[1]朱璨,节云峰,张倩,等.多孔钽颗粒负载碱性成纤维细胞生长因子促进犬颌骨缺损修复的初步研究[J].第三军医大学学报,2016,38(11):1240-1244.
 Zhu Can,Jie Yunfeng,Zhang Qian,et al.Primary study on repair of canine jaw defects with porous tantalum particles loaded with basic fibroblast growth factor[J].J Third Mil Med Univ,2016,38(11):1240-1244.
点击复制

多孔钽颗粒负载碱性成纤维细胞生长因子促进犬颌骨缺损修复的初步研究(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第11期
页码:
1240-1244
栏目:
基础医学
出版日期:
2016-06-15

文章信息/Info

Title:
Primary study on repair of canine jaw defects with porous tantalum particles loaded with basic fibroblast growth factor
作者:
朱璨节云峰张倩崔嘉玺钟建鑫汪昆王晓亮周继祥
第三军医大学西南医院口腔科;重庆润泽医药有限公司;四川省食品药品检测院
Author(s):
Zhu Can Jie Yunfeng Zhang Qian Cui Jiaxi Zhong Jianxin Wang Kun Wang Xiaoliang Zhou Jixiang

Department of Stomatology, Southwest Hospital, Third Military Medical University, Chongqing, 400038; Chongqing Runze Pharmaceutical Co., Ltd., Chongqing, 401120; Sichuan Institute for Food and Drug Control, Chengdu, Sichuan Province, 611731, China

关键词:
碱性成纤维细胞生长因子多孔钽颌骨缺损骨生成
Keywords:
basic fibroblast growth factor porous tantalum jaw bone defect osteogensis
分类号:
R318.08; R341; R782.4
文献标志码:
A
摘要:

目的      探讨碱性成纤维细胞生长因子(basic fibroblast growth factor, bFGF)对多孔钽颗粒修复犬颌骨缺损过程中的促进效果。       方法      Beagle犬6只,建立下颌骨缺损模型共计36个,单侧3个骨缺损分别为多孔钽颗粒修复组(实验组A)、bFGF+多孔钽颗粒修复组(实验组B)、无干预组(对照组)。术后4、8、12周各处死2只犬,行大体、X线、组织学观察及新生骨面积统计分析。       结果      X线片示实验组与周围骨质结合良好,对照组12周后仍可见低密度骨缺损区域。甲苯胺蓝染色示术后8周及12周实验组B骨连续性及成熟度优于实验组A。术后4周实验组B新生骨形成面积明显大于实验组A(P<0.05),而8周末实验组A明显大于实验组B(P<0.05)。       结论      bFGF可增强多孔钽颗粒的骨修复能力。

Abstract:

Objective      To evaluate the effect of basic fibroblast growth factor (bFGF) on the repair of canine jaw defects by porous tantalum particles.       Methods      A total of 36 mandibular defect models were established in 6 Beagle dogs, and the unilateral bone defects were implanted with porous tantalum (group A) and porous tantalum loaded with bFGF (group B). The control group did not perform intervention. Mandibular specimens were harvested in 4, 8 and 12 weeks postoperatively to carry out gross observation, X-ray analysis, fluorescence microscopy and histological examinations, and the ossification in the defects at different time was observed.       Results      After 12 weeks, the porous tantalum particles were surrounded by new bone in the experimental groups, and the defect area was still visible in the control group. The continuity of the bone around the porous tantalum particles in group B was superior than that in group A at the end of 12 weeks. New bone formation area in group B was greater than that in group A in 4 weeks after operation (P<0.05), while group A was greater than group B at the end of 8 weeks (P<0.05).       Conclusion      When repairing the bone defects with porous tantalum particles, bFGF can promote the osteogensis, and shorten the bone healing time.

参考文献/References:

