[1]吴万江,袁继超,陈亚星,等.姜黄素抑制TNF-α诱导的人脐带间充质干细胞凋亡[J].第三军医大学学报,2019,41(04):282-288.
 WU Wanjiang,YUAN Jichao,CHEN Yaxing,et al.Curcumin inhibits tumor necrosis factor-α-induced apoptosis of human umbilical cord mesenchymal stem cells in vitro[J].J Third Mil Med Univ,2019,41(04):282-288.
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姜黄素抑制TNF-α诱导的人脐带间充质干细胞凋亡
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第04期
页码:
282-288
栏目:
基础医学
出版日期:
2019-02-28

文章信息/Info

Title:
Curcumin inhibits tumor necrosis factor-α-induced apoptosis of human umbilical cord mesenchymal stem cells in vitro
作者:
吴万江袁继超陈亚星倪飞陈欣王洁文泽贤张洪燕储卫华林江凯
陆军军医大学(第三军医大学)第一附属医院:神经外科,全军神经外科研究所,全军神经创伤防治重点实验室,全军眼科中心
Author(s):
 

Department of Neurosurgery, Institute of Neurosurgery, Key Laboratory of Neurotrauma Prevention and Treatment, Department of Ophthalmology, First Affiliated Hospital, Army Medical University  (Third Military Medical University), Chongqing, 400038, China

关键词:
姜黄素人脐带间充质干细胞TNF-&alpha细胞凋亡
Keywords:
curcumin human umbilical cord mesenchymal stem cells tumor necrosis factor-&alpha apoptosis 
分类号:
R285.5; R329-3; R329.28
文献标志码:
A
摘要:
目的 探讨姜黄素(curcumin,cur)对TNF-α诱导人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUC-MSC)凋亡的作用及机制。方法 体外培养人脐带间充质干细胞,给予5 ng/mL TNF-α诱导细胞凋亡,给予不同浓度cur干预,乳酸脱氢酶(LDH)释放实验检测细胞凋亡情况;选取4 μmol/L作为干预剂量,采用流式细胞仪检测细胞凋亡情况,进一步验证cur对TNF-α诱导的hUC-MSC凋亡的作用。Western blot检测肿瘤坏死因子-α受体(tumor necrosis factor receptor,TNFR)下游凋亡相关的MAPK家族信号通路 (ERK、JNK、p38)激活情况及Bcl-2、Bax的表达。结果 LDH释放实验结果提示:当TNF-α浓度高于5 ng/mL时导致hUC-MSC凋亡。cur浓度在0.5~4 μmol/L时可抑制TNF-α诱导的hUC-MSC凋亡,且呈浓度依赖性。流式细胞仪检测结果也证实4 μmol/L cur能显著抑制TNF-α诱导的hUC-MSC凋亡(P<0.05)。Western blot检测结果显示:cur可以激活ERK,促进Bcl-2的表达,使Bcl-2/Bax的表达升高(P<0.05),但不影响MAPK家族JNK、p38蛋白表达。结论 姜黄素能抑制TNF-α诱导的hUC-MSC凋亡,其作用机制可能是通过激活ERK,促进Bcl-2表达而实现。
 
Abstract:

Objective To investigate the inhibitory effect of curcumin on tumor necrosis factor-α (TNF-α)-induced apoptosis of human umbilical cord mesenchymal stem cells (hUC-MSCs) and explore the underlying mechanism. Methods hUC-MSCs cultured in vitro were treated with 5 ng/mL TNF-α and different concentrations of curcumin. LDH release assay and flow cytometry were used to assess the inhibitory effect of curcumin against TNF-α-induced cell apoptosis. Western blotting was used to detect the expression of the apoptosis-related molecules in the downstream MAPK signaling pathway (ERK, JNK, and p38) of tumor necrosis factor receptor (TNFR) and the apoptosis-related proteins (Bcl-2 and Bax). Results LDH release assay showed that TNF-α at a concentration above 5 ng/mL induced obvious apoptosis in cultured hUC-MSCs. Curcumin at the concentrations between 0.5 and 4 μmol/L significantly inhibited TNF-α-induced hUC-MSC apoptosis in a concentration-dependent manner; Flow cytometry also confirmed that 4 μmol/L curcumin significantly inhibited TNF-α-induced apoptosis of hUC-MSCs (P<0.05). The results of Western blotting showed that curcumin treatment caused ERK activation and enhanced the expression of Bcl-2 to increase the Bcl-2/Bax ratio (P<0.05) without obviously affecting the expression of JNK and p38 proteins in the cells. Conclusion Curcumin can inhibit TNF-α-induced apoptosis of hUC-MSCs possibly by activating ERK signaling pathway and enhancing Bcl-2 expression.

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更新日期/Last Update: 2019-02-21