[1]韩广玮,申雪晴,高文宏,等.超声复合微泡促进骨髓间充质干细胞归巢对大鼠前列腺炎的治疗作用[J].第三军医大学学报,2015,37(11):1108-1113.
 Han Guangwei,Shen Xueqing,Gao Wenhong,et al.Efficiency of prostate-targeted homing of bone marrow mesenchymal stem cells in treatment of rat prostatitis mediated by microbubble-enhanced ultrasound[J].J Third Mil Med Univ,2015,37(11):1108-1113.
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超声复合微泡促进骨髓间充质干细胞归巢对大鼠前列腺炎的治疗作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
37卷
期数:
2015年第11期
页码:
1108-1113
栏目:
论著
出版日期:
2015-06-15

文章信息/Info

Title:
Efficiency of prostate-targeted homing of bone marrow mesenchymal stem cells in treatment of rat prostatitis mediated by microbubble-enhanced ultrasound
作者:
韩广玮申雪晴高文宏崔栋尚永刚刘城城易善红
第三军医大学新桥医院:泌尿外科,超声科;解放军169医院泌尿外科
Author(s):
Han Guangwei Shen Xueqing Gao Wenhong Cui Dong Shang Yonggang Liu Chengcheng Yi Shanhong

Department of Urology, Department of Ultrasonography, Xinqiao Hospital,Third Military Medical University, Chongqing, 400037; Department of Urology, No.169 Hospital of PLA, Hengyang, Hunan Province, 421002, China

关键词:
骨髓间充质干细胞细菌性前列腺炎炎症因子TNF-&alphaIL-1&beta大鼠超声微泡
Keywords:
bone marrow mesenchymal stem cells bacterial prostatitis inflammatory factor TNF-&alpha IL-1&beta rats ultrasound microbubble
分类号:
R329.2; R445.1; R697.33
文献标志码:
A
摘要:

目的      研究静脉注射骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)对大鼠前列腺炎的治疗作用。      方法      贴壁法分离、培养、扩增BMSCs。将50只SD大鼠按随机数字表法分为慢性细菌性前列腺炎(chronic bacterial prostatitis,CBP)组、CBP+BMSCs组、CBP+超声复合微泡(microbubble-enhanced therapeutic ultrasound,MEUS)组、CBP+MEUS+BMSCs组和正常对照组,每组10只。在小动物超声引导下向大鼠前列腺两侧叶内注射大肠埃希菌建立前列腺炎症模型,注入细菌1~14 d为急性炎症期,4~12周为慢性炎症期,对照组注入等量PBS。建模28 d后经静脉单纯注入BMSCs、单纯行MEUS及行MEUS+注入BMSCs,活体成像观察BMSCs向前列腺归巢情况。观察移植BMSCs 2周后大鼠前列腺组织病理学变化并作炎症评分,RT-PCR检测炎症因子IL-1β、TNF-α mRNA,ELISA检测IL-1β、TNF-α蛋白含量,统计分析各组表达差异。      结果      前列腺组织病理学提示CBP组、CBP+BMSCs组、CBP+MEUS组前列腺呈典型炎症病理变化,腺管上皮增生,层次增加,炎细胞浸润,纤维组织增生,而CBP+MEUS+BMSCs组大鼠前列腺炎症明显减轻。RT-PCR检测结果提示CBP组炎症因子IL-1β(0.282±0.067)、TNF-α(0.631±0.116),CBP+BMSCs组IL-1β(0.893±0.117)、TNF-α(0.876±0.137),CBP+MEUS组IL-1β(0.683±0.097)、TNF-α(0.814±0.127)高于正常对照组IL-1β(0.282±0.067)、TNF-α(0.256±0.059)和CBP+MEUS+BMSCs组IL-1β(0.322±0.086)、TNF-α(0.313±0.079)(P<0.05);ELISA检测结果显示CBP组炎症因子IL-1β[(315.14±86.08)pg/mL]、TNF-α[(86.41±24.32)pg/mL],CBP+BMSCs组IL-1β[(278.87±72.04)pg/mL]、TNF-α[(67.77±16.98)pg/mL],CBP+MEUS组IL-1β[(321.24±93.12)pg/mL]、TNF-α[(79.14±19.17)pg/mL]含量均显著高于正常对照组[IL-1β(43.36±11.35)pg/mL、TNF-α(13.26±3.97)pg/mL]和CBP+MEUS+BMSCs组[IL-1β(65.34±17.23)pg/mL、TNF-α(19.94±5.37)pg/mL],差异有统计学意义(P<0.01),而CBP+MEUS+BMSCs组与正常对照组比较差异无统计学意义(P>0.05)。      结论      超声复合微泡促进BMSCs向前列腺归巢,能够减轻前列腺炎症反应,可能为前列腺炎的治疗带来新策略。

Abstract:

Objective       To investigate the efficiency of venous injection of bone marrow mesenchymal stem cells (BMSCs) in treatment of rat prostatitis.       Methods      Rat BMSCs were separated with successive adherence method, and then cultured and amplified. Fifty SD rats were randomized into 5 groups (n=10): chronic bacterial prostatitis (CBP) group, CBP+BMSCs group, CBP+microbubble-enhanced therapeutic ultrasound (MEUS) group, CBP+MEUS+BMSCs group and normal control group. The rats in the other 4 groups except for control were injected with E.coli into both sides of prostate under ultrasound guidance to establish prostatitis model (1-14 d after injection was regarded as acute inflammation period, and 4-12 weeks as chronic inflammation period). The rats from control group were injected with the same volume of PBS. Intravenous injection of BMSCs, MEUS and MEUS+BMSCs was performed into prostatitis rats in 28 d after E.coli injection. BMSCs homing to the prostate were observed by small living animal imaging technology. Pathomorphological changes of rat prostate tissue were observed by light microscopy in 2 weeks after BMSCs injection. The mRNA and protein levels of IL-1β and TNF-α in the prostates were determined by RT-PCR and ELISA, respectively.       Results      Histopathological evaluation showed that typical pathological inflammation were observed in CBP, CBP+BMSCs and CBP+MEUS groups, including the change of gland structure, interstitial edema, inflammatory cells infiltration and fibrous hyperplasia, while pathological changes were relieved obviously in CBP+MEUS+BMSCs group. The mRNA expression levels of IL-1β and TNF-α were obviously higher in CBP group (0.282±0.067 and 0.631±0.116), CBP+BMSCs group (0.893±0.117 and 0.876±0.137), and CBP+MEUS group (0.683±0.097 and 0.814±0.127) than those in control group (0.282±0.067 and 0.256±0.059) and CBP+MEUS+BMSCs group (0.322±0.086 and 0.313±0.079, all P<0.05). The protein contents of IL-1β and TNF-α were remarkably higher in CBP group (315.14±86.08 and 86.41±24.32 pg/mL), CBP+BMSCs group (278.87±72.04 and 67.77±16.98 pg/mL), and CBP+MEUS group (321.24±93.12 and 79.14±19.17 pg/mL) than those in control group (43.36±11.35 and 13.26±3.97 pg/mL) and CBP+MEUS+BMSCs group (65.34±17.23 and 19.94±5.37 pg/mL, all P<0.01). While, there was no statistically difference in control group and CBP+MEUS+BMSCs group (P>0.05).       Conclusion      MEUS promotes the prostate-targeted transplantation of BMSCs, which can reduce the prostatitis-induced inflammation reaction. This may bring a new strategy for the treatment of prostatitis.

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更新日期/Last Update: 2015-05-27