[1]宣晓梅,陈立荣,林琳,等.尖锐湿疣患者外周血淋巴细胞Foxp3与树突状细胞TLR9、IRF-7水平变化特征[J].第三军医大学学报,2019,41(20):2012-2016.
 XUAN Xiaomei,CHEN Lirong,LIN Lin,et al.Correlation of Foxp3 expression in peripheral blood lymphocytes with TLR9 and IRF-7 in dendritic cells in patients with condyloma acuminatum[J].J Third Mil Med Univ,2019,41(20):2012-2016.
点击复制

尖锐湿疣患者外周血淋巴细胞Foxp3与树突状细胞TLR9、IRF-7水平变化特征(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第20期
页码:
2012-2016
栏目:
临床医学
出版日期:
2019-10-30

文章信息/Info

Title:
Correlation of Foxp3 expression in peripheral blood lymphocytes with TLR9 and IRF-7 in dendritic cells in patients with condyloma acuminatum
作者:
宣晓梅陈立荣林琳石丽平董伟兰
河北医科大学第一医院皮肤美容科
Author(s):
XUAN Xiaomei CHEN Lirong LIN Lin SHI Liping DONG Weilan

Department of Cosmetology, First Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, 050031, China

关键词:
转录因子叉头蛋白干扰素调节因子Toll样受体9树突细胞尖锐湿疣
Keywords:
orkhead box protein interferon regulatory factor Toll-like receptor 9 dendritic cells condyloma acuminatum
分类号:
R392;R512.99;R752.5
文献标志码:
A
摘要:

目的 探讨尖锐湿疣患者外周血淋巴细胞(peripheral blood lymphocyte,PBLC)的转录因子叉头蛋白 (forkheadbox protein 3, FOXP3)与树突状细胞(dendritic cells, DC)的 Toll样受体9(Toll-like receptor 9, TLR9)、干扰素调节因子(interferon regulation factor, IRF-7)水平的变化特征。方法 选取本院2017年2月至2018年2月收治的120例尖锐湿疣患者为疾病组,另选取同期健康体检患者120例作为健康组,比较两组患者的DC的TLR9、IRF-7水平、PBLC的Foxp3水平,以及PBLC的Foxp3与DC的TLR9、IRF-7水平的特征。结果 疾病组患者DC的TLR9、IRF-7水平显著高于对照组,疾病组复发患者DC的TLR9、IRF-7水平显著高于初发患者;疾病组PBLC的Foxp3 mRNA、PD-1、PD-L1、CD8+水平高于健康组,疾病组PBLC的RORγt mRNA、CD4+水平低于健康组,且疾病组中,复发患者的Foxp3 mRNA、PD-1、PD-L1、CD8+水平高于初发患者,RORγt mRNA、CD4+水平低于健康组,患者PBLC的Foxp3与DC的TLR9、IRF-7的变化存在正相关关系。结论 尖锐湿疣患者外周血淋巴细胞Foxp3与树突状细胞TLR9、IRF-7变化有关,可能共同作为刺激分子参与疾病的进展。

Abstract:

