[1]郭晓平,向颖,袁帅,等.非小细胞肺癌患者外周血中lncRNA NEAT1的表达及诊断意义[J].第三军医大学学报,2019,41(05):448-453.
 GUO Xiaoping,XIANG Ying,YUAN Shuai,et al.Expression and diagnostic significance of lncRNA NEAT1 in peripheral blood of patients with non-small cell lung cancer[J].J Third Mil Med Univ,2019,41(05):448-453.
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非小细胞肺癌患者外周血中lncRNA NEAT1的表达及诊断意义
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第05期
页码:
448-453
栏目:
临床医学
出版日期:
2019-03-15

文章信息/Info

Title:
Expression and diagnostic significance of lncRNA NEAT1 in peripheral blood of patients with non-small cell lung cancer
作者:
郭晓平向颖袁帅吴龙邬娜李成英许斌余祖滨白莉杨敬源李亚斐
贵州医科大学公共卫生学院流行病学教研室;陆军军医大学(第三军医大学)军事预防医学系军队流行病学教研室;陆军军医大学(第三军医大学)第二附属医院:胸外科,全军呼吸内科研究所,全军呼吸病研究重点实验室
Author(s):
GUO Xiaoping XIANG Ying YUAN Shuai WU Long WU Na LI Chengying XU Bin YU Zubin BAI Li YANG Jingyuan LI Yafei

Department of Epidemiology, School of Public Health, Guizhou Medical University, Guiyang, Guizhou Province, 550000; Department of Epidemiology, Faculty of Military Preventive Medicine, Army Medical University(Third Military Medical University), Chongqing, 400038; Department of Thoracic Surgery, Institute of Respiratory Diseases, Key Laboratory of Respiratory Diseases, Second Affiliated Hospital, Army Medical University(Third Military Medical University), Chongqing, 400037, China

关键词:
 
Keywords:
non-small cell lung cancerlong non-coding RNANEAT1plasmadiagnosis
分类号:
R342.2; R730.43; R734.2
文献标志码:
A
摘要:

目的 探究肺癌患者血浆中lncRNA NEAT1的表达水平以及对非小细胞肺癌的诊断价值。方法 采用qRT-PCR技术检测258例非小细胞肺癌患者、115例肺部良性病变患者及70例健康对照外周血血浆样本中NEAT1的表达水平,比较其在血浆中的差异表达;分析NEAT1表达水平与肺癌患者临床特征的相关性;绘制ROC曲线分析血浆NEAT1水平对非小细胞肺癌的诊断价值。结果 非小细胞肺癌患者血浆NEAT1水平较对照样本高表达(P<0.01);血浆NEAT1水平与非小细胞肺癌患者年龄、肿瘤大小相关,在>60岁组及肿瘤大小>3 cm组高表达,差异具有统计学意义(P<0.05);ROC曲线结果显示血浆NEAT1水平对区分非小细胞肺癌、早期非小细胞肺癌(Ⅰ期+Ⅱ期)、肿瘤大小≤3 cm的非小细胞肺癌与对照均有诊断价值,ROC曲线下面积分别为0.72、0.73、0.67。血浆NEAT1水平对于区分肿瘤大小≤3 cm的非小细胞肺癌与对照具有较高的灵敏度(93%)和阴性预测值(94%)。结论 NEAT1在非小细胞肺癌血浆中高表达,NEAT1具有作为非小细胞肺癌诊断标志物的潜能及肺癌早期辅助诊断价值。

Abstract:

Objective To investigate the expression level and diagnostic value of lncRNA NEAT1 in the plasma of patients with non-small cell lung cancer (NSCLC). Methods  qRT-PCR was used to detect the expression level of NEAT1 in plasma samples of 258 patients with NSCLC, 115 patients with benign lung diseases and 70 healthy controls, and then the differential expression levels of NEAT1 were compared in the 3 groups. The correlation between the expression level of NEAT1 in plasma and the clinical characteristics of patients with NSCLC was analyzed. Receiver operating characteristic (ROC) curve analysis was used to analyze the diagnostic value of the NEAT1 level in plasma for NSCLC. Results  In the NSCLC patients, the plasma level of NEAT1 was significantly higher than that in the healthy controls (P<0.01). The expression level of NEAT1 in the plasma was correlated with the age and tumor size of NSCLC patients, and was highly expressed in the >60 year-old group and the group whith tumor size >3 cm, with statistically significant differences (P<0.05). ROC curve results showed that plasma NEAT1 level had value for diagnosis of NSCLC, early stage NSCLC (stageⅠ+stageⅡ) or patients whose tumor size was equal to or less than 3 cm, with the areas under the curve of 0.72, 0.73 and 0.67, respectively. The plasma level of NEAT1 had a higher sensitivity (93%) as well as a higher negative predictive value (94%) to distinguish NSCLC with the tumor size ≤3 cm. Conclusion NEAT1 is highly expressed in the plasma of NSCLC patients. NEAT1 could be used as a potential diagnostic marker for NSCLC, and shows early diagnostic value for lung cancer.

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更新日期/Last Update: 2019-03-05