[1]李素铠,欧阳斌,徐晓秋,等.PLGA纳米药物递送系统的建立及其对bFGF生物学活性的维持[J].第三军医大学学报,2016,38(11):1319-1324.
 Li Sukai,Ouyang Bin,Xu Xiaoqiu,et al.Establishment of poly(DL-lactide-co-glycolide) nanoparticle drug delivery system and its maintenance for bioactivity of basic fibroblast growth factor[J].J Third Mil Med Univ,2016,38(11):1319-1324.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第11期
页码:
1319-1324
栏目:
药学
出版日期:
2016-06-15

文章信息/Info

Title:
Establishment of poly(DL-lactide-co-glycolide) nanoparticle drug delivery system and its maintenance for bioactivity of basic fibroblast growth factor
作者:
李素铠欧阳斌徐晓秋李培甘翼博王利元涂兵周强
第三军医大学:西南医院骨科,全军矫形外科中心,药学院药剂学教研室
Author(s):
Li Sukai Ouyang Bin Xu Xiaoqiu Li Pei Gan Yibo Wang Liyuan Tu Bing Zhou Qiang

Department of Orthopaedics, Center of Orthopaedic Surgery, Southwest Hospital,Department of Pharmaceutics, College of Pharmacy, Third Military Medical University, Chongqing, 400038, China

关键词:
聚(乳酸乙醇酸)牛血清白蛋白纳米粒碱性成纤维细胞生长因子缓释
Keywords:
poly(DL-lactide-co-glycolide) bovine serum albumin nanoparticle basic fibroblast growth factor sustained release
分类号:
R912; R945; R977.6
文献标志码:
A
摘要:

目的      探索聚(乳酸乙醇酸)[poly(DL-lactide-co-glycolide) ,PLGA]纳米粒的制备条件,并进一步验证该纳米粒对碱性成纤维细胞因子(basic fibroblast growth factor, bFGF)的负载效率和活性维持。      方法      采用复乳法制备牛血清白蛋白(bovine serum albumin, BSA)的PLGA纳米粒,研究PLGA浓度、PLGA/BSA质量比、水油体积比及内水相溶剂对纳米粒粒径、蛋白包封效率的影响,然后选择优化条件制备负载bFGF的PLGA纳米粒,通过细胞增殖实验验证bFGF的活性。      结果      采用优化条件制备的纳米粒对蛋白的包封率达到70.0%,粒径(373±12) nm,Zeta电位(27.1±0.6) mV。蛋白得以缓慢释放,突释现象不明显。负载bFGF的PLGA纳米粒有较好的活性,对细胞的促增殖作用优于bFGF溶液组。      结论      采用复乳法制备的PLGA纳米粒可用于负载生长因子实现缓释,并能较好地维持其生物学活性。

Abstract:

Objective      To explore the preparation conditions of poly(DL-lactide-co-glycolide) (PLGA) nanoparticles, and validate the entrapment efficiency and activity of basic fibroblast growth factor (bFGF) loaded by PLGA nanoparticles.       Methods      Bovine serum albumin (BSA)-loaded PLGA nanoparticles were prepared with a double emulsion method. First, the effects of the concentration of PLGA, mass ratio of PLGA and BSA, volume ratio of water phase and oil phase, and type of internal water phase on the particle size and entrapment efficiency were studied. Then the optimal conditions were selected to prepare bFGF-loaded PLGA nanoparticles, and the activity of bFGF was validated by proliferation of mesenchymal stem cells (MSCs).       Results      Under the optimal conditions, the entrapment efficiency of BSA-loaded PLGA nanoparticles was 70.0%, the particle size was 373±12 nm, and the Zeta potential was 27.1±0.6 mV. The protein was sustained-released, and the phenomenon of burst release was not obvious. Biological activity of bFGF loaded by PLGA nanoparticles was maintained, and the bFGF-loaded PLGA nanoparticles could better promote the proliferation of MSCs than bFGF solution.       Conclusion      PLGA nanoparticles prepared with the double emulsion method can be used to load growth factors to control their release and maintain their biological activity.

参考文献/References:

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更新日期/Last Update: 2016-05-29