[1]张倩,节云峰,朱璨,等.生物活性多孔钽骨修复材料促骨生长的实验研究[J].第三军医大学学报,2016,38(11):1245-1250.
 Zhang Qian,Jie Yunfeng,Zhu Can,et al.Effect of TGF-β1 combined with porous tantalum on repairing maxillofacial bone defects[J].J Third Mil Med Univ,2016,38(11):1245-1250.
点击复制

生物活性多孔钽骨修复材料促骨生长的实验研究(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第11期
页码:
1245-1250
栏目:
基础医学
出版日期:
2016-06-15

文章信息/Info

Title:
Effect of TGF-β1 combined with porous tantalum on repairing maxillofacial bone defects
作者:
张倩节云峰朱璨钟建鑫崔嘉玺汪昆王晓亮舒绍兵周继祥
第三军医大学西南医院口腔科;重庆润泽医药有限公司;四川省食品药品检测院;武警重庆总队机关门诊部口腔科
Author(s):
Zhang Qian Jie Yunfeng Zhu Can Zhong Jianxin Cui Jiaxi Wang Kun Wang Xiaoliang Shu Shaobing Zhou Jixiang

Department of Stomatology, Southwest Hospital, Third Military Medical University, Chongqing, 400038; Chongqing Runze Pharmaceutical Co., Ltd. Chongqing, 401120; Sichuan Institute for Food and Drug Control, Chengdu, Sichuan Province, 611731; Department of Stomatology, Authority Clinic of Chongqing Armed Police Force, Chongqing, 401147, China

关键词:
TGF-&beta1多孔钽骨缺损
Keywords:
TGF-&beta1 porous tantalum bone defects
分类号:
R318.08; R332; R683.05
文献标志码:
A
摘要:

目的      通过将转化生长因子-β1(transforming growth factor-β1,TGF-β1)与多孔钽颗粒复合,制备生物活性多孔钽修复材料,并观察该材料的促骨生长作用。      方法      6只Beagle犬,建立下颌骨缺损模型共计36个,单侧3个骨缺损分别为单纯多孔钽颗粒修复组(对照组)、TGF-β1+多孔钽颗粒修复组(实验组)和无干预组(空白对照组)。于材料植入后30、60、90 d处死动物,取下颌骨行大体观察、X线检测、甲苯胺蓝染色、四环素荧光标记检测、CD31相关抗原抗体染色。      结果      30 d时实验组新骨生成面积及新生微血管数目显著大于对照组和空白对照组(P<0.05);60 d时对照组新骨生成面积及新生微血管数目显著大于实验组和空白对照组(P<0.05);90 d时3个时间点成骨情况差异无统计学意义(P>0.05)。      结论      TGF-β1复合多孔钽生物材料具有高促骨活性,能加速多孔钽与周围骨组织的早期生物性结合,缩短愈合时间。

Abstract:

Objective      To prepare biological composite combining porous tantalum and TGF-β1, and observe its effect on bone growth.       Methods      A total of 36 mandibular defect models were established in 6 Beagle dogs, TGF-β1 treated porous tantalum particles and non-treated porous tantalum particles were implanted into the defect areas of bilateral mandibular bone in Beagle dogs. Blank control group was also established. Animals were sacrificed on day 30, day 60 and day 90 after implantation. Mandibular bones were examined by X-ray detection, dissection, toluidine blue staining, tetracycline fluorescence assay, and CD31 associated antigen antibody assay.       Results      Larger area of new bone and more newly formed microvessels were found in the experimental group than in the control group on day 30 and day 60 (P<0.05). On day 90, no difference was observed among the three groups (P>0.05).       Conclusion      Porous tantalum combined with TGF-β1 can effectively promote bone formation and facilitate the biological adhesion of porous tantalum to surrounding bone tissues, thus shortening the healing time.

参考文献/References:

[1]路建, 郑小平, 王志强. 多孔金属钽假体在骨缺损修复中的应用[J]. 中国修复重建外科杂志, 2012, 26(2): 244-247.
[2]钟建鑫, 节云峰, 罗金英, 等. 多孔钽颗粒在下颌骨缺损修复中的作用[J]. 第三军医大学学报, 2015, 37(12): 1277-1280. DOI: 10.16016/j.1000-5404.201502120
[3]Zhao D, Liu B, Wang B, et al. Autologous bone marrow mesenchymal stem cells associated with tantalum rod implantation and vascularized iliac grafting for the treatment of end-stage osteonecrosis of the femoral head[J]. Biomed Res Int, 2015, 2015: 240506. DOI: 10.1155/2015/240506
[4]Ferreira C L, Abreu F A, Silva G A, et al. TGF-β1 and BMP-4 carried by liposomes enhance the healing process in alveolar bone[J]. Arch Oral Biol, 2013, 58(6): 646-656. DOI: 10.1016/ j.archoralbio.2012.11.013
[5]袁晟, 张志宏. 转化生长因子-β对牙种植体骨结合的影响[J]. 医学研究生学报, 2012, 25(2): 212-214. DOI: 10.3969/j.issn. 1008-8199.2012.02.025
[6]耿丽鑫, 李琪佳. 多孔钽的生物学特性与关节重建[J]. 实用医学杂志, 2014, 30(14): 2328-2330. DOI: 10.3969/j.issn.1006-5725. 2014.14.056
[7]Moen T C, Ghate R, Salaz N, et al. A monoblock porous tantalum acetabular cup has no osteolysis on CT at 10 years[J]. Clin Orthop Relat Res, 2011, 469(2): 382-386. DOI: 10.1007/s11999- 010-1500-8
[8]Hanzlik J A, Day J S, Acknowledged Contributors: Ingrowth Retrieval Study Group. Bone ingrowth in well-fixed retrieved porous tantalum implants[J]. J Arthroplasty, 2013, 28(6): 922-927. DOI: 10.1016/j.arth.2013.01.035
[9]Khan R, Agrotis A, Bobik A. Understanding the role of transforming growth factor-beta1 in intimal thickening after vascular injury[J]. Cardiovasc Res, 2007, 74(2): 223-234. DOI: 10.1016/j.cardiores. 2007.02.012
[10]李守宏, 庞光明, 陈建钢. TGF-β1复合物诱导颌骨成骨过程中微血管的形成与表达[J]. 临床口腔医学杂志, 2007, 23(3): 137-139. DOI: 10.3969/j.issn.1003-1634.2007.03.003
[11]Costa C, Soares R, Schmitt F. Angiogenesis: now and then[J]. APMIS, 2004, 112(7/8): 402-412. DOI: 10.1111/j.1600-0463. 2004.apm11207-0802.x

