[1]何雕,张国庆,郑道峰,等.白藜芦醇在缺氧/复氧诱导的肝细胞损伤中的保护作用及与TLR4/NF-κB通路的关系[J].第三军医大学学报,2016,38(12):1398-1403.
 He Diao,Zhang Guoqing,Zheng Daofeng,et al.Resveratrol protects hepatocytes against hypoxia/reoxygenation injury and its relationship with TLR4/NF-κB pathway[J].J Third Mil Med Univ,2016,38(12):1398-1403.
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白藜芦醇在缺氧/复氧诱导的肝细胞损伤中的保护作用及与TLR4/NF-κB通路的关系(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第12期
页码:
1398-1403
栏目:
基础医学
出版日期:
2016-06-30

文章信息/Info

Title:
Resveratrol protects hepatocytes against hypoxia/reoxygenation injury and its relationship with TLR4/NF-κB pathway
作者:
何雕张国庆郑道峰魏续福刘锐吴忠均
重庆医科大学附属第一医院肝胆外科
Author(s):
He Diao Zhang Guoqing Zheng Daofeng Wei Xufu Liu Rui Wu Zhongjun

Department of Hepatobiliary Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China

关键词:
白藜芦醇缺氧/复氧肝细胞损伤Toll样受体4核因子-&kappaB
Keywords:
resveratrol hypoxia/reoxygenation hepatocyte injury toll-like receptor 4 nuclear factor-&kappaB
分类号:
R285.5; R322.47; R364.4
文献标志码:
A
摘要:

目的      探讨白藜芦醇(resveratrol,RES)在缺氧/复氧(hypoxia reoxygenation,H/R)诱导的大鼠肝细胞损伤中的保护作用及其相关分子机制。      方法      建立BRL-3A细胞(大鼠肝细胞株)H/R模型。建模前分别用RES和TLR4抑制剂(HTA125)预处理细胞。建模完成后,检测细胞存活率及细胞凋亡,观察细胞形态变化,检测细胞培养液中谷丙转氨酶(alanine transaminase, ALT)及炎症因子含量,检测细胞TLR4、NF-κB p65基因mRNA及蛋白水平表达及NF-κB p65入核情况。      结果      H/R条件使细胞存活率明显降低,细胞凋亡率增加(P<0.01),受损细胞明显增多,ALT、IL-1β含量增多(P<0.01),TLR4和NF-κB p65表达水平显著提高(P<0.01),p65入核细胞比例提高(P<0.01)。经RES及HTA125预处理后,细胞存活率显著提高,细胞凋亡比例显著缩小(P<0.01),受损细胞减少,ALT、IL-1β含量降低(P<0.01),TLR4和NF-κB p65表达水平明显降低(P<0.01),p65入核细胞比例下降(P<0.01)。      结论      RES可以减轻H/R诱导的BRL-3A肝细胞损伤,该作用可能与抑制TLR4/NF-κB信号通路有关。

Abstract:

Objective      To determine the protective effect of resveratrol (RES) on hepatocytes induced by hypoxia/reoxygenation (H/R) and investigate the molecular mechanism.       Methods      Before H/R model was established, BRL-3A cells were treated with RES or HTA125 (TLR4 inhibitor). Survival rate and apoptosis were detected in the cells. Alanine transaminase (ALT) and interleukin-1β (IL-1β) in the culture were determined. The mRNA and protein expression levels of TLR4 and NF-κB p65 in the cells were determined by quantitative real-time PCR and Western blotting.       Results       H/R stimulation decreased cell survival rate and enhanced the apoptosis (P<0.01). The expression levels of ALT and IL-1β were enhanced significantly (P<0.01), and the expression levels of TLR4 and NF-κB p65 were markedly increased (P<0.01). After pretreatment with RES or HTA125, the cell survival rate was increased (P<0.01) while the apoptosis decreased (P<0.01). ALT and IL-1β were reduced significantly (P<0.01), and the expression levels of TLR4 and NF-κB p65 were decreased (P<0.01). Moreover, RES inhibited the translocation of NF-κB p65 after H/R stimulation in BRL-3A cells (P<0.01).       Conclusion      RES can alleviate the hepatocyte injury induced by H/R, which may be related with inhibition of TLR4/NF-κB signaling pathway.

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更新日期/Last Update: 2016-06-07