[1]刘陕西,叶钧,尚杨杨,等.GCNT3在结直肠癌中的表达及临床意义[J].第三军医大学学报,2019,41(10):913-917.
 LIU Shanxi,YE Jun,SHANG Yangyang,et al.Expression and Clinical Significance of GCNT3 in colorectal cancer[J].J Third Mil Med Univ,2019,41(10):913-917.
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GCNT3在结直肠癌中的表达及临床意义(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第10期
页码:
913-917
栏目:
基础医学
出版日期:
2019-05-30

文章信息/Info

Title:
Expression and Clinical Significance of GCNT3 in colorectal cancer
作者:
刘陕西叶钧尚杨杨陈颢元汪荣泉
陆军军医大学西南医院全军消化病研究所
Author(s):
LIU Shanxi YE Jun SHANG Yangyang CHEN Haoyuan WANG Rongquan

Institute of Gastroenterology, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038,China

关键词:
结直肠癌癌症和肿瘤基因数据库GCNT3无病生存率
Keywords:
colorectal cancer Cancer Genome Atlas database GCNT3 gene disease-free survival
分类号:
R574.6;R735.3
文献标志码:
A
摘要:

目的 探讨GCNT3在结直肠癌中表达的水平变化及其对结直肠癌患者预后的影响。方法 国际癌症和肿瘤基因数据库(TCGA)中下载结直肠癌患者GCNT3基因(编码C2GnT-2)的mRNA表达数据及临床信息,整理分析其表达水平变化对结直肠癌临床相关性及预后的影响,并通过细胞增殖实验观察干扰C2GnT-2合成后结直肠癌细胞增殖能力的变化情况。结果 GCNT3在癌组织中较癌旁组织低表达(P<0.001);GCNT3低表达组病人无病生存率显著低于高表达组(P<0.05);干扰C2GnT-2合成后结直肠癌细胞增殖能力显著增强(P<0.01)。结论 GCNT3在结直肠癌中低表达,其低表达可能提示直肠癌患者无病生存率低。

Abstract:

Objective To investigate the expression level of GCNT3, a glycosyltransferase enzyme, in colorectal cancer (CRC) and its association with the prognosis of the patients. Methods The mRNA expression data of GCNT3 gene (encoding mucin-type core 2 synthase enzyme, C2GnT-2) and clinical information of the patients were obtained from the Cancer Genome Atlas database (TCGA). The correlation of GCNT3 mRNA expression level with the clinical characteristics of the patients was analyzed. Kaplan-Meier analysis and Cox regression analysis were used for assessing the prognostic value of GCNT3. We also tested the effect of a short hairpin RNA (shRNA) targeting GCNT3 on the proliferation of the colorectal cancer cell line HT-29 using MTT assay. Results GCNT3 mRNA expression level was significantly decreased in CRC tissues as compared with the paired adjacent colorectal tissues (P<0.001). A low expression level of GCNT3 was significantly correlated with a reduced disease-free survival (DFS) rate in the patients with CRC (P<0.05). In HT-29 cells, interference of C2GnT-2 with the specific shRNA significantly promoted the cell proliferation as compared with the control cells (P<0.01). Conclusion The expression of GCNT3 is significantly downregulated in CRC tissues, and a low expression of GCNT3 can be indicative of a low disease-free survival rate of patients with CRC

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更新日期/Last Update: 2019-05-24