[1]何乐,杨宇馨,张诗珩,等.血清APE1自身抗体在结直肠癌诊断中的价值[J].第三军医大学学报,2016,38(11):1297-1301.
 He Le,Yang Yuxin,Zhang Shiheng,et al.Diagnostic value of serum APE1 autoantibodies in colorectal cancer[J].J Third Mil Med Univ,2016,38(11):1297-1301.
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血清APE1自身抗体在结直肠癌诊断中的价值(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第11期
页码:
1297-1301
栏目:
临床医学
出版日期:
2016-06-15

文章信息/Info

Title:
Diagnostic value of serum APE1 autoantibodies in colorectal cancer
作者:
何乐杨宇馨张诗珩罗佳戴楠李梦侠单锦露王宏毅王东
第三军医大学大坪医院野战外科研究所肿瘤中心;华博特生物科技有限公司
Author(s):
He Le Yang Yuxin Zhang Shiheng Luo Jia Dai Nan Li Mengxia Shan Jinlu Wang Hongyi Wang Dong

Cancer Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042; HUABOTE Biotechnological Inc., Tianjin, 300350, China

关键词:
APE1自身抗体结直肠癌肿瘤标志物
Keywords:
APE1-AAbs colorectal cancer tumor marker
分类号:
R392.11; R730.43; R735.3
文献标志码:
A
摘要:

目的      探讨血清中APE1自身抗体(apurinic/apyrimidinic endonuclease 1 autoantibodies,APE1-AAbs)在结直肠癌中的诊断价值。      方法      利用ELISA检测150例结直肠癌患者和170例健康体检人员(对照组)血清APE1-AAbs水平,采用蛋白芯片法检测相应的CEA、CA199和CA242浓度。运用Logistic回归和ROC曲线分析方法,将APE1-AAbs与CEA、CA199及CA242联合分析。      结果      150例结直肠患者血清APE1-AAbs中位水平为2.697,显著高于对照组(P<0.001)。绘制ROC曲线,其AUC为0.797,灵敏度为62.67%,特异性为85.29%。APE1-AAbs分别与CEA、CA199及CA242联合后,AUC、诊断灵敏度和准确性均有所升高。      结论      血清APE1-AAbs对结直肠癌有辅助诊断价值,与临床上常用的结直肠癌肿瘤标志物联用可提高结直肠癌的诊出率。

Abstract:

Objective      To explore the diagnostic value of serum apurinic/apyrimidinic endonuclease 1 autoantibodies (APE1-AAbs) in screening colorectal cancer.       Methods      Serum APE1-AAbs of 150 patients with colorectal cancer and 170 healthy controls were detected by ELISA. Carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA199) and carbohydrate antigen 242 (CA242) were detected by protein chip technology. Logistic regression and receiver operative characteristic (ROC) curve were employed for combined analysis of APE1-AAbs with CEA, CA199 or CA242.       Results      The median level of serum APE1-AAbs in the patients with colorectal cancer was 2.697, which was higher than that of the healthy controls (P<0.001). With the ROC curve, area under curve (AUC) was 0.797, and the sensitivity and specificity were 62.67% and 85.29%, respectively. The AUC, diagnosis sensitivity and accuracy of colorectal cancer were increased when combining serum APE1-AAbs with CEA, CA199 or CA242.       Conclusion      Serum APE1-AAbs can help to diagnose colorectal cancer, and its combination with CEA, CA199 or CA242 can improve the early diagnosis accuracy of colorectal cancer.

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更新日期/Last Update: 2016-05-29