[1]王力伟,周晓洲,陈志文,等.膀胱癌循环肿瘤细胞NANOG基因的表达与临床特征相关性的初步研究[J].陆军军医大学学报(原第三军医大学学报),2018,40(08):711-716.
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膀胱癌循环肿瘤细胞NANOG基因的表达与临床特征相关性的初步研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第08期
页码:
711-716
栏目:
临床医学
出版日期:
2018-04-30

文章信息/Info

Title:
Correlation of Nanog expression in circulating tumor cells with clinical characteristics of patients with bladder cancer
作者:
王力伟周晓洲陈志文杨劲
陆军军医大学(第三军医大学):第一附属医院全军泌尿外科研究所1,基础医学院细胞生物教研室
Author(s):
WANG Liwei ZHOU Xiaozhou CHEN Zhiwen YANG Jin

Institute of Urology, First Affiliated Hospital, 2Department of Cell Biology, College of Basic Medical Sciences, Army Medical University (Third Military Medical University), Chongqing, 400038, China

关键词:
膀胱癌循环肿瘤细胞Nanog上皮间质转化转移
Keywords:
bladder cancer circulating tumor cells Nanog epithelial-mesenchymal transition metastasis
分类号:
R181.32;R730.43;R737.14
文献标志码:
A
摘要:

目的    探讨膀胱癌患者外周血循环肿瘤细胞(circulating tumor cells,CTCs)及其中Nanog基因的阳性表达率与临床特征的相关性。  方法    选取2015年12月至2016年8月陆军军医大学第一附属医院全军泌尿外科研究所收治的膀胱尿路上皮癌患者59例,采用广州益善生物技术股份有限公司开发的CanpatrolTM技术检测患者外周血中的CTCs分布情况及CTCs中Nanog基因的表达水平,分析上述检测结果与患者性别、年龄、肿瘤分级、浸润程度、淋巴结活检以及转移情况之间的相关性。结果    肌层浸润性膀胱癌患者外周血中CTCs阳性率高于非肌层浸润性膀胱癌患者(33/36,91.7% vs 14/23,60.9%,P=0.007);淋巴结活检阳性膀胱癌患者CTCs阳性率高于淋巴结活检阴性患者(19/20,95.0% vs 28/39,71.8%,P=0.044);混合型CTCs中Nanog基因的阳性率高于上皮型(22/56,39.3% vs 96/176,54.5%,P=0.047)。结论    CTCs可作为判断膀胱癌恶性程度和侵袭能力的辅助指标,CTCs中的Nanog基因有望成为评估膀胱癌转移和预后的生物标志。

Abstract:

Objective    To investigate the correlation between Nanog expression in circulating tumor cells (CTCs) and the clinical features of patients with bladder cancer.  Methods    CTCs were isolated from 59 patients with bladder urothelial carcinoma and classified into epithelial, mesenchymal and bi-phenotypic CTCs. Nanog expression in the CTCs was detected using CanpatrolTM technique. The positivity of CTCs and Nanog positivity in the CTCs were analyzed in these patients in relation with the patients' clinical features.  Results    The positivity rate of CTCs was significantly higher in patients with muscle invasive bladder cancer (MIBC) than in those with non-muscle invasive bladder cancer (NMIBC) [41/44 (93.2%) vs 6/15 (40.0%), P=0.007], and was also significantly higher in the patients with positive biopsy findings of the lymph nodes than in those with negative findings [19/20 (95.0%) vs 28/39 (71.8%), P=0.044]. Bi-phenotypic CTCs were found to have a higher rate of Nanog positivity than epithelial CTCs [22/56 (39.3%) vs 96/176 (54.5%), P=0.047].  Conclusion    CTCs positivity can assist in the evaluation of malignancy and invasiveness of bladder cancer, and Nanog expression in the CTCs has the potential to serve as a biomarker for evaluating metastasis and prognosis of bladder cancer.
 

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更新日期/Last Update: 2018-04-28