[1]杨佳丹,向荣凤,戴青,等.人参皂苷Rg3对模拟高原缺氧大鼠的抗疲劳效应和骨骼肌线粒体功能的影响[J].第三军医大学学报,2019,41(02):110-115.
　YANG Jiadan,XIANG Rongfeng,DAI Qing,et al.Effects of ginsenoside Rg3 on fatigue resistance and skeletal muscle mitochondrial function in rats exposed to a simulated altitude of 5 000 m[J].J Third Mil Med Univ,2019,41(02):110-115.
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人参皂苷Rg3对模拟高原缺氧大鼠的抗疲劳效应和骨骼肌线粒体功能的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
41卷
期数:
2019年第02期
页码:
110-115
栏目:
基础医学
出版日期:
2019-01-30

文章信息/Info

Title:
Effects of ginsenoside Rg3 on fatigue resistance and skeletal muscle mitochondrial function in rats exposed to a simulated altitude of 5 000 m
作者:
杨佳丹;  向荣凤; 戴青; 来小丹
重庆医科大学附属第一医院药学部；陆军军医大学(第三军医大学)第一附属医院药剂科
Author(s):
YANG Jiadan;  XIANG Rongfeng;  DAI Qing;  LAI Xiaodan

Department of Pharmacy, First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016;  Department of Pharmacy, First Affiliated Hospital,  Army Medical University (Third Military Medical University), Chongqing, 400038, China

关键词:
人参皂苷Rg3; 抗疲劳; 高原; 缺氧; 骨骼肌线粒体
Keywords:
ginsenoside Rg3;  fatigue resistance;  high altitude;  hypoxia;  skeletal muscle mitochondria
分类号:
R285.5; R322.74; R339.54
文献标志码:
A
摘要:

目的 观察人参皂苷Rg3在模拟高原环境下提高大鼠的抗疲劳能力和对骨骼肌线粒体功能的影响。方法40只大鼠按随机数字表法分成4组，每组10只，分为正常对照组、Rg3+正常对照组（NG+Rg3）、缺氧模型组（model group, MG）、Rg3+缺氧模型组(MG+Rg3)。MG组和MG+Rg3组在模拟海拔 5 000 m高度的低压舱中喂养，大鼠每日从模拟高原环境中取出1 h行Rg3灌胃，剂量为20 mg/(kg·d)。连续灌胃15 d后，观察大鼠在正常环境和模拟高原环境下力竭游泳时间。力竭游泳实验结束后30 min，腹主动脉采血并检测大鼠总胆固醇(total cholesterol，TC)、甘油三酯(triglyceride，TG)、乳酸脱氢酶(lactate dehydrogenase，LDH)、血糖(glucose, GLU)、血氨（blood ammonia，BA）和血尿素氮（blood urea nitrogen，BUN）的变化。同时迅速分离大鼠股四头肌，检测骨骼肌线粒体丙二醛（malondialdehyde, MDA)、锰超氧化物歧化酶(Mn-superoxide dismutase, Mn-SOD)以及呼吸链复合物Ⅲ、Ⅳ活性的变化。结果 Rg3显著延长正常环境和模拟高原环境下大鼠的力竭游泳时间（P<0.01）；显著上调正常环境和模拟高原环境下大鼠血清TC、TG、LDH、GLU浓度（P<0.05，P<0.01）和骨骼肌线粒体Mn-SOD活性及骨骼肌线粒体呼吸链复合物Ⅲ、Ⅳ活性（P<0.01）；显著下调模拟高原环境下大鼠的BUN含量和骨骼肌线粒体MDA含量（P<0.01）。结论 人参皂苷Rg3在模拟高原环境下可延长大鼠力竭游泳时间，改善疲劳相关的生化指标，提高骨骼肌线粒体对自由基的消除作用和供能效力。

Abstract:

Objective   To observe the effects of ginsenoside Rg3 (Rg3) in improving fatigue resistance and functionality of skeletal muscle mitochondria in rats at a simulated high altitude. Methods   Forty adult male SD rats were randomly divided into 4 groups (n=10), namely the normal group (NG), Rg3-treated normal group (NG+Rg3), model group (MG), and Rg3-treated model group (MG+Rg3). In the latter 2 groups, the rats were exposed to a stimulated altitude of 5 000 m in a hypobaric chamber, and on a daily basis, the rats were taken from the chamber for intragastric administration of 20 mg/kg Rg3 (completed in 1 h). After 15 d of treatment, forced swimming test was performed and 30 min later blood samples were collected from the abdominal aorta for testing the levels of total cholesterol (TC), plasma triglyceride (TG), lactate dehydrogenase (LDH), blood glucose (GLU), blood ammonia (BA) and blood urea nitrogen (BUN); The quadriceps were quickly isolated for detecting malondialdehyde (MDA), Mn-superoxide dismutase (Mn-SOD) and the activity of compound Ⅲ and Ⅳ in the respiratory chain. Results    Rg3 treatment significantly increased the swimming time (P<0.05 or 0.01) and serum levels of TC, TG, LDH and GLU (P<0.05 or 0.01) and enhanced Mn-SOD activity and mitochondrial respiratory chain complex Ⅲ and Ⅳ activities  (P<0.01) both in rats kept in normal conditions and in those exposed to the simulated high altitude. Rg3 also significantly decreased serum BUN level and MDA content in the skeletal muscle mitochondria in rats exposed to the simulated high altitude (P<0.01). Conclusion   Rg3 can extend forced swimming time, improve the biochemical indexes related to fatigue, and enhance free radical clearance and energy supply of skeletal muscle mitochondria of rats

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更新日期/Last Update: 2019-01-22