[1]毛旭,马艳梅,孙妍娜.复合同种异体类雪旺细胞的异种神经支架修复面神经损伤的早期实验研究[J].第三军医大学学报,2019,41(02):116-123.
 MAO Xu,MA Yanmei,SUN Yanna.Repair of facial nerve defects in rabbits using xenogeneic nerve scaffolds seeded with composite Schwann-like cells[J].J Third Mil Med Univ,2019,41(02):116-123.
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复合同种异体类雪旺细胞的异种神经支架修复面神经损伤的早期实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第02期
页码:
116-123
栏目:
基础医学
出版日期:
2019-01-30

文章信息/Info

Title:
Repair of facial nerve defects in rabbits using xenogeneic nerve scaffolds seeded with composite Schwann-like cells
作者:
毛旭马艳梅孙妍娜
锦州医科大学附属第一医院:护理部,烧伤整形外科
Author(s):
MAO Xu MA Yanmei SUN Yanna

Department of Nursing, Department of Burns and Plastic Surgery, First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning Province, 121000, China

关键词:
面神经缺损脂肪间充质干细胞类雪旺细胞异种神经支架
Keywords:
facial nerve defects adipose tissue-derived mesenchymal stem cells Schwann-like cells xenogeneic nerve scaffold  
分类号:
R322.85; R329.2; R339.37
文献标志码:
A
摘要:

目的 探讨化学联合冻融方式处理的脱细胞异种神经支架复合体外培养的同种异体类雪旺细胞后修复面神经损伤的实验效果。方法取日本大耳白兔腹股沟脂肪垫,通过单酶消化法体外培养脂肪间充质干细胞(adiopose-deribed stem cell, ADSCs),并通过化学试剂及自体富血小板血浆(platelet rich plasma, PRP)体外定向诱导成类雪旺细胞(schwann cell-like cell lineages, SCs-like);取Wistar大鼠双侧坐骨神经,通过化学联合冻融的方式制备脱细胞异种神经支架。使用DiOC16(3)荧光探针进行类雪旺细胞的标记。建立兔面神经5 mm的缺损模型,以不同移植物确立3组实验分组(n=10):A组(自体神经修复组);B组(复合DiOC16-SCs的异种神经支架修复组);C组(单纯异种神经支架修复组)。术后8周通过再生神经的电生理、有髓神经纤维密度及特异性蛋白定量评价神经功能的早期修复效果。结果 术后各组动物分笼饲养,均存活良好。解剖时发现各组动物再生神经连续性及完整性均良好,未形成明显的神经瘤。术后8周各组术侧动作电位潜伏期延长,A、B组明显快于C组,且A组与B、C组比较差异均有统计学意义(P<0.05)。甲苯胺蓝染色计算再生有髓神经纤维密度,A、B组明显高于C组(P<0.05),A、B组之间比较差异无统计学意义。再生神经内特异性蛋白神经生长因子(nerve growth factor, NGF)及脑源性神经生长因子(brain-derived neurotrophic factor, BDNF)染色观察,可见A、B组内NGF及BDNF的蛋白含量均高于C组(P<0.05),且A组NGF蛋白含量最高,B组BDNF蛋白含量最高。结论 复合体外定向诱导的类雪旺细胞的脱细胞异种神经支架在修复面神经5 mm的缺损中早期获得的修复效果与自体神经的修复效果相近。

Abstract:

Objective To assess the effects of acellular xenogeneic nerve scaffold seeded with composite Schwann-like cells for repair of facial nerve defects in rabbits. Methods Adipose tissue-derived mesenchymal stem cells (ADSCs) were isolated from the groin fat pads of Japanese white rabbits using single enzyme digestion and were induced into Schwann cell-like cell lineages using chemical reagents and autologous plateletrich plasma. The acellular xenogeneic nerve scaffold was prepared by chemical treatment combined with freezing and thawing of the bilateral sciatic nerve grafts from Wistar rats. The Schwann-like cells were labeled with DiOC16(3) fluorescent probe before seeding onto the scaffold. In rabbit models of facial nerve defect (5 mm), the defect was repaired using autologous nerve grafts (group A, n=10), xenogeneic nerve scaffold seeded with the labeled Schwann-like cells (group B, n=10), or xenogeneic nerve scaffold only (group C, n=10). At 8 weeks after the operation, the nerve function was evaluated quantitatively by assessing the electrophysiology of the regenerated nerve, density of myelinated nerve fibers and expression of specific proteins. Results All the rabbit models survived after the operation. In all the rabbits, the regenerated nerve showed a good continuity and integrity without the formation of obvious neuromas. At 8 weeks after the operation, the latency of the action potential of the repaired facial nerve was prolonged in all the groups; the latency was significantly shorter in groups A and B than in group C, and was the shortest in group A (P<0.05). The density of the regenerated myelinated nerve fibers, as calculated using toluidine blue staining, was significantly higher in groups A and B than in group C (P<0.05) and similar between groups A and B. The expression levels of nerve growth factor (NGF) and brainderived neurotrophic factor (BDNF) in the regenerated nerve were significantly higher in groups A and B than in group C (P<0.05); the expression of NGF was the highest in group A, and BDNF expression was the highest in group B. ConclusionThe acellular xenogeneic scaffold seeded with Schwann-like cells achieves similar effect to autogenous nerve graft for repairing facial nerve defect in the early stage of injury.

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更新日期/Last Update: 2019-01-22