[1]汪冬,杨帆,黄庆愿,等.缺氧对大鼠下丘脑Ⅰ型大麻素受体表达和摄食量的影响[J].第三军医大学学报,2016,38(16):1820-1825.
 Wang Dong,Yang Fan,Huang Qingyuan,et al.Effect of hypoxia on hypothalamic cannabinoid receptor type Ⅰ expression and food intake in rats[J].J Third Mil Med Univ,2016,38(16):1820-1825.
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缺氧对大鼠下丘脑Ⅰ型大麻素受体表达和摄食量的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第16期
页码:
1820-1825
栏目:
基础医学
出版日期:
2016-08-30

文章信息/Info

Title:
Effect of hypoxia on hypothalamic cannabinoid receptor type Ⅰ expression and food intake in rats
作者:
汪冬杨帆黄庆愿周其全
第三军医大学:高原军事医学系高原军事医学地理学教研室,高原军事医学系病理生理学与高原病理学教研室
Author(s):
Wang Dong Yang Fan Huang Qingyuan Zhou Qiquan

Department of High Altitude Disease, Department of Pathophysiology and High Altitude Pathology, College of High Altitude Military Medicine, Third Military Medical University, Chongqing, 400038, China

关键词:
缺氧摄食行为下丘脑c-FosI型大麻素受体
Keywords:
hypoxia food intake hypothalamus c-Fos cannabinoid receptor type
分类号:
R338.24;R341;R364.4
文献标志码:
A
摘要:

目的    研究缺氧大鼠下丘脑Ⅰ型大麻素受体(cannabinoid receptor typeⅠ,CB1)的表达变化及其与摄食量的关系。    方法     54只雄性SD大鼠按随机数字表法分为3组(每组18只):平原对照组、缺氧1 d组和缺氧7 d组。缺氧组大鼠置模拟海拔5 000 m低压舱中,平原对照组大鼠置平原饲养。记录各组大鼠摄食量,蛋白免疫印迹法和免疫组化法观察各组大鼠下丘脑CB1受体和c-Fos蛋白表达变化。    结果      与平原对照组[(15.5±2.7)g]大鼠相比,缺氧1 d组大鼠摄食量[(4.9±0.7)g]显著减少(P<0.05),同时大鼠下丘脑CB1和c-Fos阳性细胞数量和蛋白表达量显著减少;   缺氧1 d组大鼠弓状核(arcuate nucleus, ARC)、下丘脑腹外侧区(later hypothalamic area, LH)和下丘脑腹内侧核(ventromedial hypothalamic nucleus, VMH)CB1受体、c-Fos表达较平原对照组显著降低;   随着缺氧时间的延长,动物摄食量逐日增加,缺氧3~7 d时的摄食量与平原对照组大鼠相当;缺氧7 d组大鼠下丘脑CB1受体和c-Fos阳性细胞数量和蛋白表达量与平原对照组大鼠比较,差异无统计学意义(P>0.05)。   结论       急性缺氧大鼠摄食减少可能与弓状核、下丘脑腹外侧区和下丘脑腹内侧核CB1受体和c-Fos表达减少相关。

Abstract:

Objective      To study the relationship between the hypothalamic expression of cannabinoid receptor type Ⅰ and food intake during hypoxia exposure in rats.       Methods      Adult SD rats were randomly divided into 3 groups: normal group (N), 2day hypoxia exposure group (H1d) and 7day hypoxia exposure group (H7d). The rats of the hypoxia groups were put into a hypobaric chamber simulating 5 000meter high altitude for 1 or 7 d, and the food intake and body weight of each group were recorded. The expression levels of CB1 and c Fos in the hypothalamus were detected by Western blotting and immuohistochemical assay.     Results        After exposure to hypoxia, the food intake of the H1d group was lower than that of the N group significantly (4.9±0.7 vs15.5±2.7 g, P<0.05). The level of CB1 in the arcuate nucleus (ARC), later hypothalamic area (LH) and ventromedial hypothalamic nucleus (VMH) was reduced obviously, and the expression of cFos in the ARC, LH and VMH was decreased, too. The food    intake was increased along with the days of exposure to hypoxia, and in 3~7 d after hypoxia exposure, the rats in the H7d group had the food intake similar with the N group. The levels of CB1 and c-Fos had no significant changes between the H7d group and the N group.    Conclusion       The down regulation of CB1 and c-Fos in the ARC, LH and VMH may be closely associated with the food intake decrease induced by acute exposure to hypoxia.

更新日期/Last Update: 2016-08-15