[1]吴栋栋,徐伦山,许民辉,等.大鼠小胶质细胞及巨噬细胞对胶质瘤C6细胞株迁移能力的影响[J].第三军医大学学报,2017,39(11):1117-1122.
 Wu Dongdong,Xu Lunshan,Xu Minhui,et al.Effects of microglia and macrophages on migration of rat C6 glioma cells[J].J Third Mil Med Univ,2017,39(11):1117-1122.
点击复制

大鼠小胶质细胞及巨噬细胞对胶质瘤C6细胞株迁移能力的影响(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第11期
页码:
1117-1122
栏目:
基础医学
出版日期:
2017-06-15

文章信息/Info

Title:
Effects of microglia and macrophages on migration of rat C6 glioma cells
作者:
吴栋栋徐伦山许民辉欧阳庆易良
第三军医大学大坪医院野战外科研究所神经外科
Author(s):
Wu Dongdong Xu Lunshan Xu Minhui Ouyang Qing Yi Liang

Department of Neurosurgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China

关键词:
小胶质细胞巨噬细胞肿瘤迁移
Keywords:
microglia cells macrophage tumor migration
分类号:
R329.28; R73-37; R730.264
文献标志码:
A
摘要:

R329.28; R73-37; R730.264

Abstract:

Objective      To determine the effect of microglia and peripheral blood macrophages on the migration of C6 glioma cells. Methods      The rat microglial cells and macrophages differentiated from peripheral blood mononuclear cells were co-cultured with C6 glioma cells. Co-cultured with rat fibroblasts served as positive control and singly cultured C6 cells as blank control. Scratch wound healing assay was carried in 0, 24 and 48 h after co-culture, and the results were observed in 6 h later. In 24 h and 48 h after co-culture, CD11b+ flow cytometry was used to sort and isolate microglia/macrophages and C6 cells. The expression levels of IL-10, IL-18 and MMP-9 in microglia and macrophages and those of TGF-β and FAS ligand in glioma cells were detected by Western blotting when co-cultured for 0, 24 and 48 h.  Results       At the initial state, both microglia and macrophages inhibited the migration of glioma, and the inhibitory effect of macrophage was stronger (P<0.05). The tumor inhibitory effect of 2 kinds of cells disappeared after co-culture with glioma C6 cells, and all of them showed the effect of promoting tumor migration. The expression levels of IL-10, IL-18 and MMP-9 in the microglia and macrophages were different in the initial stage. But the levels of these proteins were increased in these cells and consistent after co-culture. The expression levels of TGF-β and FAS ligand were also increased and consistent in glioma cells C6 after co-culture. Conclusion      Microglia and macrophages are domesticated into the same glioma associated microglia/macrophages after co-culture with C6, and 2 kinds of cells show the same biological effects when co-culture with C6 cells.

参考文献/References:

