[1]王瑜,张定林,冯伟,等.小鼠铜绿假单胞菌气管插管滴注法肺部感染模型的建立及评价[J].第三军医大学学报,2019,41(10):918-922.
 WANG Yu,ZHANG Dinglin,FENG Wei,et al.Establishment and evaluation of a mouse model of pulmonary Pseudomonas aeruginosa infection by intratracheal instillation[J].J Third Mil Med Univ,2019,41(10):918-922.
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小鼠铜绿假单胞菌气管插管滴注法肺部感染模型的建立及评价(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第10期
页码:
918-922
栏目:
基础医学
出版日期:
2019-05-30

文章信息/Info

Title:
Establishment and evaluation of a mouse model of pulmonary Pseudomonas aeruginosa infection by intratracheal instillation
作者:
王瑜张定林冯伟程林袁慊李晓宇孙凤军
陆军军医大学(第三军医大学):第一附属医院药学部,基础部化学教研室;解放军980医院邯郸分院药剂科
 
Author(s):
WANG Yu ZHANG Dinglin FENG Wei CHENG Lin YUAN Qian LI Xiaoyu SUN Fengjun

Department of Pharmacy, First Affiliated Hospital, Department of Chemistry, College of Basic Medical Sciences,  Army Medical University (Third Military Medical University), Chongqing, 400038;  Department of Pharmacy, Handan Branch of No. 980 Hospital of PLA, Handan, Hebei Province, 056000, China
 

关键词:
铜绿假单胞菌肺部感染气管插管感染模型
Keywords:
Pseudomonas aeruginosa pulmonary infections intratracheal intubation infection model
分类号:
R378;R459.7;R563.1
文献标志码:
A
摘要:

目的 建立小鼠铜绿假单胞菌(Pseudomonas aeruginosa, PA)肺部感染模型,并对该模型进行初步评价。方法 采用一种快速无创的小鼠气管插管滴注法,将小鼠在透射灯照射下经口咽气管插管,滴注1×108 CFU/mL的铜绿假单胞菌悬液50 μL,待接种感染7 d后处死,进行肺部菌落计数和病理学评价。结果 经气管接种感染后,与对照组小鼠比较,感染组小鼠生理状态不佳,肺组织匀浆检出PA,血液中白细胞和中性粒细胞数目显著增多(P<0.05),且出现肺部病理学改变,显示有炎症反应;对照组小鼠肺组织匀浆则未见PA生长,且血液中白细胞和中性粒细胞数目正常,未出现炎症反应。结论 采用小鼠气管插管滴注法,通过呼吸道感染途径可成功建立小鼠肺部PA感染模型,该模型具有稳定性好,易定量的优点

Abstract:

Objective To establish and validate a mouse model of pulmonary infection of Pseudomonas aeruginosa.  Methods The models of pulmonary Pseudomonas aeruginosa infection was established in mice by intratracheal instillation of 50 μL (1×108 CFU/mL) Pseudomonas aeruginosa suspension delivered through orotracheal intubation under a transillumination lamp. At 7 d after the infection, the mice were sacrificed for counting the bacterial colonies in the lungs and for pathological evaluation. Results After intratracheal instillation of  Pseudomonas aeruginosa, the mice showed a poor general condition and had positive findings of Pseudomonas aeruginosa in the lung tissues with significantly increased blood white blood cell and neutrophil counts compared with the control mice (P<0.05); pathological examination revealed obvious pathological changes and inflammations in the lungs of the mice after the infection. In the control mice, no bacteria or pathological changes were detected in the lung tissue, and the white blood cell and neutrophil counts were normal without evidence of inflammatory responses. Conclusion We successfully established a mouse model of pulmonary Pseudomonas aeruginosa infection by delivering the pathogens through tracheal intubation. This model shows a good stability and allows convenient quantitative analysis.
 

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更新日期/Last Update: 2019-05-24