[1]Frigg A, Dougall H, Boyd S, et al. Can porous tantalum be used to achieve ankle and subtalar arthrodesis?: a pilot study[J]. Clin Orthop Relat Res, 2010, 468(1): 209-216. DOI: 10.1007/s11999-009-0948-x
[2]Zhang Y, Li L, Shi Z J, et al. Porous tantalum rod implant is an effective and safe choice for early-stage femoral head necrosis: a meta-analysis of clinical trials[J]. Eur J Orthop Surg Traumatol, 2013, 23(2): 211-217. DOI: 10.1007/s00590-012-0962-7
[3]钟建鑫, 节云峰, 罗金英, 等. 多孔钽颗粒在下颌骨缺损修复中的作用[J]. 第三军医大学学报, 2015, 37(12): 1277-1280.DOI: 10.16016/j.1000-5404.201502120
[4]柯希煌, 练克俭, 翟文亮. 骨生长因子对骨形成的协同作用研究进展[J]. 中国矫形外科杂志, 2005, 13(20): 1582-1583.
[5]Omata K, Matsuno T, Asano K, et al. Enhanced bone regeneration by gelatin-beta-tricalcium phosphate composites enabling controlled release of bFGF[J]. J Tissue Eng Regen Med, 2014, 8(8): 604-611. DOI: 10.1002/term.1553
[6]Honnami M, Choi S, Liu I L, et al. Bone regeneration by the combined use of tetrapod-shaped calcium phosphate granules with basic fibroblast growth factor-binding ion complex gel in canine segmental radial defects[J]. J Vet Med Sci, 2014, 76(7): 955-961.DOI: 10.1292/jvms.14-0027
[7]Nagayasu T, Imamura K, Nakanishi K. Adsorption characteristics of various organic substances on the surfaces of tantalum, titanium, and zirconium[J]. J Colloid Interface Sci, 2005, 286(2): 462-470. DOI: 10.1016/j.jcis.2005.01.023
[8]Tang Z, Xie Y, Yang F, et al. Porous tantalum coatings prepared by vacuum plasma spraying enhance bmscs osteogenic differentiation and bone regeneration in vitro and in vivo[J]. PLoS One, 2013, 8(6): e66263. DOI: 10.1371/journal.pone.0066263
[9]Hayakawa K, Date H, Tsujimura S, et al. Mid-term results of total knee arthroplasty with a porous tantalum monoblock tibial component[J]. Knee, 2014, 21(1): 199-203. DOI: 10.1016/j.knee.2013.06.004
[10]Lachiewicz P F, Bolognesi M P, Henderson R A, et al. Can tantalum cones provide fixation in complex revision knee arthroplasty?[J]. Clin Orthop Relat Res, 2012, 470(1): 199-204. DOI: 10.1007/s11999-011-1888-9
[11]Dabrowski B, Swieszkowski W, Godlinski D, et al. Highly porous titanium scaffolds for orthopaedic applications[J]. J Biomed Mater Res B Appl Biomater, 2010, 95(1): 53-61. DOI: 10.1002/jbm.b.31682
[12]Su J, Xu H, Sun J, et al. Dual delivery of BMP-2 and bFGF from a new nano-composite scaffold, loaded with vascular stents for large-size mandibular defect regeneration[J]. Int J Mol Sci, 2013, 14(6): 12714-12728. DOI: 10.3390/ijms140612714
[13]Kotev-Emeth S, Pitaru S, Pri-Chen S, et al. Establishment of a rat long-term culture expressing the osteogenic phenotype: dependence on dexamethasone and FGF-2[J]. Connect Tissue Res, 2002, 43(4): 606-612.
[14]Qu D, Li J, Li Y, et al. Angiogenesis and osteogenesis enhanced by bFGF ex vivo gene therapy for bone tissue engineering in reconstruction of calvarial defects[J]. J Biomed Mater Res A, 2011, 96(3): 543-551. DOI: 10.1002/jbm.a.33009
[15]唐列云, 何爱咏. 局部注射碱性成纤维细胞生长因子对骨缺损修复的影响[J]. 广西医学, 2010, 32(1): 28-31.
[16]Shimizu A, Tajima S, Tobita M, et al. Effect of control-released basic fibroblast growth factor incorporated in beta-tricalcium phosphate for murine cranial model[J]. Plast Reconstr Surg Glob Open, 2014, 2(3): e126.DOI: 10.1097/GOX.0000000000000063
[17]He X Y, Chen Z Z, Cai Y Q, et al. Expression of cytokines in rat brain with focal cerebral ischemia after grafting with bone marrow stromal cells and endothelial progenitor cells[J]. Cytotherapy, 2011, 13(1): 46-53. DOI: 10.3109/14653249.2010.510505

相似文献/References:

[1]张倩,节云峰,朱璨,等.生物活性多孔钽骨修复材料促骨生长的实验研究[J].第三军医大学学报,2016,38(11):1245.
 Zhang Qian,Jie Yunfeng,Zhu Can,et al.Effect of TGF-β1 combined with porous tantalum on repairing maxillofacial bone defects[J].J Third Mil Med Univ,2016,38(11):1245.
[2]耿丽鑫,甘洪全,王茜,等.国产多孔钽对成骨细胞生物相容性及其相关成骨基因表达的影响[J].第三军医大学学报,2014,36(11):1163.
 Geng Lixin,Gan Hongquan,Wang Qian,et al.Effect of domestic porous tantalum on biocompatibility and osteogenic gene expression in rat osteoblasts[J].J Third Mil Med Univ,2014,36(11):1163.

更新日期/Last Update: 2016-05-29