[1]夏丽琴,冯忠明,陈旭,等.网络筛选乳腺癌相关miRNAs及miR-106a-5p对乳腺癌细胞侵袭迁移能力的影响[J].第三军医大学学报,2017,39(02):130-136.
 Xia Liqin,Feng Zhongming,Chen Xu,et al.Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells[J].J Third Mil Med Univ,2017,39(02):130-136.
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网络筛选乳腺癌相关miRNAs及miR-106a-5p对乳腺癌细胞侵袭迁移能力的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第02期
页码:
130-136
栏目:
基础医学
出版日期:
2017-01-30

文章信息/Info

Title:
Screening of key miRNAs related with breast cancer by bioinformatics network and effect of miR-106a-5p on invasion and migration in breast cancer cells
作者:
夏丽琴冯忠明陈旭李妍王艳梅卢丁烯古骄阳孙建国
第三军医大学新桥医院肿瘤科
Author(s):
Xia Liqin Feng Zhongming Chen Xu Li Yan Wang Yanmei Lu Dingxi Gu Jiaoyang Sun Jianguo

Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China

关键词:
miR-106a-5p生物信息学乳腺癌细胞侵袭迁移
Keywords:
miR-106a-5p bioinformatics analysis breast cancer cells invasion migration 
分类号:
R394-33;R394.3;R737.9
文献标志码:
A
摘要:

目的     网络筛选乳腺癌关键微小RNAs(microRNAs,miRNAs),并探讨miR-106a-5p对乳腺癌细胞侵袭及迁移能力的影响。方法      通过TargetScan和miRanda等工具,构建miRNAs与靶基因以及显著靶向性功能(gene ontology,GO)的调控网络,筛选对乳腺癌有关键调控作用的miRNAs;利用miR-106a-5p agomir及miR106a-5p antagomir转染MCF-7、T47D乳腺癌细胞株,建立乳腺癌细胞中miR-106a-5p的过表达及敲低体系,Transwell侵袭实验、Transwell迁移实验、划痕实验检测miR-106a-5p对乳腺癌细胞的侵袭、迁移能力的影响。结果     在miRNAs-gene-network和miRNAs-GO-network两个网络中调控维度最高的miRNAs基本一致,其中miR-106a-5p是调控维度最高的miRNAs之一;过表达miR-106a-5p显著抑制乳腺癌细胞的侵袭能力和迁移能力(P<0.05),敲低miR-106a-5p则显著增加乳腺癌细胞的侵袭能力和迁移能力(P<0.05)。结论     miR-106a-5p是乳腺癌miRNAs调控网络中的关键miRNAs之一,具有抑制乳腺癌细胞的侵袭及迁移能力的生物学功能。

Abstract:

Objective     To screen key miRNAs related with breast cancer and explore the modulating effect of miR106a-5p on the invasion and migration of breast cancer cells. Methods     TargetScan and miRanda softwares were employed to predict the targets of miRNA array in breast cancer cells. The miRNAsgene-network and miRNAs-GO-network were established based on gene ontology (GO) analysis. The overexpression and knockdown of miR-106a-5p were established by transfection of miR-106a-5p agomir and miR-106a-5p antagomir into MCF-7 and T47D breast cancer cells, respectively. The invasion and migration of breast cancer cells were tested by Transwell invasion assay, Transwell migration assay and scratch assay. Results     MiR-106a-5p was one of miRNAs with the highest regulating degree in both miRNAs-gene-network and miRNAs-GO-network. The expression of miR-106a-5p in breast cancer cells was significantly lower than that in normal mammary cells (P<0.05). The overexpression of miR-106a-5p inhibited the invasion and migration of MCF-7 and T47D breast cancer cells (P<0.05). On the contrary, the knockdown of miR-106a-5p promoted the invasion and migration of breast cancer cells (P<0.05). Conclusion     miR-106a-5p is a key miRNA in the regulation network of breast cancer miRNAs, and can inhibit the invasion and migration of breast cancer cells.

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更新日期/Last Update: 2017-01-18