[1]唐相龙,龙宗泓,段光友,等.LOC339524基因介导脂多糖诱导小鼠小胶质bv2细胞系炎症因子的表达[J].第三军医大学学报,2019,41(04):289-295.
 TANG Xianglong,LONG Zonghong,DUAN Guangyou,et al.LOC339524 gene mediates lipopolysaccharide-induced expression of inflammatory cytokines in mouse microglia bv2 cell line[J].J Third Mil Med Univ,2019,41(04):289-295.
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LOC339524基因介导脂多糖诱导小鼠小胶质bv2细胞系炎症因子的表达(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第04期
页码:
289-295
栏目:
基础医学
出版日期:
2019-02-28

文章信息/Info

Title:
LOC339524 gene mediates lipopolysaccharide-induced expression of inflammatory cytokines in mouse microglia bv2 cell line
作者:
陆军军医大学(第三军医大学)第二附属医院麻醉科
Author(s):
TANG Xianglong LONG Zonghong DUAN Guangyou ZHOU Xiaoying CHEN Huifang LI Hong

Department of Anesthesiology, Second Affiliated Hospital, Army Military Medical University (Third Military Medical University), Chongqing, 400037, China

关键词:
脂多糖bv2细胞LOC339524TNF-&alphaIL-6
Keywords:
 
分类号:
R392-33; R392.11; R392.13
文献标志码:
A
摘要:

目的 研究LOC339524基因对脂多糖(lipopolysaccharide,LPS)诱导的小鼠小胶质bv2细胞系炎症因子表达的影响。方法 100 ng/mL LPS处理bv2细胞3、6、9、12 h用于摸索最佳炎症反应时间;10、100、1 000、10 000 ng/mL LPS处理bv2细胞和100 ng/mL LPS处理bv2细胞3、6、9、12 h用于研究LOC339524蛋白表达变化;siRNA沉默LOC339524和慢病毒转染构建过表达LOC339524的细胞株后加LPS处理用于检测炎症因子变化;qPCR检测TNF-α、IL-6和LOC339524 mRNA水平变化,Western blot检测TNF-α、IL-6和LOC339524蛋白水平变化。结果 与0 h相比,LPS处理不同时间均可使TNF-α、IL-6 mRNA和蛋白表达增加(P<0.05),12 h两者表达一致,选择12 h进行后续炎症反应研究;与0 ng/mL LPS处理组相比,不同浓度LPS均可诱导LOC339524蛋白的表达增加(P<0.05),100 ng/mL LPS处理后呈时间依赖性增加(P<0.05)。LPS处理12 h后,siRNA组较无关片段组,TNF-α和IL-6 mRNA和蛋白表达显著上调(P<0.05),反之,过表达组较正常组TNF-α和IL-6 mRNA和蛋白表达显著下调(P<0.05)。结论 LOC339524基因介导了脂多糖诱导bv2细胞的炎症反应。

Abstract:

Objective To study the role of LOC339524 gene in mediating the expression of inflammatory cytokines in mouse microglia bv2 cell line induced by lipopolysaccharide (LPS). Methods Mouse microglia bv2 cells were treated with 100 ng/mL LPS for 3, 6, 9 or 12 h to determine the optimal time for observing the inflammatory response. The cells were then treated with 10, 100, 1 000, and 10 000 ng/mL LPS or with 100 ng/mL LPS for 3, 6, 9, and 12 h, and the changes in the expression of LOC339524 protein was investigated. The changes in the expression of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in response to LPS challenge were detected in bv2 cells with siRNA-mediated LOC339524 gene silencing or lentivirusmediated LOC339524 overexpression using qPCR and Western blotting. Results Compared with blank control, LPS treatment increased TNF-α, IL-6 mRNA and protein expression at different times (P<0.05), and the expression was consistent at 12 hours. 12 hours were selected for subsequent inflammatory reaction studies;The expression of LOC339524 protein was increased viadifferent concentrations of LPS treatment(P<0.05) when compared with 0 ng/mL,and that increase was in a time-dependent manner at 100 ng/mL LPS concentration (P<0.05). After 12 hours of LPS treatment, the expression of TNF-α and IL-6 mRNA and protein was significantly upregulated in the siRNA group compared with the unrelated fragment group (P<0.05). Conversely the expression of TNF-α and IL-6 mRNA and protein in the over-expression group was significantly lower than that in the normal group (P<0.05).  Conclusion LOC339524 is an inflammatory response factor that promotes the inflammatory response of bv2 cells to LPS.

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更新日期/Last Update: 2019-02-21