[1]于超,李艮平,陈鸿雁.硫链丝菌肽抑制FOXM1表达后对裸鼠鼻咽癌移植瘤的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2015,37(20):2038-2042.
 Yu Chao,Li Genping,Chen Hongyan.Effect of thiostrepton on inhibiting human nasopharyngeal carcinoma through down-regulating FoxM1 expression in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2015,37(20):2038-2042.
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第20期
页码:
2038-2042
栏目:
论著
出版日期:
2015-10-30

文章信息/Info

Title:
Effect of thiostrepton on inhibiting human nasopharyngeal carcinoma through down-regulating FoxM1 expression in nude mice
作者:
于超李艮平陈鸿雁
重庆市中医院耳鼻喉科;重庆医科大学附属第一医院耳鼻喉科;第三军医大学大坪医院野战外科研究所耳鼻咽喉科
Author(s):
Yu Chao Li Genping Chen Hongyan

Department of Otolaryngology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, 400021;Department of Otolaryngology, the First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016;Department of Otolaryngology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China

关键词:
鼻咽癌FoxM1硫链丝菌肽裸鼠移植瘤
Keywords:
nasopharyngeal carcinoma forkhead box M1 thiostrepton nude mice transplantation model of carcinoma
分类号:
R73-361;R739.63;R979.14
文献标志码:
A
摘要:

目的        探讨硫链丝菌肽(thiostrepton,TST)抑制叉头框M1(forkhead box M1,FoxM1)表达后对鼻咽癌CNE-2细胞裸鼠移植瘤生长的影响及可能机制。      方法      建立人鼻咽癌CNE-2细胞裸鼠移植瘤模型,将成瘤裸鼠20只放于一起,10只裸鼠作为对照组,剩余的10只作为实验组,各组裸鼠均采用耳钉法标号;腹腔给药,对照组给予DMSO溶液,实验组每周腹腔注射DMSO溶解的TST溶液(200 μg/kg);观察各组裸鼠移植瘤的生长情况,绘制生长曲线,最后瘤体称重计算抑瘤率;成瘤后取瘤体组织制成细胞悬液,流式细胞术检测细胞周期变化及细胞凋亡发生情况;提取瘤体组织蛋白进行Western blot,检测相关蛋白表达变化情况。      结果      与对照组相比,TST药物组对人鼻咽癌 CNE-2细胞裸鼠移植瘤生长具有明显的抑制作用,差别有统计学意义(P<0.05);TST药物组瘤体质量为(0.582±0.172)g,对照组瘤体质量为(1.118±0.159)g,TST组抑瘤率为47.94%,差别有统计学意义(F=31.4,P<0.01);FCM 结果表明,肿瘤组织细胞 G1期的细胞比例:对照组(47.23±5.68)%,TST药物组(66.44±3.65)%,差别有统计学意义(F=48.6,P<0.01);瘤体中细胞的凋亡率:对照组(6.12±0.95)%,TST药物组(16.99±1.75)%,两组相比较差别有统计学意义(F=179.1,P<0.01);Western blot 结果显示:TST组与对照组相比,FoxM1蛋白和Cyclin D1、Skp2蛋白表达下降,P27蛋白表达增加。      结论      硫链丝菌肽可以通过拮抗FoxM1表达,有效抑制人鼻咽癌CNE-2细胞裸鼠移植瘤的生长。

Abstract:

Objective      To investigate the inhibitory effect of thiostrepton (TST) on human nasopharyngeal carcinoma transplanted in nude mice through inhibiting forkhead box M1 (FoxM1) expression.       Methods      Human nasopharyngeal carcinoma cells CNE-2 were xenografted into nude mice, and then the tumor-bearing mice were randomly divided into control group and TST treatment group (200 μg/kg). The control group was treated with dimethyl sulfoxide (DMSO) solution, while the TST treatment group was administrated with DMSO solution containing TST by peritoneal injection. Tumor growth was observed, and the tumor was weighed to calculate tumor inhibitory rate. The apoptotic rate and cell cycle changes were analyzed by flow cytometry. Expression of FoxM1 protein and apoptosis associated proteins were detected by Western blotting.       Results      The mean tumor volume in the TST treatment group was significantly smaller than that in the control group (P<0.05). The tumor was 0.582±0.172 g in the TST treatment group and 1.118±0.159 g in the control group, and the tumor inhibitory rate of the TST treatment group was 47.94% (P<0.05). Flow cytometry indicated that the apoptotic rate [(16.99±1.75)%] and the percentage [(66.44±3.65)%] of G1-phase cells in the TST treatment group were significantly higher than those in the control group [(6.12±0.95)%, F=179.1, P<0.01; (47.23±5.68)%, F=48.6, P<0.01]. Western blotting showed that the expression of FoxM1 protein and apoptosis associated proteins (Cyclin D1 and Skp2) was decreased, but that of P27 protein was increased in the TST treatment group.       Conclusion      TST can effectively inhibit human nasopharyngeal carcinoma (CNE-2 cell line) transplanted in nude mice through down-regulating FoxM1 expression.

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 Lin Li,Deng Bi,Shou Zhu,et al.Effect of thiostrepton on proliferation and FoxM1 expression in human laryngocarcinoma Hep-2 cell line[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(20):2086.
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更新日期/Last Update: 2015-10-22