[1]李芳芳,谢杨丽,黄俊兰,等.小鼠出生后半月板的发育过程及成纤维细胞生长因子受体的表达模式[J].第三军医大学学报,2019,41(07):646-652.
 LI Fangfang,XIE Yangli,HUANG Junlan,et al.Developmental process and expression patterns of fibroblast growth factor receptors in murine meniscus after birth [J].J Third Mil Med Univ,2019,41(07):646-652.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第07期
页码:
646-652
栏目:
基础医学
出版日期:
2019-04-15

文章信息/Info

Title:
Developmental process and expression patterns of fibroblast growth factor receptors in murine meniscus after birth
 
作者:
李芳芳谢杨丽黄俊兰张若彬蒋宛凌张大力许萌杜晓兰陈林
陆军军医大学(第三军医大学)大坪医院野战外科研究所战创伤康复医学研究室,全军军事训练伤防治与康复实验室, 创伤、烧伤与复合伤国家重点实验室
Author(s):
LI Fangfang XIE Yangli HUANG Junlan ZHANG Ruobin JIANG Wanling ZHANG Dali XU Meng DU Xiaolan CHEN Lin

State Key Laboratory of Trauma, Burns and Combined Injury,  Laboratory for Rehabilitation of Traumatic Injuries, Laboratory for Prevention and Rehabilitation of Military Training Related Injury, Institute of Surgery Research, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China

 

关键词:
半月板发育成纤维细胞生长因子受体小鼠  
Keywords:
meniscus development fibroblast growth factor receptors mice  
分类号:
R322.72; R329.24
文献标志码:
A
摘要:

目的 观察小鼠出生后半月板发育过程的组织形态学变化及3种成纤维细胞生长因子受体(fibroblast growth factor receptors,FGFRs)的时空表达规律。方法 取材出生后不同年龄(1日龄和1、2、4、6、12周龄)C57BL/6J小鼠膝关节行石蜡切片,藏红固绿染色观察小鼠半月板的结构,用免疫组织化学方法检测小鼠半月板Ⅰ型胶原(type Ⅰ collagen, Col Ⅰ)、Ⅱ型胶原(type Ⅱ collagen, Col Ⅱ)和FGFR1、2、3的表达。结果 小鼠出生后,半月板具有明确的内外侧分区,内侧区富含蛋白聚糖,藏红着色较深,而外侧区藏红着色浅。1周时分区更加明显,内侧区主要表达Ⅱ型胶原,而外侧区主要表达Ⅰ型胶原。出生后2周,半月板中间部分细胞开始肥大化,4周时细胞肥大化进一步加重,到6周时,在半月板前角出现小面积的矿化。12周时,半月板出生后的发育过程基本完成,前角出现类似骨髓腔的结构。整个发育过程,FGFR1、2、3的时空表达模式相似,表现为前角的表达于1~2周时达到高峰,而后逐渐减少(P<0.05),并由内侧区均匀分布逐渐变为表层边缘分布,而后角FGFRs的表达无明显变化。结论 小鼠半月板在出生后的发育过程中,软骨基质、胶原分布以及FGFRs的表达均存在时空特异性规律,提示FGFRs在半月板发育过程中起重要作用。
 

Abstract:

Objective To observe the developmental process of murine meniscus after birth and investigate the expression patterns of 3 types of fibroblast growth factor receptors (FGFRs) during this process. Methods Mice at different ages (1 d old and 1, 2, 4, 6 and 12 weeks old) were sacrificed and the knee joints were harvested and embedded in paraffin. The sagittal sections of knee joint were stained with Safranin O-fast green to observe the structure of murine meniscus. The expression of type Ⅰ collagen, type Ⅱ collagen, and 3 types of FGFRs were detected by immunohistochemistry. Results At birth, the meniscus exhibited distinguishable inner and outer regions, with  proteoglycan of darker staining rich in the inner region, while the outer region lightly stained. At 1-week age, the inner-outer region was more obvious, and the inner region mainly expressed type Ⅱ   collagen, while the outer region expressed type Ⅰ collagen. At 2 weeks, some cells in the anterior horn became hypertrophied, and the hypertrophy was further aggravated at 4 weeks of age. By 6 weeks, ossification tissues were observed in the anterior horn of the meniscus. At 12 weeks, the meniscal developmental process after birth was basically completed, and mineralized structure resembling bone marrow cavity was found in the anterior horn. The expression of the 3 types of FGFRs had similar spatiotemporal pattern which reached a peak at 1~2 weeks of age and then gradually decreased (P<0.05) with its spatial distribution changing from a uniform distribution to an edged distribution in the inner zone of anterior horn. However, the expression of FGFRs in the posterior horn had no obvious changes. Conclusion During the postnatal development of murine meniscus, the cartilage matrix, collagen distribution and expression patterns of 3 types of FGFRs have spatiotemporal specificity, which suggest that FGFRs play an important role in the meniscal development.
 

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更新日期/Last Update: 2019-04-05