[1]王忠诚. 王忠诚神经外科学[M]. 武汉:湖北科学技术出版社, 2005:1-1130.
Wang Z C. The Department of Neurosurgery[M].Wuhan:Hubei Science and Technology Press, 2005:1-1130.
[2]Davis F G, Freels S, Grutsch J, et al. Survival rates in patients with primary malignant brain tumors stratified by patient age and tumor histological type: an analysis based on Surveillance, Epidemiology, and End Results (SEER) data, 1973-1991[J]. J Neurosurg, 1998, 88(1): 1-10. DOI:10.3171/jns.1998.88.1.0001.
[3]王平,郑芳,李炜修,等.宫颈癌发病年龄及病因变化趋势研究[J].现代肿瘤医学,2007,15(12):1835-1837. DOI:10.3969/j.issn.1672-4992.2007.12.050.
Wang P,Zheng F,Li W X,et al. The trend of age and etiological changes in cervical carcioma[J].Modern Oncology,2007,15(12):1835-1837.DOI:10.3969/j.issn.1672-4992.2007.12.050.
[4]Penfield W. Microglia and the Process of Phagocytosis in Gliomas[J]. Am J Pathol, 1925, 1(1): 77-90.
[5]AlShakweer W, Alwelaie Y, Mankung A M, et al. Bone marrowderived microglia in pilocytic astrocytoma[J]. Front Biosci (Elite Ed), 2011, 3: 371-379. DOI:10.2741/e252.
[6]李锐,郭民侠,李晓青,等.不同分离纯化法原代培养小胶质细胞的生物学差异[J].细胞与分子免疫学杂志,2009,25(4):366-368. DOI:10.3321/j.issn:1007-8738.2009.04.026.
Li R, Guo M X, Li X Q,et al. Biological differences of primary cultured microglia by different methods of separation and purification [J]. Chin J Cell Mol Immunol, 2009,25(4):366-368.DOI:10.3321/j.issn:1007-8738.2009.04.026.
[7]郑志竑, 林玲. 神经细胞培养理论与实践[M]. 北京:科学出版社, 2002:1-250.
Zheng Z H,Lin L. The theory and practice of neural cell culture[M].Beijing: Scientific Press, 2002:1-250.
[8]Saura J, Tusell J M, Serratosa J. Highyield isolation of murine microglia by mild trypsinization[J]. Glia, 2003, 44(3): 183-189. DOI:10.1002/glia.10274.
[9]Coniglio S J, Eugenin E, Dobrenis K, et al. Microglial stimulation of glioblastoma invasion involves epidermal growth factor receptor (EGFR) and colony stimulating factor 1 receptor (CSF1R) signaling[J]. Mol Med, 2012, 18(1): 519-527. DOI:10.2119/molmed.2011.00217.
[10]Yeh W L, Lu D Y, Liou H C, et al. A forward loop between glioma and microglia: glioma-derived extracellular matrixactivated microglia secrete IL-18 to enhance the migration of glioma cells[J]. J Cell Physiol, 2012, 227(2): 558-568. DOI:10.1002/jcp.22746.
[11]赵红宇,金辉,于宏伟,等.基质金属蛋白酶对胶质瘤干细胞侵袭作用的影响[J].中国肿瘤临床,2009,36(7):388-392. DOI:10.3969/j.issn.10008179.2009.07.009.
Zhao H Y,Jin H,Yu H W,et al. The effects of cancer stem cells on glioma invasion mechanism through matrix metallopro-teinasesl[J]. Chinese Journal of Clinical Oncology, 2009, 36(7):388-392.DOI:10.3969/j.issn.1000-8179.2009.07.009.
[12]Jansen T, Tyler B, Mankowski J L, et al. FasL gene knock-down therapy enhances the antiglioma immune response[J]. Neuro Oncol, 2010, 12(5): 482-489. DOI:10.1093/neuonc/nop052.
[13]Voisin P, Bouchaud V, Merle M, et al. Microglia in close vicinity of glioma cells: correlation between phenotype and metabolic alterations[J]. Front Neuroenergetics, 2010, 2: 131. DOI:10.3389/fnene.2010.00131.
[14]Zhang L, Liu W, Alizadeh D, et al. S100B attenuates microglia activation in gliomas: possible role of STAT3 pathway[J]. Glia, 2011, 59(3): 486-498. DOI:10.1002/glia.21118.
[15]Jacobs V L, Landry R P, Liu Y, et al. Propentofylline decreases tumor growth in a rodent model of glioblastoma multiforme by a direct mechanism on microglia[J]. Neuro Oncol,2012,14(2): 119-131. DOI:10.1093/neuonc/nor194.
[16]Yi L, Xiao H, Xu M, et al. Glioma-initiating cells: a predominant role in microglia/macrophages tropism to glioma[J]. J Neuroimmunol, 2011, 232(1/2): 75-82. DOI:10.1016/j.jneuroim.2010.10.011.
[17]Komohara Y, Ohnishi K, Kuratsu J, et al. Possible involve-ment of the M2 anti-inflammatory macrophage phenotype in growth of human gliomas[J]. J Pathol, 2008, 216(1): 15-24. DOI:10.1002/path.2370.
[18]周长胜,丁鹏,王崇谦,等.胶质瘤组织肿瘤相关巨噬细胞表型检测临床意义分析[J].中华肿瘤防治杂志,2013,20(11):845-849.
Zhou C S,Ding P,Wang C Q,et al. Detection of tumor-associated macrophage phenotype in glioma and its significancel[J]. Chin J cancer prev treat,2013,20(11):845-849.
[19]Pyonteck S M, Akkari L, Schuhmacher A J, et al. CSF-1R inhibition alters macrophage polarization and blocks glioma progression[J].Nat Med,2013,19(10):1264-1272.DOI:10.1038/nm.3337.

