[1]蔡娇,李甫,段江洁,等.人乙醛脱氢酶家族1成员A3调控胰腺癌细胞的侵袭能力[J].第三军医大学学报,2019,41(17):1642-1648.
 CAI Jiao,LI Fu,et al.ALDH1A3 regulates invasion ability of pancreatic cancer cells[J].J Third Mil Med Univ,2019,41(17):1642-1648.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第17期
页码:
1642-1648
栏目:
基础医学
出版日期:
2019-09-15

文章信息/Info

Title:
ALDH1A3 regulates invasion ability of pancreatic cancer cells
作者:
蔡娇李甫段江洁余时沧
400038 重庆,陆军军医大学(第三军医大学) 第一附属医院:全军临床病理学研究所,西南癌症中心1,干细胞与再生医学科2,肿瘤科3
Author(s):
CAI Jiao1 2 LI Fu3 DUAN Jiangjie1 2 YU Shicang1 2

1Institute of Pathology, Southwest Cancer Center, 2Department of Stem Cell and Regenerative Medicine, 3Department of Oncology, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, China

关键词:
乙醛脱氢酶家族1成员A3胰腺癌肿瘤侵袭JAK/STAT3通路
Keywords:
aldehyde dehydrogenase 1 family member A3 pancreatic cancer neoplasm invasiveness JAK/STAT3 signaling pathway
分类号:
R345; R730.23; R735.9
文献标志码:
A
摘要:

目的探讨人乙醛脱氢酶家族1成员A3(aldehyde dehydrogenase 1 family member A3,ALDH1A3)在胰腺癌及正常胰腺组织中的表达情况,并研究其对胰腺癌侵袭的调控及可能的机制。方法利用癌症和肿瘤基因图谱(the Cancer Genome Atlas,TCGA)数据库中胰腺癌患者数据集、基因组织表达项目(GenotypeTissue Expression Project,GTEx)中正常胰腺组织基因表达谱数据和肿瘤细胞系百科全书(Cancer Cell Line Encyclopedia,CCLE)数据库中胰腺癌细胞系的基因表达谱矩阵,分析ALDH家族各亚型的表达情况;Transwell实验检测敲低ALDH1A3对胰腺癌细胞侵袭能力的影响;基因富集分析(gene set enrichment analysis,GSEA) 探讨ALDH1A3与JAK/STAT3通路活化的关系。结果ALDH1A3在胰腺癌组织中的表达高于正常胰腺组织;在胰腺癌细胞系中的表达高于其他ALDH亚型;下调ALDH1A3可以降低胰腺癌细胞系的侵袭能力;JAK/STAT3通路在ALDH1A3高表达胰腺癌患者中显著富集;下调ALDH1A3可抑制STAT3的磷酸化。结论ALDH1A3参与调控胰腺癌细胞的侵袭,其机制可能与JAK/STAT3通路的活化有关。

Abstract:

ObjectiveTo detect the expression level of aldehyde dehydrogenase 1 family member A3 (ALDH1A3) in pancreatic cancer and adjacent tissues, and to study its specific mechanism involved in the regulation of invasion of pancreatic cancer cells. MethodsThe ALDH family expression pattern in pancreatic cancer patients and normal pancreatic tissues was analyzed using the gene expression profile matrix of the Cancer Cell Line Encyclopedia (CCLE) database, the Cancer Genome Atlas (TCGA) database and GenotypeTissue Expression Project (GTEx). Transwell assay was used to detect the invasion of pancreatic cancer cells with silencing of ALDH1A3. Gene set enrichment analysis (GSEA) was employed to further explore the relationship between ALDH1A3 and activation of JAK/STAT3 signaling pathway. ResultsThe expression level of ALDH1A3 was significantly higher in pancreatic cancer tissues than adjacent normal tissues, and it was of the highest level when compared with the other subtypes in pancreatic cancer cell lines. Downregulation of ALDH1A3 resulted in the decreased invasion ability of pancreatic cancer cell lines. JAK/STAT3 pathway was significantly enriched in the pancreatic cancer patients with higher expression of ALDH1A3. The silencing of ALDH1A3 induced the decrease of phosphoSTAT3. ConclusionALDH1A3 may be involved in the regulation of pancreatic cancer cell invasion through the JAK/STAT3 pathway.

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更新日期/Last Update: 2019-09-09