相似文献/References:

[1]罗圣霖,覃容欣,周向东.血管生成抑制剂候选物——去甲苔藓蒽噻吩的设计合成[J].第三军医大学学报,2008,30(07):592.
 LUO Sheng-lin,QIN Rong-xin,ZHOU Xiang-dong.Design and synthesis of demethyl bryoanthrathiophene, a new potential angiogenesis inhibitor[J].J Third Mil Med Univ,2008,30(11):592.
[2]夏俊波,宋云熙,曾雪峰,等.1,25-二羟维生素D-3处理树突状细胞在过敏性气道炎症中的作用[J].第三军医大学学报,2008,30(08):743.
 XIA Jun-bo,SONG Yun-xi,ZENG Xue-feng,et al.Role of 1,25-Dihydroxyvitamin D3 treated dendritic cells in allergic airway inflammation[J].J Third Mil Med Univ,2008,30(11):743.
[3]胡雁,周善璧.1, 25-二羟维生素D3抑制大鼠角膜移植术后免疫排斥反应[J].第三军医大学学报,2008,30(23):2211.
 HU Yan,ZHOU Shan-bi.Prevention of corneal allograft rejection by 1, 25-dihydroxyvitamin D3 in rats[J].J Third Mil Med Univ,2008,30(11):2211.
[4]彭东红,张能.果糖二磷酸钠镁对链脲佐菌素致胰岛B细胞损伤的保护作用[J].第三军医大学学报,2006,28(24):2451.
[5]陈建明,范士志,蒋耀光,等.二氮嗪与1, 6-二磷酸果糖心麻痹液对大鼠心肌细胞的保护作用[J].第三军医大学学报,2007,29(09):786.
 CHEN Jian-ming,FAN Shi-zhi,JIANG Yao-guang,et al.Effects of cardioplegic solution supplemented with diazoxide and fructose-1,6- diphosphate on myocardial apoptosis of cryopreserved rat hearts[J].J Third Mil Med Univ,2007,29(11):786.
[6]郎海滨,糜漫天.1,25二羟维生素D3联合他莫西芬对转染VDRE-Tk-ERα表达载体的MDA-MB-231细胞周期和凋亡的影响[J].第三军医大学学报,2006,28(11):1164.
[7]陈建明,范士志,李寒,等.附加二氮嗪与1, 6-二磷酸果糖心麻痹液对冷保存大鼠心脏能量代谢的作用[J].第三军医大学学报,2006,28(09):955.
[8]杨孟雪,甘华,沈清,等.1, 25-(OH)2D3及LPS对2型糖尿病肾病尿毒症患者单核细胞维生素D受体表达的影响[J].第三军医大学学报,2011,33(16):1731.
 Yang Mengxue,Gang Hua,Sheng Qing,et al.Effect of 1,25-(OH)2D3 and LPS on expression of vitamin D receptor in human monocytes incubated with sera from type 2 diabetes patients and diabetic nephropathy patients with uremia[J].J Third Mil Med Univ,2011,33(11):1731.
[9]严媚,温丙昭,曹琰,等.1,25-(OH)2D3及复方丹参对大鼠异基因骨髓移植后T细胞亚群和细胞因子的影响[J].第三军医大学学报,2009,31(24):2436.
 YAN Mei,WEN Bing-zhao,CAO Yan,et al.Influence of 1,25-(OH)2D3 and salvia miltiorrhiza on T cell subsets and immune status in rats after allogeneic bone marrow transplantation[J].J Third Mil Med Univ,2009,31(11):2436.
[10]覃容欣,罗圣霖,周向东.苔藓蒽噻吩-1-羧酸甲酯的合成[J].第三军医大学学报,2007,29(22):2124.
 QIN Rong-xin,LUO Sheng-lin,ZHOU Xiang-dong.Highly efficient synthesis of methyl bryoanthrathiophene-1-carboxylate[J].J Third Mil Med Univ,2007,29(11):2124.

更新日期/Last Update: 2016-05-29