[1]邓皓月,糜漫天.白藜芦醇通过上调SIRT1/自噬通路改善Aβ25-35对PC12细胞增殖的抑制作用[J].第三军医大学学报,2016,38(06):619-622.
 Deng Haoyue,Mi Mantian.Resveratrol attenuates Aβ25-35 induced neurotoxicity via activation of SIRT1/autophagy signaling pathway[J].J Third Mil Med Univ,2016,38(06):619-622.
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白藜芦醇通过上调SIRT1/自噬通路改善Aβ25-35对PC12细胞增殖的抑制作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第06期
页码:
619-622
栏目:
基础医学
出版日期:
2016-03-30

文章信息/Info

Title:
Resveratrol attenuates Aβ25-35 induced neurotoxicity via activation of SIRT1/autophagy signaling pathway
作者:
邓皓月糜漫天
第三军医大学军事预防医学院营养与食品卫生学教研室,营养与食品安全研究中心,重庆市营养与食品安全重点实验室,重庆市医学营养研究中心
Author(s):
Deng Haoyue Mi Mantian

Department of Nutrition and Food Hygiene Research Center for Nutrition and Food Safety, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing Center of Medical Nutrition, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China

关键词:
自噬SIRT1白藜芦醇神经细胞毒性
Keywords:
autophagy SIRT1 resveratrol neurotoxicity
分类号:
R151.3;R322.81;R741.05
文献标志码:
A
摘要:

目的      探讨SIRT1/自噬通路在白藜芦醇改善Aβ25-35诱导的PC12细胞增殖活力下降中的作用。      方法      用不同浓度(1、5、10、20 μmol/L)白藜芦醇 (resveratrol, RSV) 单独或加入5 mmol/L自噬特异抑制剂(3-methyladenine, 3-MA)、2 μmol/L SIRT1选择性抑制剂(EX527)处理PC12细胞 2 h后,再加入30 μmol/L淀粉样蛋白25-35片段(Amyloid β-protein fragment 25-35, Aβ25-35 )处理24 h。采用CCK-8检测细胞增殖活力,电镜检测自噬体,Western blot检测自噬标志蛋白P62、LC3和SIRT1蛋白的表达水平。      结果      30 μmol/L Aβ25-35可显著降低PC12细胞的增殖活力,为对照组的45.14%(P<0.05)。RSV预处理可显著改善Aβ25-35 诱导的PC12细胞增殖活力下降(P<0.05),且随浓度增加,保护作用增强。同时,RSV可明显促进自噬体的形成,上调SIRT1、LC3-Ⅱ的表达和降低P62的表达,且随浓度增加,表达变化越显著;而加入3-MA和EX527处理能显著抑制RSV的上述效应。      结论      RSV可通过激活SIRT1/自噬通路改善Aβ25-35诱导的神经细胞毒性。

Abstract:

Objective      To determine whether resveratrol (RSV) attenuates Aβ25-35 induced neurotoxicity through SIRT1/autophagy signaling pathway.       Methods      PC12 cells were treated with different concentrations (1, 5, 10 and 20 μmol/L) of RSV for 2 h in the presence or absence of 5 mmol/L 3-methyladenine (3-MA, specific inhibitor of autophagy ) or 2 μmol/L EX527 (selective inhibitor of SIRT1), following by treatment of 30 μmol/L amyloid β-protein fragment 25-35 (Aβ25-35 ) for 24 h. CCK-8 assay was used to detect the cell viability, and electron microscope was applied to detect autophagosome. The expressions of P62, LC3 and SIRT1 were detected by Western blotting.       Results      Aβ25-35 (30 μmol/L) remarkably decreased the viability of PC12 cells, which was 45.14% of the control group P<0.05). Pretreatment with RSV significantly improved Aβ25-35 induced decrease of cell viability in a dose-dependent manner (P<0.05). Simultaneously, RSV promoted the formation of autophagosome, up-regulated the expressions of SIRT1 and LC3-Ⅱ and down-regulated the expression of P62 in a dose-dependent manner. However 3-MA and EX527 abolished the above effects of RSV.       Conclusion      RSV attenuates Aβ25-35 induced neurotoxicity through activation of SIRT1/autophagy signaling pathway.

相似文献/References:

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 Zhou Xi,Yi Long,Jin Xin,et al.Role of SIRT1/UCP2 signaling pathway in resveratrol-induced inhibition of oxidative injury in vascular endothelial cells[J].J Third Mil Med Univ,2013,35(06):1671.
[2]梅娟娟,田曼,严莎莎,等.呼吸道合胞病毒通过调控miR-409-3p/SIRT1通路促进支气管上皮细胞凋亡与炎症因子表达[J].第三军医大学学报,2021,43(06):503.
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更新日期/Last Update: 2016-03-22