[1]马廷廷,黄延风,杨震华,等.儿童结核病结核分枝杆菌临床分离株中esxA、fbpB、Rv2660c基因多态性研究[J].第三军医大学学报,2017,39(17):1768-1773.
 MA Tingting,HUANG Yanfeng,YANG Zhenhua,et al.Gene polymorphisms of esxA, esxH and fbpB in clinical Mycobacterium tuberculosis isolates from tuberculosis children[J].J Third Mil Med Univ,2017,39(17):1768-1773.
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儿童结核病结核分枝杆菌临床分离株中esxA、fbpB、Rv2660c基因多态性研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第17期
页码:
1768-1773
栏目:
临床医学
出版日期:
2017-09-15

文章信息/Info

Title:
Gene polymorphisms of esxA, esxH and fbpB in clinical Mycobacterium tuberculosis isolates from tuberculosis children
作者:
马廷廷黄延风杨震华苏薇
重庆医科大学附属儿童医院感染科,儿童发育疾病研究教育部重点实验室,儿童发育重大疾病国家国际科技合作基地,儿科学重庆市重点实验室;Department of Epidemiology, School of Public Health, University of Michigan, 1415 Washington Heights, Ann Arbor, MI 481092029, USA
Author(s):
MA Tingting HUANG Yanfeng YANG Zhenhua SU Wei

Department of Infectious Disease, Key Laboratory of Child Development and Disorders of Ministry of Education, International Science and Technology Cooperation Center for Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Children’s Hospital of Chongqing Medical University, Chongqing, 400014, China; Department of Epidemiology, School of Public Health, University of Michigan, MI 481092029, USA

关键词:
结核分枝杆菌esxAfbpBRv2660c基因多态性
Keywords:
Mycobacterium tuberculosis esxA fbpB Rv2660c gene polymorphism
分类号:
R738.911; R394.3; R529.9
文献标志码:
A
摘要:

目的      对抗原编码基因esxA、fbpB、Rv2660c进行测序,了解esxA、fbpB、Rv2660c的基因多态性。方法     选取135株结核分枝杆菌(Mycobacterium tuberculosis,MTB)临床分离株,运用PCR方法扩增目的基因esxA、fbpB、Rv2660c并进行测序,与结核标准株H37Rv基因序列以及从免疫表位数据库(immune epitope database,IEDB)中查询到的表位序列进行对比,分析esxA、fbpB、Rv2660c基因序列中的变异特征。结果    在135株菌株中,esxA、Rv2660c序列暂未发现基因变异。fbpB共66株(4889%)发现了基因变异,5个单核苷酸多态性(single nucleotide polymorphism,SNP) 位点共造成8个(14.81%)T细胞抗原表位以及9个(24.32%)B细胞抗原表位序列的多态性。fbpB基因的dN值为0.000 120,dS为0.002 126,其中T细胞表位区与非T细胞表位区的dN值分别为0.000 067和0.000 408,dS分别为0.002 382和0.000 470,B细胞表位区dN和dS值分别为0.000 063和0.002 380,非B细胞表位区dN和dS均为0。结论     esxA、Rv2660c基因序列较保守,fbpB存在基因多态性,预测会对蛋白Ag85B的免疫功能造成影响,从而影响H56的免疫有效性。

Abstract:

Objective     To sequence the antigen-encoding genes esxA, fbpB and Rv2660c from clinical isolates of Mycobacterium tuberculosis (MTB) in tuberculosis children and study the polymorphisms. Methods     A total of 135 isolated strains were amplified for gene sequences of esxA, fbpB and Rv2660c by PCR, and the products were sequenced respectively. The results of sequencing were compared with the corresponding gene sequences of standard strain H37Rv and human T and B cell epitope sequences from immune epitope database (IEDB). The genetic diversities of the gene sequences of esxA, fbpB and Rv2660c were analyzed. Results     Among the 135 strains, no gene polymorphisms were found in esxA and Rv2660c. But fbpB had genetic diversities in 66 clinical isolates (48.89%). The 5 single nucleotide polymorphism (SNP) mutations in fbpB affected 8 (14.81%) T cell epitopes and 9 (24.32%) B cell epitopes. The dN and dS values of whole fbpB gene were 0.000 120 and 0.002 126, respectively. The dN and dS values of T-epitopes areas were 0.000 067 and 0.002 382 respectively, those of non-T epitopes area were 0.000 408 and 0.000 470, respectively, those of B epitopes areas were 0.000 063 and 0.00 2380, and those of non-B epitopes areas were 0. Conclusion     The gene sequences of esxA and Rv2660c are conservative, and that of fbpB contains genetic diversities. Its polymorphisms may influence the function of antigen Ag85B, and thus affect the efficacy of vaccine H56.

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更新日期/Last Update: 2017-09-05