[1]郝军舰,郝丽娟.miR-204-5p通过靶向HMGB1抑制甲状腺乳头状癌K1细胞增殖、侵袭和迁移[J].第三军医大学学报,2019,41(16):1559-1565.
 HAO Junjian,HAO Lijuan.MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene[J].J Third Mil Med Univ,2019,41(16):1559-1565.
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miR-204-5p通过靶向HMGB1抑制甲状腺乳头状癌K1细胞增殖、侵袭和迁移(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第16期
页码:
1559-1565
栏目:
基础医学
出版日期:
2019-08-30

文章信息/Info

Title:
MiR-204-5p inhibits proliferation, invasion and migration of thyroid papillary carcinoma K1 cells in vitro by targeting HMGB1 gene
作者:
郝军舰郝丽娟
青海红十字医院急诊外科
Author(s):
HAO Junjian HAO Lijuan

Department of Emergency Surgery, Qinghai Red Cross Hospital, Xining, Qinghai Province, 810000, China

关键词:
miR-204-5p甲状腺乳头状癌细胞增殖侵袭迁移HMGB1
Keywords:
miR-204-5p papillary thyroid cancer cell proliferation invasion migration HMGB1
分类号:
R394.2;R730.23;R736.1
文献标志码:
A
摘要:

目的 探讨miR-204-5p对甲状腺乳头状癌K1细胞增殖、侵袭和迁移的影响及其机制。方法 将体外培养的K1细胞分为miR-NC组(转染miR-204-5p mimics阴性对照)、miR-204-5p组(转染miR-204-5p mimics)、anti-miR-NC组(转染miR-204-5p inhibitor阴性对照)和anti-miR-204-5p组(转染miR-204-5p inhibitor),RT-PCR检测各组细胞中miR-204-5p的表达,MTT法检测细胞增殖活力,Transwell小室实验检测细胞侵袭和迁移能力。双荧光素酶报告基因实验检测miR-204-5p与HMGB1的靶向关系,RT-PCR和Western blot检测miR-204-5p对HMGB1 mRNA和蛋白表达的调控作用。通过转染HMGB1干扰序列siRNA-HMGB1和HMGB1过表达质粒pcDNA3.1-HMGB1构建HMGB1低表达和过表达的K1细胞株,观察HMGB1对K1细胞增殖、侵袭和迁移的影响。结果 与miR-NC组相比,miR-204-5p组细胞中miR-204-5p表达水平明显升高;而anti-miR-204-5p组细胞中miR-204-5p表达的水平较anti-miR-NC组明显降低(P<0.05)。miR-204-5p组细胞的增殖活力、侵袭能力和迁移能力较miR-NC组均明显降低,而anti-miR-204-5p组较anti-miR-NC组均明显升高(P<0.05);HMGB1是miR-204-5p潜在的靶基因,miR-204-5p可负向调控HMGB1表达。HMGB1低表达可抑制K1细胞增殖、侵袭和迁移,而HMGB1过表达则促进K1细胞增殖、侵袭和迁移。结论miR-204-5p可靶向HMGB1抑制K1细胞增殖、侵袭和迁移。

Abstract:

Objective To investigate the effects of miR-204-5p on the proliferation, invasion and migration of thyroid papillary carcinoma K1 cells and its mechanism. MethodsK1 cells cultured in vitro were transfected with a miR-204-5p mimics, a miR-204-5p inhibitor, or their respective negative controls (miR-NC and anti-miR-NC), and the expression of miR-204-5p in the cells was detected using RT-PCR. The changes in cell proliferation were assessed with MTT assay, and the changes in cell invasion and migration were evaluated with Transwell assay. The targeting relationship between miR-204-5p and HMGB1 gene was tested using dual luciferase reporter gene assay, and the regulation of miR-204-5p on the expressions of HMGB1 mRNA and protein was examined using RT-PCR and Western blotting. The changes in the proliferation, invasion and migration of K1 cells were observed after transfection of the cells with siRNA-HMGB1 or a HMGB1 overexpression plasmid. ResultsThe expression level of miR-204-5p was significantly increased in the cells transfected with miR-204-5p mimics and significantly lowered in cells transfected with miR-204-5p inhibitor as compared with their respective controls (P<0.05). Overexpression of miR-204-5p significantly attenuated while inhibition of miR-204-5p significantly enhanced the proliferation, invasion and migration abilities of the cells in comparison with their respective controls (P<0.05). HMGB1 was a potential target gene of miR-204-5p, and was negatively regulated by miR-204-5p. RNA interference of HMGB1 obviously inhibited K1 cell proliferation, invasion and migration, while overexpression of HMGB1 promoted the proliferation, invasion and migration of the cells. ConclusionOverexpression of miR-204-5p can inhibit the proliferation, invasion and migration of K1 cells by targeted down-regulation of HMGB1 expression.

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更新日期/Last Update: 2019-08-22