相似文献/References:

[1]任宁,周欣,李贺,等.巨噬细胞移植对大鼠心肌梗死后细胞外基质修复的影响[J].第三军医大学学报,2012,34(17):1727.
 Ren Ning,Zhou Xin,Li He,et al.Effect of macrophage transplantation on extracellular matrix repair after myocardial infarction in rats[J].J Third Mil Med Univ,2012,34(11):1727.
[2]李明,孙守兵,周越,等.去甲肾上腺素诱导人巨噬细胞MMP-9的表达及机制[J].第三军医大学学报,2012,34(17):1758.
 Li Ming,Sun Shoubing,Zhou Yue,et al.Norepinephrine induces expression of matrix metalloproteinase-9 in human macrophages and its mechanisms[J].J Third Mil Med Univ,2012,34(11):1758.
[3]任泂,粟永萍,李洪涛,等.JAB1表达下调对LPS诱导炎性因子TNF-α和IL-6的影响[J].第三军医大学学报,2007,29(16):1552.
 REN Jiong,SU Yong-ping,LI Hong-tao,et al.Effect of JAB1 down-regulation on LPS-induced TNF-α and IL-6 in mouse macrophage cells[J].J Third Mil Med Univ,2007,29(11):1552.
[4]周建云,蒋建新,杨策,等.皮质酮对大鼠腹腔巨噬细胞功能的影响[J].第三军医大学学报,2008,30(02):120.
 ZHOU Jian-yun,JIANG Jian-xin,YANG Ce,et al.Effect of corticosterone on functions of rat peritoneal macrophages[J].J Third Mil Med Univ,2008,30(11):120.
[5]章必成,王俊,赵勇,等.不同活化表型的巨噬细胞对Lewis肺癌细胞增殖和侵袭的影响[J].第三军医大学学报,2007,29(11):1013.
 ZHANG Bi-cheng,WANG Jun,ZHAO Yong,et al.Effect of macrophages with different activated phenotype on proliferation and invasion of Lewis lung carcinoma cells[J].J Third Mil Med Univ,2007,29(11):1013.
[6]曹国强,钱桂生,谢志坚.慢性阻塞性肺病患者肺泡巨噬细胞凋亡测定及其意义[J].第三军医大学学报,2005,27(17):1796.
[7]许文,王阁,陈川,等.索拉菲尼诱导大鼠肝细胞癌形成过程中巨噬细胞和自然杀伤细胞的聚集[J].第三军医大学学报,2009,31(11):1009.
 XU Wen,WANG Ge,CHEN Chuan,et al.Sorafenib induces aggregation of macrophages and natural killer cells in hepatocellular carcinoma rats[J].J Third Mil Med Univ,2009,31(11):1009.
[8]裴轶劲,吴小兰,毛方圆,等.N-乙酰氨基葡萄糖对应激小鼠巨噬细胞吞噬功能的调节作用[J].第三军医大学学报,2009,31(19):1869.
 PEI Yi-jin,WU Xiao-lan,MAO Fang-yuan,et al.Regulation of N-Acetyl-D-glucosamine on macrophages function of stressed mice[J].J Third Mil Med Univ,2009,31(11):1869.
[9]周建云,周琳琳,严军,等.去甲肾上腺素对脂多糖诱导的巨噬细胞活化的影响[J].第三军医大学学报,2009,31(13):1278.
 ZHOU Jian-yun,ZHOU Lin-lin,YAN Jun,et al.Effects of norepinephrine on the activation of LPS-induced macrophages[J].J Third Mil Med Univ,2009,31(11):1278.
[10]卓燕,余荣杰,赵洪雯,等.阿托伐他汀抑制ApoE-/-小鼠腹腔巨噬细胞LPS刺激下NF-κB p65核转位[J].第三军医大学学报,2011,33(20):2120.
 Zhuo Yan,Yu Rongjie,Zhou Hongwen,et al.Atorvastatin suppresses nuclear translocation of NF-κB p65 in LPS-induced abdominal macrophage of ApoE-/- mouse[J].J Third Mil Med Univ,2011,33(11):2120.

更新日期/Last Update: 2017-06-07