[1]吴宏烨,李锴,王芬,等.原头蚴体外形成棘球蚴的优化培养体系研究[J].第三军医大学学报,2019,41(05):415-423.
 WU Hongye,LI Kai,WANG Fen,et al.Culture systems for transformations of hydatid cysts from protoscoleces in vitro[J].J Third Mil Med Univ,2019,41(05):415-423.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第05期
页码:
415-423
栏目:
基础医学
出版日期:
2019-03-15

文章信息/Info

Title:
Culture systems for transformations of hydatid cysts from protoscoleces in vitro
作者:
吴宏烨李锴王芬刘许诺范俊杰叶彬
重庆医科大学基础医学院病原生物学教研室,分子医学与肿瘤研究中心
Author(s):
WU Hongye LI Kai WANG Fen LIU Xunuo FAN Junjie YE Bin

Research Center for Molecule Medicine and Tumor, Department of Pathogenic Biology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China

关键词:
细粒棘球绦虫原头蚴成囊棘球蚴培养体系
Keywords:
Echinococcus granulosus protoscoleces encystation hydatid culture system  
分类号:
Q95-331; Q959.175; R383.33
文献标志码:
A
摘要:

目的 建立适合细粒棘球绦虫原头蚴在体外形成棘球蚴的培养体系。 方法 以RPMI1640或DMEM培养基为基质,胎牛血清或绵羊血清为营养成分,人肝细胞株L02为饲养细胞设计配伍2大类、6个组培养体系;培养细粒棘球绦虫原头蚴45 d,根据各组原头蚴的头节外翻率、成囊率和囊泡大小情况,寻找适合原头蚴形成棘球蚴的体外培养体系最佳配伍;在最佳配伍的培养体系继续培养棘球蚴囊泡至180 d。 结果 在起初的45 d期间,DMEM体系的E组(DMEM+胎牛血清+人肝细胞株L02)培养的棘球蚴生长最快,20 d起原头蚴成囊率高于其他各组(P<0.05),25 d起棘球蚴囊泡大于其他各组(P<0.05),45 d时原头蚴成囊率达到100%;在其后的46~180 d内,棘球蚴囊泡持续增大,至80 d时肉眼可见透明囊泡,至180 d时最大囊泡直径达2.2 mm。 结论 添加胎牛血清及人肝细胞株L02的DMEM培养基有利于体外培养的原头蚴发育为棘球蚴囊泡。

Abstract:

Objective To search for a suitable culture system for transforming protoscoleces of Echinococcus granulosus into hydatid cysts in vitro. Methods Based on the basic cell culture media RPMI1640 or DMEM, and fetal calf serum or sheep serum as nutrients, and human liver cell line L02 as server cells, culture systems for transformations of protoscoleces into hydatid cysts in vitro were established based on block designs. The culture systems were divided into 2 main groups, and 6 subgroups according the different ingredients of culture media, sera, and sever cells. The protoscoleces were cultured in 6 subgroups for 45 d, and then the most suitable medium for the transformations was screened according to the ratios of evaginated or encysted protoscoleces, and the sizes of protoscoleces. In the selected best culture system, the encysted protoscoleces were kept cultivating up to 180 d for further observation. Results Within the first period of 45 d, it demonstrated that the culture system of DMEM medium+fetal calf serum+L02 cells was the best culture system for transformations of protoscoleces into hydatid cysts in vitro, because of the fastest growth speed of protoscoleces, the maximal ratio of encystation of protoscoleces since 20 d (P<0.05), the biggest size of hydatid cysts since 25 d (P<0.05), and 100% percentage of encystation of protoscoleces at 45 d. In the following culture days (46 to 180 d), the hydatid cysts still grew up, the transparent hydatid cysts were visible to the naked eyes at 80 d, and the biggest cyst size reached to 2.2 mm at 180 d. Conclusion The culture medium of DMEM, added with fetal calf serum and L02 cells, is a suitable culture system for transformations of protoscoleces into hydatid cysts in vitro.

 

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更新日期/Last Update: 2019-03-05