Objective To analyze the correlation between forkhead box protein 3 (Foxp3) in peripheral blood lymphocytes (PBLCs) and the expression levels of Toll-like receptor 9 (TLR9) and interferon regulatory factor-7 (IRF-7) in the dendritic cells (DCs) in patients with condyloma acuminatum. MethodsFrom February, 2017 to February, 2018, a total of 120 patients with condyloma acuminatum were enrolled as the case group, with 120 healthy subjects undergoing routine physical examination during the same period as the control group. The expression levels of TLR9 and IRF-7 in the DCs and Foxp3 expression levels in the PBLCs were compared between the 2 groups, and the correlation between Foxp3 in the PBLCs and TLR9 and IRF-7 expression in the DCs was analyzed. ResultsTLR9 and IRF-7 levels in the DCs were significantly higher in the case group than in the healthy control group, and their levels were significantly higher in the patients with disease relapse than those with the first-episode disease. The levels of Foxp3, PD-1, PD-L1 and CD8 in the PBLCs were significantly higher, while the levels of RORγt mRNA and CD4 in the PBLCs were significantly lower in the case group than the healthy control group. In the case group, the levels of Foxp3, PD-1, PD-L1 and CD8 were significantly higher in the relapse group than in the first-episode disease group; The levels of RORγt mRNA and CD4 were significantly lower in the relapse group than the control group. In the patients with condyloma acuminatum, Foxp3 level in the PBLCs was significantly correlated with the levels of TLR9 and IRF-7 in the DCs. ConclusionIn patients with condyloma acuminatum, Foxp3 level in the PBLCs is correlated with the changes in TLR9 and IRF-7 levels in the DCs, suggesting their roles all as stimulatory molecules that participate in the disease progression.

参考文献/References:

