[1]高振,吴琴,郭俊峰,等.降钙素基因相关肽对人脐带间充质干细胞成骨分化的影响[J].第三军医大学学报,2018,40(06):479-486.
 GAO Zhen,WU Qin,GUO Junfeng,et al.Effects of calcitonin gene-related peptide on osteogenic differentiation of human umbilical cord mesenchymal stem cells[J].J Third Mil Med Univ,2018,40(06):479-486.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
40卷
期数:
2018年第06期
页码:
479-486
栏目:
基础医学
出版日期:
2018-03-30

文章信息/Info

Title:
Effects of calcitonin gene-related peptide on osteogenic differentiation of human umbilical cord mesenchymal stem cells
作者:
高振吴琴郭俊峰李铀陈雪丹高强国张纲
陆军军医大学(第三军医大学)第二附属医院口腔科;陆军军医大学(第三军医大学)基础医学院医学遗传学教研室
Author(s):
GAO Zhen WU Qin GUO Junfeng LI You CHEN Xuedan GAO Qiangguo ZHANG Gang

Department of Stomatology, Second Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing,400037; 2Department of Medical Genetics, College of Basic Medical Sciences, Army Medical University (Third Military Medical University), Chongqing, 400038,China

关键词:
降钙素基因相关肽人脐带间充质干细胞成骨细胞细胞分化
Keywords:
calcitonin gene-related peptide human umbilical cord mesenchymal stem cells osteoblasts cell differentiation
分类号:
R322.71;R329.21;R394.3
文献标志码:
A
摘要:

目的    探讨降钙素基因相关肽(calcitonin gene related peptide,CGRP)在人脐带间充质干细胞(human umbilical cord mesenchymalstem cells,HUMSCs)向成骨细胞分化过程中的作用及机制。方法    体外培养HUMSCs细胞,加入0、10-9、10-8、10-7 mol/L浓度的CGRP诱导其向成骨细胞分化,分别在第7、9、11、14天时采用茜素红染色检测成骨分化程度,实时定量PCR和Western blot检测ERK、RUNX2、OSTERIX的mRNA和磷酸化的ERK、RUNX2、OSTERIX的蛋白表达。结果    HUMSCs高表达CD73、CD90、CD105等间充质干细胞表面抗原,但不表达CD14、CD20、CD34、CD45等上皮和造血干细胞的表面抗原,可诱导向成骨细胞分化,随着时间延长矿化结节数量增加(P<0.05);随着CGRP浓度的升高,矿化结节减少,ERK、RUNX2、OSTERIX的mRNA和蛋白表达降低(P<0.05)。结论    CGRP通过ERK信号通路抑制HUMSCs向成骨细胞的分化,并呈现出浓度和时间依赖性。

Abstract:

Objective    To investigate the role and underlying mechanism of calcitonin gene-related peptide (CGRP) in the differentiation of human umbilical cord mesenchymal stem cells (HUMSCs) into osteoblasts. Methods    Different concentrations (0,10-9,10-8 and 10-7 mol/L) of CGRP were added into the culture media of HUMSCs to induce differentiation into osteoblasts. In 7,9,11 and 14 d after treatment, Alizarin red staining was used to detect the differentiation of osteogenesis. Real-time PCR and Western blotting were employed to detect the mRNA expression levels of ERK, RUNX2 and OSTERIX and protein expression levels of phosphorylation of ERK, RUNX2 and OSTERIX. Results    HUMSCs with high expression of CD73, CD90, CD105 and other mesenchymal stem cell surface antigens, but not with the expression of CD14, CD20, CD34, CD45 and other epithelial and hematopoietic stem cell surface antigens, were identified to induce differentiation into osteoblasts. The number of mineralized nodules was increased with time elapsing (P<0.05), while the number was decreased with the increasing concentrations of CGRP, and the mRNA and protein expression levels of ERK, RUNX2 and OSTERIX were decreased (P<0.05). Conclusion    CGRP inhibits the differentiation of HUMSCs into osteoblasts by ERK signaling pathway in a concentration- and timedependent manner.

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更新日期/Last Update: 2018-03-23