[1]邵艳霞,孙航,周小萍,等.急性创伤应激对小鼠行为变化的影响[J].第三军医大学学报,2017,39(11):1130-1136.
 Shao Yanxia,Sun Hang,Zhou Xiaoping,et al.Effect of acute traumatic stress on behavioral changes in mice[J].J Third Mil Med Univ,2017,39(11):1130-1136.
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急性创伤应激对小鼠行为变化的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第11期
页码:
1130-1136
栏目:
基础医学
出版日期:
2017-06-15

文章信息/Info

Title:
Effect of acute traumatic stress on behavioral changes in mice
作者:
邵艳霞孙航周小萍孙溦
第三军医大学西南医院急救部
Author(s):
Shao Yanxia Sun Hang Zhou Xiaoping Sun Wei

Department of Emergency, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China

关键词:
急性应激行为变化小鼠
Keywords:
acute stress behavior change mice
分类号:
R-332; R338.64; R64
文献标志码:
A
摘要:

目的       研究急性创伤应激对小鼠自发行为、焦虑、学习记忆的影响。方法       将16只小鼠分为实验组(n=8)和对照组(n=8),实验组给予足底电击,对照组不给予任何处理;分别在处理后0 h、12 h、7 d进行旷场实验以及高架十字迷宫实验,以评价小鼠行为学改变;用水迷宫实验以评价小鼠学习记忆改变。结果      (1)两组小鼠的站立次数和中央区域活动时间、路程在应激后0 h和12 h差异均有统计学意义(P<0.01),实验组少于对照组;应激后7 d两组仅中央活动时间差异有统计学意义(P<0.05);(2)实验组小鼠进入开臂次数及停留时间在应激后立即、12 h较对照组均显著减少(P<0.05);(3)实验组小鼠第2、3天的逃避潜伏期较对照组显著延长(P<0.05),穿越平台次数及目标象限游程比例较对照组均显著增多(P<0.05)。结论      足底电击可引起小鼠自发行为减少,导致探索样行为减弱,焦虑增加,学习能力降低,但在一定程度上增强小鼠的空间记忆能力。

Abstract:

Objective       To determine the effect of acute traumatic stress on spontaneous behaviors, anxiety, learning and memory in mice. Methods       Sixteen mice were randomly divided into experimental group (n=8) and control group (n=8). The mice of experimental group were given plantar shock while those of the control group were not. At the initial time after plantar shock (0 h), and in 12 h and 7 d after the infliction, open field test and elevated plus maze test were used to observe the behavior changes, and water maze test was employed for the changes of learning and memory. Results       ①The standing time and the central activity time and distance showed significant differences between the 2 groups in 0 and 12 h after stress (P<0.01), with the experimental group less than control group, but significant difference was observed in the central activity time on the 7th day (P<0.05).   ②The number of enter open arms and residence time in open arms were significantly reduced in the mice from the experimental group than the control group at immediate and 12 h after stress (P<0.05).   ③The escape latency was significantly prolonged in the experimental group than the control group on the 2nd and 3rd days (P<0.05), and the crossing platform times and target quadrant run ratio were also obviously larger than control group (P<0.05). Conclusion       Plantar electric shock can lead the decrease of spontaneous behavior in mice, decrease the exploratory behavior and learning ability, and increase the anxiety behavior, and to some extent, enhance spatial memory ability.

参考文献/References:

