[1]康琳,高姗,周阳,等.3种方法对鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌的鉴定[J].第三军医大学学报,2016,38(11):1205-1209.
 Kang Lin,Gao Shan,Zhou Yang,et al.Identification of Burkholderia mallei and Burkholderia pseudomallei by 3 methods[J].J Third Mil Med Univ,2016,38(11):1205-1209.
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3种方法对鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌的鉴定(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第11期
页码:
1205-1209
栏目:
专题报道
出版日期:
2016-06-15

文章信息/Info

Title:
Identification of Burkholderia mallei and Burkholderia pseudomallei by 3 methods
作者:
康琳高姗周阳辛文文杨浩方瑶毛旭虎王景林
军事医学科学院微生物流行病学研究所,病原微生物生物安全国家重点实验室;第三军医大学西南医院医学检验系临床微生物及免疫学教研室
Author(s):
Kang Lin Gao Shan Zhou Yang Xin Wenwen Yang Hao Fang Yao Mao Xuhu Wang Jinglin

State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, 100071; Department of Clinical Microbiology and Immunology, Faculty of Medical Laboratory Sciences, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China

关键词:
鼻疽伯克霍尔德菌 类鼻疽伯克霍尔德菌 MALDI-TOF-MSFQ-PCR16S rRNA基因序列分析
Keywords:
Burkholderia mallei Burkholderia pseudomallei MALDI-TOF-MS FQ-PCR 16S rRNA gene sequencing
分类号:
R378; R446.5
文献标志码:
A
摘要:

目的      用3种检测方法对同源性较高的鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌进行鉴定,比较该3种检测方法的鉴定效果。      方法      分别利用基于MALDI-TOF-MS的蛋白指纹图谱方法、双重荧光定量PCR方法和16S rRNA基因序列分析方法对5株鼻疽伯克霍尔德菌和5株类鼻疽伯克霍尔德菌进行鉴定,综合判断鉴定结果,并与传统的形态学和生化鉴定结果比较,寻找更适合于该两种菌的鉴定方法。      结果      在10株受试菌株中,2株排除是鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌的可能,剩余8株之中,MALDI-TOF-MS方法正确鉴定了6株,双重荧光定量PCR方法正确鉴定了8株,16S rRNA基因序列分析方法正确鉴定4株。      结论      MALDI-TOF-MS的蛋白指纹图谱方法和双重荧光定量PCR方法对鼻疽伯克霍尔德菌和类鼻疽伯克霍尔德菌具有很好的鉴定能力,16S rRNA基因序列分析方法不适合用于该2种菌的鉴定。

Abstract:

Objective      To identify Burkholderia mallei and Burkholderia pseudomallei with 3 methods.       Methods      MALDI-TOF-MS, FQ-PCR and 16S rRNA gene sequencing were used to identify 10 strains which may be Burkholderia mallei or Burkholderia pseudomallei, and the results were compared with those of traditional morphological and biochemical methods so as to select reliable method for identifying Burkholderia mallei and Burkholderia pseudomallei.       Results      Two strains were identified to neither Burkholderia mallei nor Burkholderia pseudomallei. In the rest of 8 strains, 6 strains were correctly identified by MALDI-TOF-MS, 8 strains were correctly identified by FQ-PCR, and 4 strains were correctly identified by 16S rRNA gene sequencing.       Conclusion      MALDI-TOF-MS and FQ-PCR are more suitable for identifying Burkholderia mallei and Burkholderia pseudomallei than 16S rRNA gene sequencing.

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更新日期/Last Update: 2016-05-28