[1]刘佳佳,刘军言,严鹏,等.沉默肌切蛋白表达抑制胃癌干细胞自我更新能力[J].第三军医大学学报,2015,37(23):2324-2328.
 Liu Jiajia,Liu Junyan,Yan Peng,et al.Knockdown of scinderin attenuates self-renewal capacity in gastric cancer stem cells[J].J Third Mil Med Univ,2015,37(23):2324-2328.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第23期
页码:
2324-2328
栏目:
论著
出版日期:
2015-12-15

文章信息/Info

Title:
Knockdown of scinderin attenuates self-renewal capacity in gastric cancer stem cells
作者:
刘佳佳刘军言严鹏吴益西姬成东王艳霞向东方钱锋
第三军医大学西南医院:全军普通外科中心,微创胃肠外科中心,病理学研究所
Author(s):
Liu Jiajia Liu Junyan Yan Peng Wu Yixi Ji Chengdong Wang Yingxia Xiang Dongfang Qian Feng

Center of General Surgery, Center of Minimal Invasive Gastrointestinal Surgery, Institute of Pathology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China

关键词:
胃癌肌切蛋白干细胞自我更新
Keywords:
gastric cancer scinderin cancer stem cells self-renewal
分类号:
R341; R394.2; R735.2
文献标志码:
A
摘要:

目的      探讨肌切蛋白(scinderin,SCIN)在调控胃癌干细胞自我更新能力中的作用。      方法      采用shRNA慢病毒干扰技术构建沉默SCIN表达的MGC803胃癌细胞系和XN0422原代胃癌细胞,同时构建各自的阴性对照(mock)细胞;以平板克隆形成和成球实验分别检测沉默SCIN对胃癌细胞克隆形成和成球能力的影响;裸鼠皮下移植瘤模型观察沉默SCIN对胃癌细胞体内成瘤能力的影响。      结果      SCIN在胃癌干细胞中的表达显著高于普通贴壁细胞;沉默SCIN显著降低胃癌细胞的克隆和成球能力,MGC803细胞中 SCIN敲低细胞与mock细胞的克隆形成数分别为(26.33±4.37)和(62.33±3.18),肿瘤球形成数分别为(12.33±0.88)和(39.00±2.69),而在XN0422 细胞中,克隆形成数分别为(14.67±3.38)和(49.67±2.19),肿瘤球形成数分别为(15.67±1.76)和(33.33±1.65)(P<0.01);SCIN敲低的MGC803和 XN0422细胞较其mock细胞所形成的裸鼠皮下移植瘤,体积显著减小、质量显著减轻,差异有统计学意义(P<0.01)。      结论       SCIN基因沉默能有效抑制胃癌干细胞自我更新能力,可能成为针对胃癌肿瘤干细胞治疗的新靶点。

Abstract:

Objective      To determine the role of scinderin (SCIN) in the regulation of self-renewal capacity of gastric cancer stem cells.       Methods      SCIN-knockdown gastric cancer cells, including gastric cancer cell line MGC803 and primary gastric cancer XN0422 cells, were established with SCIN specific shRNA (shSCIN). Colony and tumorsphere formation assays were used to estimate the effect of shSCIN on self-renewal capability of gastric cancer cells in vitro, and the xenograft assay in nude mice was employed to assess the effect of shSCIN on tumorigenecity of gastric cancer cells.       Results      The expression of SCIN was markedly higher in gastric cancer stem cells than gastric cancer cells. SCIN knockdown significantly decreased the capabilities of colony and tumorsphere formation in MGC803 and XN0422 cells. The colony number derived from SCIN knockdown and mock cells was 26.33±4.37 and 62.33±3.18 in MGC803 cells, and 14.67±3.38 and 49.67±2.19 in XN0422 cells, respectively. The number of tumorsphere formatted by SCIN knockdown and mock cells was 12.33±0.88 and 39.00±2.69 in MGC803 cells, and 15.67±1.76 and 33.33±1.65 in XN0422 cells (P<0.01 for all), respectively. Xenograft assay showed that the volume and weight of the tumor mass derived from SCIN-knockdown MGC803 and XN0422 cells were remarkably smaller/lighter than that of mock cells (P<0.05 for all).       Conclusion      Silencing of SCIN can effectively inhibit the self-renewal of gastric cancer stem cells, suggesting that SCIN might be a promising target for gastric cancer therapy by targeting gastric cancer stem cells.

参考文献/References:

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更新日期/Last Update: 2015-11-30