly expressed in colorectal tissues. Knocking down PYCR1 significantly inhibits the proliferation, migration and invasion of colorectal cancer cells in vitro.

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更新日期/Last Update: 2019-10-25