[1]肖磊,田跃,柯志刚,等.慢传输性便秘患者结肠黏膜DNA甲基转移酶表达显著增高[J].第三军医大学学报,2018,40(08):705-710.
 XIAO Lei,TIAN Yue,KE Zhigang,et al.Expression of DNA methyltransferase is significantly increased in the colonic mucosa of patients with slow transit constipation[J].J Third Mil Med Univ,2018,40(08):705-710.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第08期
页码:
705-710
栏目:
基础医学
出版日期:
2018-04-30

文章信息/Info

Title:
Expression of DNA methyltransferase is significantly increased in the colonic mucosa of patients with slow transit constipation
作者:
肖磊田跃柯志刚张勇童卫东
陆军军医大学(第三军医大学)第三附属医院野战外科研究所普通外科
Author(s):
XIAO Lei TIAN Yue KE Zhigang ZHANG Yong TONG Weidong

Department of General Surgery, Center of Constipation Diagnosis and Treatment, Institute of Surgery Research, Third Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China

关键词:
慢传输性便秘结肠黏膜DNA甲基转移酶
Keywords:
slow transit constipation colonic mucosa DNA methyltransferase
分类号:
R322.45;R345.5;R574.62
文献标志码:
A
摘要:

目的    研究DNA甲基转移酶(DNA methyltransferase, Dnmt)在慢传输性便秘(slow transit constipation, STC)结肠黏膜中的表达。方法    收集2015年9月至2017年6月我科收治的8例STC患者乙状结肠标本,采用免疫组织化学染色、Western blot检测Dnmt1、Dnmt3a、Dnmt3b蛋白在结肠黏膜的表达情况,real-time qRTPCR检测结肠黏膜Dnmt1、Dnmt3a、Dnmt3b mRNA相对表达量。对照组标本采用直肠癌患者术中切除的远离癌灶的近端乙状结肠。结果     免疫组织化学染色结果显示:对照组Dnmt1、Dnmt3a、Dnmt3b IOD值分别为(705±362)、(538±206)、(359±238),STC组Dnmt1、Dnmt3a、Dnmt3b IOD值分别为(4 077±1 219)、(4 214±1 858)、(3 026±1 619),两组比较差异有统计学意义(P<0.05);Western blot检测结果显示:对照组Dnmt1、Dnmt3a、Dnmt3b蛋白相对表达量分别为(0.663±0.349)、(0.250±0.159)、(0.330±0.195),STC组Dnmt1、Dnmt3a、Dnmt3b蛋白相对表达量分别为(1.475±0.372)、(1.035±0.362)、(0.977±0.258),两组比较差异有统计学意义(P<0.05);Real-time qRT-PCR结果显示:对照组Dnmt1、Dnmt3a、Dnmt3b mRNA相对表达量分别为(0.285±0.121)、(0.069±0.021)、(0.512±0.112),STC组Dnmt1、Dnmt3a、Dnmt3b mRNA相对表达量分别为(0.563±0.172)、(0.340±0.083)、(0.964±0.219),两组比较差异有统计学意义(P<0.05)。结论    STC患者结肠黏膜Dnmt1、Dnmt3a、Dnmt3b表达显著增高,可能是STC发病的重要环节。
 

Abstract:

Objective    To investigate the expression of DNA methyltransferase (Dnmt) in the colonic mucosa of patients with slow transit constipation (STC).  Methods    Surgical specimens of the sigmoid colon were collected from 8 patients with STC and 8 patients with rectal cancer admitted in our hospital between September, 2015 and June, 2017. Only the adjacent colon tissues (at least 10 cm from the tumor margin) from the rectal cancer patients were used to serve as the control. The expressions of Dnmt1, Dnmt3a and Dnmt3b proteins in the colonic mucosa were detected using immunochemistry and Western blotting, and their mRNA expressions were quantified with real-time qRT-PCR. Results    The results of immunochemical staining showed that the integral optical densities (IOD) of Dnmt1, Dnmt3a and Dnmt3b expressions in the control group were 705±362, 538±206, and 359±238, respectively, significantly lower than those in STC group (4 077±1 219, 4 214±1 858, and 3 026±1 619, respectively; P<0.05). Western blotting also showed significantly lower relative expressions of Dnmt1, Dnmt3a and Dnmt3b proteins in the control group than in STC group (0.663±0.349, 0.250±0.159, and 0.330±0.195 vs 1.475±0.372, 1.035±0.362, and 0.977±0.258, respectively; P<0.05). Real-time qRT-PCR yielded consistent results of the relative expressions of Dnmt1, Dnmt3a and Dnmt3b mRNAs in the control and STC groups (0.285±0.121, 0.069±0.021, and 0.512±0.112 vs 0.563±0.172, 0.340±0.083, and 0.964±0.219, respectively; P<0.05).  Conclusion    High expressions of Dnmt1, Dnmt3a and Dnmt3b in the colonic mucosa may be an important factor in the pathogenesis of STC.

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更新日期/Last Update: 2018-04-28