[1]吴永梅,杨琴,李淑蓉,等.高浓度葡萄糖对斑马鱼胚胎及多巴胺神经元发育的影响[J].第三军医大学学报,2016,38(15):1750-1754.
 Wu Yongmei,Yang Qin,Li Shurong,et al.Impact of high concentration of glucose on development of zebrafish embryos and dopaminergic neurons[J].J Third Mil Med Univ,2016,38(15):1750-1754.
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高浓度葡萄糖对斑马鱼胚胎及多巴胺神经元发育的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第15期
页码:
1750-1754
栏目:
基础医学
出版日期:
2016-08-15

文章信息/Info

Title:
Impact of high concentration of glucose on development of zebrafish embryos and dopaminergic neurons
作者:
吴永梅杨琴李淑蓉苏炳银
成都医学院人体解剖与组织胚胎学教研室,发育与再生四川省重点实验室
Author(s):
Wu Yongmei Yang Qin Li Shurong Su Bingyin

Department of Anatomy, Histology and Embryology, Sichuan Key Laboratory of Development and Regeneration, Chengdu Medical College, Chengdu, Sichuan Province, 610500, China

关键词:
葡萄糖斑马鱼多巴胺神经元
Keywords:
glucose zebrafish dopamine
分类号:
R532.3; Q954.4; Q954.671
文献标志码:
A
摘要:

目的      研究高浓度葡萄糖在斑马鱼胚胎早期发育中的作用以及对斑马鱼多巴胺神经元发育的影响。      方法      实验分为3组:空白对照组,葡萄糖实验(25 mmol/L葡萄糖)组,甘露醇对照(25 mmol/L甘露醇)组。25 mmol/L葡萄糖溶液处理Vmat-GFP转基因斑马鱼胚胎,当胚胎发育到第3天时,激光共聚焦显微镜观察多巴胺神经元的发育,荧光定量PCR检测PD相关基因的表达。      结果      与空白对照组、甘露醇组相比,25 mmol/L葡萄糖溶液处理胚胎后,胚胎发育迟缓,心脏畸形,多巴胺神经元荧光增强,端脑部位多巴胺神经元数量增加,PD(帕金森病)相关基因表达水平升高,其中parkin基因表达差异具有统计学意义(P<0.05)。      结论      25 mmol/L葡萄糖溶液对胚胎的发育具有毒性作用,但在胚胎发育的早期对多巴胺神经元具有营养作用。

Abstract:

Objective      To study the effect of high concentration of glucose on the early development of embryos and dopaminergic neurons in zebrafish.       Methods      Vmat-GFP transgenic zebrafish was used. After fertilization, the embryos were divided into control, mannitol group (25 mmol/L) and glucose group (25 mmol/L). In 3 d after fertilization, the morphology of embryonic dopaminergic neurons was visualized under laser scanning confocal microscope (LSCM). The expression of PD (Parkinson’s disease)-related genes was measured using fluorescent quantitative polymerase chain reaction (PCR).       Results      Compared with control and mannitol group, the embryonic development in glucose group was retarded with cardiac abnormalities. Enhanced fluorescent intensity of Vmat-GFP and increased number of dopaminergic neurons in the telencephalon were also observed. In glucose group the expression of PD-related genes was upregulated, and particularly, upregulation of PARKIN was significant (P<0.05).       Conclusion      High concentration of glucose (25 mmol/L) is toxic to embryonic development, but neurotrophic for dopaminergic neurons at the early stage of embryos in zebrafish.

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更新日期/Last Update: 2016-07-21