[1]王五艺,程勇,万林.AGEs通过JAK2/STAT3信号通路诱导结肠癌SW480细胞增殖、侵袭及上皮间质转化[J].第三军医大学学报,2017,39(24):2390-2395.
 WANG Wuyi,CHENG Yong,WAN Lin.Advanced glycation end products enhance proliferation and invasion of human colon cancer SW480 cells through JAK2/STAT3 pathway[J].J Third Mil Med Univ,2017,39(24):2390-2395.
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AGEs通过JAK2/STAT3信号通路诱导结肠癌SW480细胞增殖、侵袭及上皮间质转化(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第24期
页码:
2390-2395
栏目:
基础医学
出版日期:
2017-12-30

文章信息/Info

Title:
Advanced glycation end products enhance proliferation and invasion of human colon cancer SW480 cells through JAK2/STAT3 pathway
作者:
王五艺程勇万林
重庆医科大学附属第一医院胃肠外科
Author(s):
WANG WuyiCHENG Yong WAN Lin

Department of Gastrointestinal Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

关键词:
结直肠肿瘤糖基化终末产物增殖上皮间质转化JAK2/STAT3
Keywords:
colorectal neoplasms advanced glycation products proliferation epithelial-mesenchymaltransition JAK2/STAT3
分类号:
R394.2;R730.23;R735.35
文献标志码:
A
摘要:

目的     观察糖基化终末产物(advanced glycation end products, AGEs)对结肠癌SW480细胞增殖、凋亡、侵袭、迁移及上皮间质转化的影响,并探讨其相关机制。方法     将结肠癌SW480细胞分为对照组、AGEs组(200 μg/mL AGEs)、AGEs(200 μg/mL)+AG490(50 μmol/L)组,CCK-8检测细胞增殖活力,流式细胞术检测细胞周期、凋亡,Transwell实验检测细胞体外侵袭能力,划痕实验检测细胞体外迁移能力。不同浓度(0、50、100、200 μg/mL)AGEs和200 μg/mL AGEs、50 μmol/L AG490单独处理及二者联合处理SW480细胞后,Western blot检测上皮间质转化相关分子标志物E-cadherin、N-cadherin、Vimentin及JAK2/STAT3信号通路相关分子的变化。结果    与对照组比较,AGEs能明显促进结肠癌SW480细胞的增殖,减少G1/S期阻滞,抑制凋亡,增强体外的侵袭、迁移能力(P<0.05)。与AGEs组比较,AGEs+AG490组结肠癌SW480细胞增殖减少,G1/S期阻滞增加,凋亡增加,侵袭、迁移能力降低,差异有统计学意义(P<005)。Western blot检测结果显示,AGEs处理结肠癌SW480细胞后E-cadherin表达降低,N-cadherin、Vimentin、p-STAT3、p-JAK2表达升高,而AG490可逆转AGEs对结肠癌SW480细胞的作用。结论     AGEs能够通过JAK2/STAT3信号通路促进结肠癌SW480细胞的增殖,抑制凋亡,增强侵袭、迁移能力,促进上皮间质转化。

Abstract:

Objective       To explore the effects and the possible mechanisms of advanced glycation products (AGEs) on the proliferation,invasion and epithelial-mesenchymal transition(EMT) of human colon cancer cell line SW480. Methods      The SW480 cells were divided into control group, AGEs (200 μg/mL) group, and AGEs (200 μg/mL)+AG490(50 μmol/L) group. After the treatment, the cell proliferation, cell cycle and apoptosis, invasion and migration were tested by CCK-8 assay, flow cytometry, Transwell chamber test and wound healing assay. After the cells were treated with 0, 50, 100 and 200 μg/mL AGEs or 200 μg/mL AGEs plus 50 μmol/L AG490, the expression levels of EMT markers, E-cadherin, N-cadherin and Vimentin, and JAK2/STAT3 pathway molecules, STAT3, p-STAT3, JAK2 and p-JAK2 were detected by Western blotting. Results     Compared with the control group, AGEs significantly promoted the proliferation,invasion and migration of SW480 cells, reduced the number of cells arrested at G1/S phase, and inhibited cell apoptosis (P<0.05). Whereas, these effects were obviously reversed by the addition of 50 μmol/L AG490  (P<0.05). Western blotting showed that AGEs increased the expression levels of N-cadherin, Vimentin, p-STAT3 and p-JAK2, and decreased that of E-cadherin. But AG490 reversed such changes resulted from AGEs treatment. Conclusion      AGEs enhance proliferation, invasion and migration, and promote EMT in human colon cancer cell line SW480 through JAK2/STAT3 signaling pathway.

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更新日期/Last Update: 2017-12-26