[1]姜锐,杨鹏,江亚萍.TNFR2基因敲除小鼠H22移植瘤生长特点和Treg数量与功能检测[J].第三军医大学学报,2016,38(01):50-54.
 Jiang Rui,Yang Peng,Jiang Yaping.Tumor growth characteristics in H22 hepatocarcinoma cells transplanted tumor and number and function of Treg in TNFR2 knockout mice[J].J Third Mil Med Univ,2016,38(01):50-54.
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TNFR2基因敲除小鼠H22移植瘤生长特点和Treg数量与功能检测(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
38卷
期数:
2016年第01期
页码:
50-54
栏目:
基础医学
出版日期:
2016-01-15

文章信息/Info

Title:
Tumor growth characteristics in H22 hepatocarcinoma cells transplanted tumor and number and function of Treg in TNFR2 knockout mice
作者:
姜锐杨鹏江亚萍
武汉市第一医院检验科;贵州省骨科医院检验科;华中科技大学同济医学院免疫学研究所
Author(s):
Jiang Rui Yang Peng Jiang Yaping

Department of Clinical Laboratory, Wuhan First Hospital, Wuhan, Hubei Province, 430022; Department of Clinical Laboratory, Guizhou Provincial Orthopaedic Hospital, Guiyang, Guizhou Province, 550007; Institute of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430030, China

关键词:
跨膜型TNF-&alpha调节性T细胞肝癌白细胞介素10转化生长因子&beta
Keywords:
transmembrane TNF-&alpha regulatory T cells tumor TNF-&alpha IL-10 TGF-&beta
分类号:
R392.2; R730.23; R735.7
文献标志码:
A
摘要:

目的      观察tmTNF-α/TNFR2信号轴对荷瘤鼠肿瘤生长影响及其机制。      方法      采用Western blot检查H22荷瘤鼠肿瘤微环境中Treg细胞TNFR1及TNFR2的表达。TNFR2敲除BALB/c小鼠和野生型鼠各10只,在TNFR2敲除的BALB/c小鼠皮下接种H22肝癌细胞株,观察肿瘤直径及肿瘤微环境中Treg的募集数量,同时以野生型小鼠做对照。用tmTNF-α刺激Treg细胞,ELISA检测上清IL-10和TGF-β的含量。      结果      荷瘤鼠微环境中Treg细胞TNFR2表达明显高于野生型小鼠脾脏Treg细胞(P<0.05),而TNFR1表达未见明显变化;TNFR2基因敲除鼠肿瘤直径明显低于野生型小鼠(P<0.05),且肿瘤微环境中Treg细胞数量也明显少于野生型小鼠(P<0.05)。在体外tmTNF-α可以促进Treg细胞产生IL-10和TGF-β(P<0.05)。      结论      TNFR2基因敲除小鼠肿瘤生长减缓,肿瘤局部微环境中Treg细胞减少,同时tmTNF-α能够刺激Treg细胞释放抑炎因子IL-10和TGF-β,增强其免疫抑制功能。

Abstract:

Objective      To determine the effect of the transmembrane TNF-α/tumor necrosis factor receptor 2  (tmTNF-α/TNFR2) signaling axis on the growth of transplantation tumor in mouse xenograft model and investigate the underlying mechanism.       Methods      Western blotting was used to determine the expression of TNFR1 and TNFR2 in the Treg cells from control mice and H22 tumor-bearing mice. TNFR2 knock-out mice and wild-type mice (10 animals in each group) were subcutaneously inoculated with H22 hepatocarcinoma cells, then the tumor growth as well as the accumulation of Treg in the suspension of tumor cells were observed. IL-10 and TGF-β production in Treg cells in response to the exogenous tmTNF-α were detected by ELISA.       Results      The TNFR2 expression was significantly higher in the Treg cells from the tumor microenvironment in the tumor-bearing mice than in the spleen Treg cells from wild-type mice (P<0.05), but no significant change was seen in the TNFR1 expression. Tumor size was obviously smaller in the TNFR2-/- tumor-bearing mice than the wild-type tumor-bearing mice (P<0.05), and the percentage of Treg in the tumor microenvironment was remarkably less in the former group of mice than the latter (P<0.05). Exogenous tmTNF-α promoted the secretion of IL-10 and TGF-β in Treg cells (P<0.05).       Conclusion       The transplanted tumor gowns slowly in TNFR2 knockout mice, with reduced Treg cells in the tumor microenvironments. tmTNF-α stimulates the release of anti-inflammatory cytokines IL-10 and TGF-β in the Treg cells to enhance the immune response.

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更新日期/Last Update: 2016-01-06