[1]綦英强,王三荣,晁凤蕾,等.Nω硝基左旋精氨酸甲酯对大鼠海马CA1区毛细血管的影响[J].第三军医大学学报,2016,38(12):1385-1391.
 Qi Yingqiang,Wang Sanrong,Chao Fenglei,et al.Effect of Nω-nitro-L-arginine methyl ester on capillaries of rat hippocampal CA1 region[J].J Third Mil Med Univ,2016,38(12):1385-1391.
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Nω硝基左旋精氨酸甲酯对大鼠海马CA1区毛细血管的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第12期
页码:
1385-1391
栏目:
基础医学
出版日期:
2016-06-30

文章信息/Info

Title:
Effect of Nω-nitro-L-arginine methyl ester on capillaries of rat hippocampal CA1 region
作者:
綦英强王三荣晁凤蕾张蕾马晶况超陈林木张毅梁芯唐静张杨朱殷青罗艳敏唐勇
重庆医科大学基础医学院组织学与胚胎学教研室,干细胞与组织工程学研究室
Author(s):
Qi Yingqiang Wang Sanrong Chao Fenglei Zhang Lei Ma Jing Kuang Chao Chen Linmu Zhang Yi Liang Xin Tang Jing Zhang Yang Zhu Yanqing Luo Yanmin Tang Yong

Department of Histology and Embryology, Laboratory of Stem Cells and Tissue Engineering, College of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016, China

关键词:
N&omega硝基左旋精氨酸甲酯内源性一氧化氮海马CA1区毛细血管体视学
Keywords:
L-NAME endogenous nitric oxide hippocampal CA1 region capillaries stereology
分类号:
R338.26; R338.64; R977.4
文献标志码:
A
摘要:

目的      探讨Nω硝基左旋精氨酸甲酯(Nω-nitro-左旋精氨酸 methyl ester,L-NAME)对大鼠空间学习、记忆能力和海马CA1区毛细血管的影响。      方法      内导管成功埋植后,阻断剂组(L-NAME组)大鼠每日给予5  μL的1  μmol/μL L-NAME侧脑室注射,对照组(Sham组)每日给予5  μL生理盐水侧脑室注射,持续4周。然后用Morris水迷宫测试大鼠空间学习和记忆能力,用试剂盒检测海马组织中NOS活性及NO含量,用体视学方法测定海马和海马CA1区总体积、海马CA1区毛细血管总长度、总表面积和总体积。      结果      水迷宫实验中L-NAME 组的潜伏期显著长于Sham组(P=0.006);L-NAME 组海马组织中的NOS活性及NO含量均显著低于Sham组(P<0.01);L-NAME组海马体积、CA1区体积与Sham组比较差异无统计学意义(P>0.05);L-NAME 组CA1区毛细血管总长度、毛细血管总表积、毛细血管总体积均较Sham组显著减少(P<0.05)。      结论      L-NAME可以导致大鼠空间学习、记忆能力下降和大鼠海马CA1区毛细血管总长度、总表面积以及总体积减少。

Abstract:

Objective      To determine the effect of Nω-nitro-L-arginine methyl ester (L-NAME) on the spatial learning and memory abilities and the capillaries in CA1 region of rat hippocampus.       Methods      After the catheter was successfully inserted into lateral ventricle, in the L-NAME group, 1 μmol/μL L-NAME was injected into the lateral ventricle of each rat at a dose of 5 μL once a day for 28 d, and in the sham group, the same dose of saline was injected into the lateral ventricle. Then, Morris water maze test was used to test the spatial learning and memory abilities. After the Morris water maze test, the NOS activity and NO content in the hippocampal tissues were measured. The total volume of hippocampus and hippocampus CA1 region, and the total length, total volume and total surface area of the capillaries in CA1 region were determined using stereological techniques.       Results      The water maze results showed that the time latency of the sham group was significantly shorter than that of the L-NAME group (P=0.006). Both NOS activity and NO content in the hippocampus of the L-NAME group were significantly lower than those of the sham group (P<0.01). The total volume of the hippocampus and CA1 region had no significant difference between the L-NAME group and the sham group (P>0.05). The total length, total surface area and total volume of the capillaries in the CA1 region of the L-NAME group were significantly less than those of the sham group (P<0.05).       Conclusion      L-NAME induces significant decline in the spatial learning and memory abilities of the rats and significant decrease in the total length, total surface area and total volume of the capillaries in the CA1 region of rat hippocampus.

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更新日期/Last Update: 2016-06-07