[1]曹永浩, 张仕坚, 周元聪, 等. 干扰素调节因子家族和免疫调控[J]. 生命的化学, 2006, 26(5): 387-389. DOI: 10.3969/j.issn.1000-1336.2006.05.001.
CAO Y H, ZHANG S J, ZHOU Y C, et al. IRF family and immune regulation[J]. Chemistry of Life, 2006, 26(5): 387-389. DOI: 10.3969/j.issn.1000-1336.2006.05.001.
[2]武乃倩, 田秀芳. 干扰素调节因子4在Toll样受体3介导的小鼠流产过程中的作用及机制[J]. 中国妇幼保健, 2018, 33(10): 2344-2348. DOI: 10.7620/zgfybj.j.issn.1001-4411.2018.10.58.
WU N Q, TIAN X F.Role and mechanism of interferon regulatory factor 4 in Toll-like receptor 3 mediated mouse abortion[J]. Maternal and Child Health Care of China, 2018, 33(10): 2344-2348. DOI: 10.7620/zgfybj.j.issn.1001-4411.2018.10.58.
[3]李晔, 李斌, 宁玻, 等. 尖锐湿疣患者人乳头状瘤病毒感染状况及免疫功能分析[J]. 中华医院感染学杂志, 2016, 26(14): 3299-3301. DOI: 10.11816/cn.ni.2016-153918.
LI Y, LI B, NING B, et al. Status and immune function in patients with genital warts HPV infection[J]. Chin J Nosocomiol, 2016, 26(14): 3299-3301. DOI: 10.11816/cn.ni.2016-153918.
[4]胡雅玉, 胡恩平, 任小丽, 等. 尖锐湿疣感染患者不同病程T淋巴细胞亚群的表达与人乳头状瘤病毒感染的相关性研究[J]. 中华医院感染学杂志, 2016, 26(10): 2227-2228,2241. DOI: 10.11816/cn.ni.2016-154144.
HU Y Y, HU E P, REN X L, et al. Correlation between expression of T lymphocyte subsets of condyloma acuminate patients at different courses of diseases and human papillomavirus infection[J]. Chin J Nosocomiol, 2016, 26(10): 2227-2228, 2241. DOI:10.11816/cn.ni.2016-154144.
[5]陈敏, 何燕燕, 陈云芳, 等. 激光联合重组人干扰素α-2b凝胶治疗尖锐湿疣的临床研究[J]. 中国性科学, 2016, 25(6): 90-92. DOI:10.3969/j.issn.1672-1993.2016.06.029.
CHEN M, HE Y Y, CHEN Y F, et al. Clinical study of laser combined with recombinant human α-2b interferon gel in the treatment of condyloma acuminatum[J]. Chin J Human Sexuality, 2016, 25(6): 90-92. DOI: 10.3969/j.issn.1672-1993.2016.06.029.
[6]中华医学会皮肤性病学分会性病学组,中国医师协会皮肤科分会性病亚专业委员会. 尖锐湿疣诊疗指南(2014)[J]. 中华皮肤科杂志, 2014, 47(8): 598-599.
Department of Sexually Transmitted Diseases, Chinese Society of Dermatology, Department of Dermatology, Chinese Medical Association, Sexually Transmitted Diseases Subcommittee.Condyloma acuminata treatment guide(2014)[J]. Chin J Dermatol, 2014, 47(8): 598-599.
[7]邓国辉, 胡永轩, 吴晓燕, 等. 尖锐湿疣患者外生殖器官微生态与HPV病毒亚型的关系[J]. 牡丹江医学院学报, 2016, 37(3): 13-15.
DENG G H, HU Y X, WU X Y, et al. Relationship between HPV virus subtypes of patients with genital warts and microecological[J]. J Mudanjiang Med Univ, 2016, 37(3): 13-15.
[8]卢淑娇, 吴勤丽, 宁海明, 等. 朗格汉斯细胞与尖锐湿疣的发病与复发机制的探讨[J]. 中国性科学, 2016, 25(11): 75-78. DOI: 10.3969/j.issn.1672-1993.2016.11.025.
LU S J, WU Q L, NING H M, et al. Expression and mechanisms of Langerhans cells in condyloma accuminatum tissues[J]. Chin J Human Sexuality, 2016, 25(11): 75-78. DOI: 10.3969/j.issn.1672-1993.2016.11.025.
[9]CHETTY R. 70 years of the JCP-highly cited papers: The causal relation between human papillomavirus and cervical cancer[J]. J Clin Pathol, 2017, 70(12): 997. DOI:10.1136/jclinpath-2017-204867.
[10]FAZENDIN E A, CREAN A J, FAZENDIN J M, et al. Condyloma acuminatum, anal intraepithelial neoplasia, and anal cancer in the setting of HIV: do we really understand the risk?[J]. Dis Colon Rectum, 2017, 60(10): 1078-1082. DOI: 10.1097/DCR.0000000000000890.
[11]GOODMAN A, PATEL S P, KURZROCK R. PD-1-PD-L1 immune-checkpoint blockade in B-cell lymphomas[J]. Nat Rev Clin Oncol, 2017, 14(4): 203-220. DOI: 10.1038/nrclinonc.2016.168.
[12]BYLICKI O, PALEIRON N, MARGERY J, et al. Targeting the PD-1/PD-L1 immune checkpoint in EGFR-mutated or ALK-translocated non-small-cell lung cancer[J]. Target Oncol, 2017, 12(5): 563-569. DOI: 10.1007/s11523-017-0510-9.
[13]NA K, SUNG J Y, KIM H S.Clinicopathological characteristics of high-grade squamous intraepithelial lesions involving condyloma acuminatum[J]. Anticancer Res, 2018, 38(3): 1767-1774. DOI: 10.21873/anticanres.12414.
[14]OTH T, HABETS T H P M, GERMERAAD W T V, et al. Pathogen recognition by NK cells amplifies the pro-inflammatory cytokine production of monocyte-derived DC via IFN-Γ[J]. BMC Immunol, 2018, 19(1): 8. DOI: 10.1186/s12865-018-0247-y.
[15]NAMBIRAJAN A, SHUKLA A K, MATHUR S R, et al.Condyloma acuminatum of urinary bladder in a male renal transplant recipient - A diagnostic and therapeutic challenge[J]. Clin Genitourin Cancer, 2017, 15(4): e739-e742. DOI: 10.1016/j.clgc.2016.12.007.
 

相似文献/References:

[1]刘凯军,李晋涛,魏昆,等.轮状病毒非结构蛋白1与种属限制性的相关性研究[J].第三军医大学学报,2009,31(03):194.
 LIU Kai-jun,LI Jin-tao,WEI Kun,et al.Correlation between rotavirus NSP1 and species restriction: a bioinformation study[J].J Third Mil Med Univ,2009,31(20):194.

更新日期/Last Update: 2019-10-25