[1]李德长,苏会岚,糜漫天,等.Pt@GR复合物用于构建高灵敏电化学甲胎蛋白传感器的研究[J].第三军医大学学报,2016,38(22):2444-2449.
 Li Dezhang,Su Huilan,Mi Mantian,et al.Pt@GR complex-based electrochemical immunosensor for AFP detection[J].J Third Mil Med Univ,2016,38(22):2444-2449.
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Pt@GR复合物用于构建高灵敏电化学甲胎蛋白传感器的研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第22期
页码:
2444-2449
栏目:
基础医学
出版日期:
2016-11-30

文章信息/Info

Title:
Pt@GR complex-based electrochemical immunosensor for AFP detection
作者:
李德长苏会岚 糜漫天张瑞林
成都医学院公共卫生系;第三军医大学军事预防医学院营养与食品卫生学教研室
Author(s):
Li Dezhang Su Huilan Mi MantianZhang Ruilin

Department of Public Health, Chengdu Medical College, Chengdu, Sichuan Province, 610500; Department of Nutrition and Food Hygiene, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038,China

关键词:
石墨烯纳米铂复合纳米材料电化学免疫传感器甲胎蛋白
Keywords:
graphene platinum hybrid nanomaterial electrochemical immunosensor alphafetoprotein
分类号:
R319;R39433;R446
文献标志码:
A
摘要:

目的       构建基于铂与石墨烯纳米复合物(Pt@GR)的电化学免疫传感器。方法       在洁净的玻碳电极表面滴涂一层壳聚糖与Pt@GR的复合物,通过共价作用将纳米金固定在电极表面,进一步固载甲胎蛋白抗体制得甲胎蛋白免疫传感器,采用循环伏安法(cyclic voltammetry, CV)、示差脉冲伏安法(differential pulse voltammetry, DPV)对实验条件进行优化,对该传感器的响应特性进行研究。同时制备基于普鲁士蓝和石墨烯的免疫传感器以对照研究不同传感器的性能特点。结果        免疫反应最佳时间为24 min,测试底液最佳pH为6.5。在最优的实验条件下,连续扫描100圈,其电流响应下降5.1%。平行测定5支电极的RSD为 8.1%,加标回收率为 96.40%~102.49%。在测试底液中加入过氧化氢,其线性范围为0.1~100.0 ng/mL,检测限为0.026 ng/mL。结论        基于Pt@GR复合物修饰的电化学传感器具有灵敏度高和稳定性好的特点,可用于甲胎蛋白的高灵敏检测。 

Abstract:

Objective     To fabricate Pt@GR nanocomposite-based electrochemical immunosensor for detecting alphafetoprotein (AFP) with high sensitivity.  Methods      The proposed immunosensor was prepared firstly by using the Pt@GR hybrid self-assembled onto the electrode surface. Then gold nanoparticles were immobilized to the surface of modified electrode via covalent-bonding interaction. Finally, antibody could be absorbed to the electrode by specific binding to antigen. The experimental conditions was optimized by methods of cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The effects of experimental parameters and performance of the immunosensor such as reproducibility, sensitivity and stability were investigated. In addition, characteristics of the Pt@GR based immunosensor was compared with another constructed by coating Prussian blue on the graphene nanosheets .  Results     The optimal immunoreaction time and pH value of the detection solution were 24 min and 6.5, respectively. Under the optimal experimental conditions, the current was decreased by 5.1% after 100 cycles of consecutive scanning. The relative standard deviation (RSD) of 5 electrode-measurements was 8.1%, and the recovery rate of the immunosensor was in the range of 96.4% to 102.49%. After addition of hydrogen peroxide in the detection solution, the prepared immunosensor displayed a liner relationship within the range of 0.1~100 ng/mL with a detection limit of 0.026 ng/mL.  Conclusion     The high sensitivity and stability of Pt@GR complex-based immunosensor is applicable for the detection of AFP with low concentration. 

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更新日期/Last Update: 2016-11-23