[1]曹令平,涂利宽,吴小翎.西奥骨化醇抑制TGF-β1诱导的肝癌细胞SMMC-7721上皮-间质转化[J].第三军医大学学报,2017,39(01):42-47.
 Cao Lingping,Tu Likuan,Wu Xiaoling.Seocalcitol inhibits transforming growth factor-β1-induced epithelialmesenchymal transition in hepatoma carcinoma SMMC-7721 cells[J].J Third Mil Med Univ,2017,39(01):42-47.
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西奥骨化醇抑制TGF-β1诱导的肝癌细胞SMMC-7721上皮-间质转化(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
39卷
期数:
2017年第01期
页码:
42-47
栏目:
基础医学
出版日期:
2017-01-15

文章信息/Info

Title:
Seocalcitol inhibits transforming growth factor-β1-induced epithelialmesenchymal transition in hepatoma carcinoma SMMC-7721 cells
作者:
曹令平涂利宽 吴小翎
重庆医科大学附属第二医院消化内科
Author(s):
Cao Lingping Tu Likuan Wu Xiaoling

Department of Gastroenterology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

关键词:
EB1089上皮-间质转化转化生长因子&beta1肝癌
Keywords:
EB1089 epithelial-mesenchymal transition transforming growth factor -&beta1hepatic carcinoma
分类号:
R730.23; R735.7; R977.24
文献标志码:
A
摘要:

目的     研究西奥骨化醇(seocalcitol,EB1089)对TGF-β1诱导肝癌细胞SMMC-7721上皮-间质转化(epithelial-mesenchymal transitions,EMT)的抑制作用。方法     用不同浓度维生素D类似物EB1089(1、10、100、1 000 nmol/L)作用于肝癌细胞SMMC-7721 6、12、24、48 h,筛选出具有统计学意义的作用浓度和时间。将肝癌细胞SMMC-7721分为4组(EB1089组、TGF-β1组、EB1089+ TGF-β1组和空白对照组),分别干预48 h。用相差倒置显微镜观察细胞形态变化;通过实时RT-PCR和Western blot分别检测E-cadherin、N-cadherin、Vimentin的mRNA和蛋白表达情况;迁移实验和侵袭实验检测EB1089对肝癌细胞SMMC-7721侵袭和迁移能力的影响。结果     TGF-β1作用后肝癌细胞SMMC-7721呈现长梭形,并伸出较多触角,EB1089能明显改善这种形态变化;EB1089可有效升高TGF-β1所致的E-cadherin mRNA和蛋白低表达(P<0.05),同时降低N-cadherin、Vimentin mRNA和蛋白表达(P<0.05);EB1089对TGF-β1诱导的细胞侵袭、迁移具有抑制作用(P<0.05)。结论     维生素D类似物EB1089能有效抑制TGF-β1诱导的肝癌细胞SMMC-7721上皮-间质转化现象和侵袭、迁移能力。

Abstract:

Objective      To determine the inhibitory effect of seocalcitol (EB1089) on transforming growth factorβ1 (TGF-β1)induced epithelial-mesenchymal transition (EMT) in the hepatoma carcinoma cell line SMMC-7721. Methods     Vitamin D analog EB1089 at different concentrations (1, 10, 100 and 1 000 nmol/L) was used to treat the SMMC-7721 cells for different times (6, 12, 24 and 48 h) to determine the optimal dose and time. Then the SMMC-7721 cells were divided into control group, TGF-β1 group, EB1089 with or without TGF-β1 groups. Cell morphology was observed with phase-contrast microscopy. RT-PCR and Western blotting were employed to measure the expression of E-cadherin, N-caherin and Vimentin at mRNA and protein levels. Cell invasion and migration were evaluated by Transwell chamber assay. Results      Compared with control cells, TGF-β1 stimulated cells developed an obvious long fusiform shape. Such morphological changes were prevented by the addition of EB1089. Meanwhile, EB1089 enhanced the protein and mRNA expression of E-cadherin suppressed by TGF-β1 (P<0.05), decreased the levels of N-caherin and Vimentin (P<0.05), and inhibited TGF-β1-induced invasiveness and migration in the SMMC-7721 cells. Conclusion      Vitamin D analog EB1089 inhibits TGF-β1-induced EMT, and invasion and migration in the hepatoma carcinoma SMMC-7721 cells.

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更新日期/Last Update: 2017-01-02