[1]Kassi E, Pervanidou P, Kaltsas G, et al. Metabolic syndrome: definitions and controversies[J]. BMC Med, 2011, 9: 48. DOI:10.1186/1741-7015-9-48.
[2]McEwen B S. Central effects of stress hormones in health and disease: Understanding the protective and damaging effects of stress and stress mediators[J]. Eur J Pharmacol, 2008, 583(2/3): 174-185. DOI:10.1016/j.ejphar.2007.11.071.
[3]杜建政, 夏冰丽. 急性应激障碍(ASD)研究述评[J]. 心理科学进展, 2009, 17(3): 482-488.
Du J Z, Xia B L. A review of study on acute stress disorder[J].Advances in Psychological Science,2009,17(3): 482-488..
[4]Wilson M A, Grillo C A, Fadel J R, et al. Stress as a one-armed bandit: Differential effects of stress paradigms on the morphology, neurochemistry and behavior in the rodent amygdala[J]. Neurobiol Stress, 2015, 1:195-208. DOI:10.1016/j.ynstr.2015.06.001.
[5]李欢欢, 林文娟, 李俊发. 急性生理应激对大鼠的行为及脑神经颗粒素磷酸化水平的影响[J]. 心理学报, 2007, 39(4): 648-655.
Li H H, Lin W J, Li J F. The effects of an acute physiological stressor on behaviors, protein levels and phosphorylation of neurogranin in rats[J]. Acta Psychologica Sinica, 2007, 39(4): 648-655.
[6]王倩, 郑晓星, 杨蕴萍. 急性应激障碍能否预测创伤后应激障碍[J]. 首都医科大学学报, 2008, 29(5): 564-568. DOI:10.3969/j.issn.1006-7795.2008.05.006.
Wang Q, Zheng X X, Yang Y P. Can acute stress disorder predict posttraumatic stress disorder[J]. Journal of Capital Medical University, 2008, 29(5): 564-568. DOI:10.3969/j.issn.10067795.2008.05.006.
[7]Kingsbury T J, Krueger B K. Ca2+, CREB and krüppel: a novel KLF7-binding element conserved in mouse and human TRKB promoters is required for CREB-dependent transcription[J]. Mol Cell Neurosci, 2007, 35(3): 447-455. DOI:10.1016/j.mcn.2007.04.004.
[8]Stam R.PTSD and stress sensitisation: A tale of brain and body.Part 2: Animal models[J]. Neurosci Biobehav Rev,2007,31(4):558-584.DOI:10.1016/j.neubiorev.2007.01.001.
[9]Ekdahl C T, Kokaia Z, Lindvall O. Brain inflammation and adult neurogenesis: the dual role of microglia[J]. Neuroscience, 2009,158(3):1021-1029.DOI:10.1016/j.neuroscience.2008.06.052.
[10]Deng W, Aimone J B, Gage F H. New neurons and new memories: how does adult hippocampal neurogenesis affect learning and memory?[J]. Nat Rev Neurosci, 2010, 11(5): 339-350. DOI:10.1038/nrn2822.
[11]Hawley W, Grissom E, Keskitalo L, et al. Sexual motivation and anxiety-like behaviors of male rats after exposure to a trauma followed by situational reminders[J]. Physiol Behav, 2011, 102(2): 181-187. DOI:10.1016/j.physbeh.2010.10.021.
[12]Novaes L S, Dos Santos N B, Batalhote R F, et al. Environmental enrichment protects against stressinduced anxiety: Role of glucocorticoid receptor, ERK, and CREB signaling in the basolateral amygdala[J]. Neuropharmacology, 2017, 113(Pt A): 457-466. DOI:10.1016/j.neuropharm. 2016.10.026.
[13]Ronzoni G, Del Arco A, Mora F, et al. Enhanced noradrenergic activity in the amygdala contributes to hyperarousal in an animal model of PTSD[J]. Psychoneuroendocrinology, 2016, 70: 1-9. DOI:10.1016/j.psyneuen.2016.04.018.
[14]Haghparast A, Fatahi Z, Alamdary S Z, et al. Changes in the levels of p-ERK, p-CREB, and cfos in rat mesocorticolimbic dopaminergic system after morphineinduced conditioned place preference: the role of acute and subchronic stress[J]. Cell Mol Neurobiol, 2014, 34(2): 277-288. DOI:10.1007/s10571-013-0011-z.
[15]VilaVerde C, Marinho A L, Lisboa S F, et al. Nitric oxide in the prelimbic medial prefrontal cortex is involved in the anxiogenic-like effect induced by acute restraint stress in rats[J]. Neuroscience, 2016, 320: 30-42. DOI:10.1016/j. neuroscience.2016.01.040.
[16]Clark R E, Broadbent N J, Squire L R. The hippocampus and spatial memory: findings with a novel modification of the water maze[J]. J Neurosci, 2007, 27(25): 6647-6654. DOI:10.1523/JNEUROSCI.0913-07.2007.
[17]Morris R. Developments of a watermaze procedure for studying spatial learning in the rat[J]. J Neurosci Methods, 1984, 11(1): 47-60. DOI:10.1016/0165-0270(84)90007-4.
[18]Wittner M, Ríha P. Transient hypobaric hypoxia improves spatial orientation in young rats[J]. Physiol Res, 2005, 54(3): 335-340.
[19]孙伊思. 急性应激期大鼠学习敏感化的改变及其对PTSD样症状形成的预测作用[D]. 长沙:湖南师范大学, 2012.DOI:    10.7666/d.y2148260.
Sun Y S.The changes in learning sensitive of acute stress in rats and predict the symptoms of PTSD[D].Chang Sha:Hunan Normal University,2012. DOI:    10.7666/d.y2148260.
[20]McFadden L M, Paris J J, Mitzelfelt M S, et al. Sex-dependent effects of chronic unpredictable stress in the water maze[J]. Physiol Behav, 2011, 102(3/4): 266-275. DOI:10.1016/j.physbeh.2010.10.022.
[21]Uysal N, Sisman A R, Dayi A,et al.Acute footshockstress increases spatial learning memory and correlates to increased hippocampal BDNF and VEGF and cell numbers in adolescent male and female rats[J].Neurosci Lett,2012,514(2):141-146.DOI:10. 1016/j.neulet.2012.02.049.
[22]肖婧姗, 李洋, 李娜,等. BDNF在急性应激所致小鼠空间学习记忆功能改变中的作用[J]. 科技视界, 2016(1):56-57. DOI:10.3969/j.issn.20952457.2016.01.044.
Xiao J S,Li Y,Li N,et al. Role of BDNF in the Spatial Learning-memory Function of Mice Induced by Acute Stress [J].Science & Technology Vision,2016(1):56-57. DOI:10.3969/j.issn.20952457.2016.01.044.
[23]Cazakoff B N, Johnson K J, Howland J G. Converging effects of acute stress on spatial and recognition memory in rodents: a review of recent behavioural and pharmacological findings[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2010, 34(5): 733-741. DOI:10.1016/j.pnpbp.2010.04.002.
[24]Philbert J, Pichat P, Beesk  S, et al. Acute inescapable stress exposure induces longterm sleep disturbances and avoidance behavior: a mouse model of posttraumatic stress disorder (PTSD)[J]. Behav Brain Res, 2011, 221(1): 149-154. DOI:10.1016/j.bbr.2011.02.039.
[25]Sijbrandij M, Kleiboer A, Bisson J I, et al. Corrections. Pharmacological prevention of posttraumatic stress disorder and acute stress disorder: a systematic review and metaanalysis[J]. Lancet Psychiatry, 2015, 2(7): 584. DOI:10.1016/S2215-0366(15)00280-1.
[26]Hansen M, Armour C, Wittmann L, et al. Is there a common pathway to developing ASD and PTSD symptoms?[J]. J Anxiety Disord, 2014, 28(8): 865-872. DOI:10.1016/j.janxdis.2014.09.019.

更新日期/Last Update: 2017-06-07